A5054029950- Advanced Proteomics Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Extracellular vesicles in disease
- Blood Pressure and Hypertension Studies
- Chronic Kidney Disease and Diabetes
- Mass Spectrometry Techniques and Applications
- Cardiac Valve Diseases and Treatments
- Atherosclerosis and Cardiovascular Diseases
- Protease and Inhibitor Mechanisms
- Lipoproteins and Cardiovascular Health
- Dialysis and Renal Disease Management
- Aortic Disease and Treatment Approaches
- Infective Endocarditis Diagnosis and Management
- Electrochemical Analysis and Applications
- Adipokines, Inflammation, and Metabolic Diseases
- Aortic aneurysm repair treatments
- Hormonal Regulation and Hypertension
- Renin-Angiotensin System Studies
- Renal Diseases and Glomerulopathies
- Acute Kidney Injury Research
- Erythrocyte Function and Pathophysiology
- Cancer, Lipids, and Metabolism
- Metabolism, Diabetes, and Cancer
- Blood properties and coagulation
- Blood Coagulation and Thrombosis Mechanisms
Hospital Universitario Fundación Jiménez Díaz
2015-2024
Universidad Autónoma de Madrid
2015-2024
Universidad Complutense de Madrid
2010-2024
Instituto de Salud Carlos III
2016-2022
Marqués de Valdecilla University Hospital
2022
Spanish Clinical Research Network
2017-2021
Servicio de Salud de Castilla La Mancha
2010-2018
Hospital Nacional de Parapléjicos
2017-2018
Communications & Power Industries (United States)
2017
Universidad Europea
2017
Adipose tissue is an endocrine organ involved in storage and release of energy but also regulation metabolism other organs via secretion peptide protein hormones (adipokines). Especially visceral adipose has been implicated the development metabolic syndrome type 2 diabetes. Factors secreted by stromal-vascular fraction contribute to secretome modulate adipokine adipocytes. Therefore, we aimed at characterization rather than adipocyte cell secretome. The presence serum proteins intracellular...
BACKGROUND: The metabolic alterations occurring within the arterial architecture during atherosclerosis development remain poorly understood, let alone those particular to each tunica. We aimed first identify, in a spatially resolved manner, specific changes plaque, media, adventitia, and cardiac tissue between control atherosclerotic murine aortas. Second, we assessed their translatability human plasma for cardiovascular risk estimation. METHODS: In this observational study, mass...
Saliva is an easy accessible plasma ultra-filtrate. Therefore, saliva can be attractive alternative to blood for measurement of diagnostic protein markers. Our aim was determine stability and composition saliva. Protein at room temperature examined by incubating fresh whole with without inhibitors proteases bacterial metabolism followed Surface Enhanced Laser Desorption/Ionization (SELDI) analyses. determined sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) fractionation...
Coronary atherosclerosis still represents the major cause of mortality in western societies. Initiation occurs within intima, where histological and molecular changes are produced during pathogenesis. So far, proteomic analysis atherome plaque has been mainly tackled by entire tissue, which may be a challenging approach because great complexity this sample terms layers cell type composition. Based on this, we aimed to study intimal proteome from human atherosclerotic coronary artery. For...
Urinary exosomes have been proposed as potential diagnostic tools. TNF superfamily cytokines and receptors may be present in are expressed by proximal tubular cells. We now studied the expression of selected proteins exosome-like vesicles from cultured human cells urine identified additional these LC-MS/MS proteomics. Human constitutively released that did not contain TRAIL or TWEAK. However, contained osteoprotegerin (OPG), a receptor protein, assessed Western blot, ELISA reaction...
Resistant albuminuria, developed under adequate chronic blockade of the renin-angiotensin system, is a clinical problem present in small number patients with kidney disease (CKD). The mechanism underlying this resistant albuminuria remains unknown. Matrix metalloproteinases (MMPs) are involved pathophysiology cardiovascular and renal diseases. In study we tested role MMPs albuminuria. First evaluated gelatinase MMP-2 MMP-9 activity by zymography Munich Wistar Frömter (MWF) rat, model...
Discovery of new biomarkers is critical for early diagnosis acute coronary syndrome (ACS). Recent advances in metabolomic technologies have drastically enhanced the possibility improving knowledge its physiopathology through identification altered metabolic pathways. In this study, analyses peripheral plasma from non-ST segment elevation ACS patients and healthy controls by gas chromatography-mass spectrometry (GC-MC) permitted 15 metabolites with statistical differences (p < 0.05) between...
Resistant hypertension (RH) affects 9% to 12% of hypertensive adults. Prolonged exposure suboptimal blood pressure control results in end-organ damage and cardiovascular risk. Spironolactone is the most effective drug for treatment, but not all patients respond side effects are negligible. Little known on mechanisms responsible RH. We aimed identify metabolic alterations urine. In addition, a potential capacity metabolites predict response spironolactone was investigated. Urine collected...
We pursued here the identification of specific signatures proteins and metabolites in urine which respond to atherosclerosis development, acute event and/or recovery. An animal model (rabbit) was developed molecules responding silent development were identified. Those investigated human from patients suffering an coronary syndrome (ACS), at onset discharge. Kallikrein1 (KLK1) zymogen granule protein16B (ZG16B) proteins, l-alanine, l-arabitol, scyllo-inositol, 2-hydroxyphenilacetic acid,...
Thoracic aortic aneurysm (TAA) is mainly sporadic and with higher incidence in the presence of a bicuspid valve (BAV) for unknown reasons. The lack drug therapy to delay TAA progression lies limited knowledge pathophysiology. We aimed identify molecular hallmarks that differentiate dilatation associated BAV tricuspid (TAV). Aortic vascular smooth muscle cells (VSMCs) isolated from patients or TAV were analyzed by mass spectrometry. DNA oxidative damage assay cell cycle profiling performed...
Acute coronary syndrome (ACS) is triggered by the occlusion of a artery usually due to thrombosis caused an atherosclerotic plaque. The identification proteins directly involved in pathophysiological events underlying ACS will enable more precise diagnoses and accurate prognosis be determined. Accordingly, we have performed longitudinal study plasma proteome patients 2-DE DIGE. Plasma samples from patients, healthy controls, stable disease (CAD) were immunodepleted six most abundant...