A5052366300- Advanced Proteomics Techniques and Applications
- Cardiac Valve Diseases and Treatments
- Metabolomics and Mass Spectrometry Studies
- Blood Pressure and Hypertension Studies
- Protease and Inhibitor Mechanisms
- Atherosclerosis and Cardiovascular Diseases
- Lipoproteins and Cardiovascular Health
- Infective Endocarditis Diagnosis and Management
- Chronic Kidney Disease and Diabetes
- Adipokines, Inflammation, and Metabolic Diseases
- Mass Spectrometry Techniques and Applications
- Extracellular vesicles in disease
- Psoriasis: Treatment and Pathogenesis
- Cardiovascular Function and Risk Factors
- Cardiomyopathy and Myosin Studies
- Cardiovascular Disease and Adiposity
- Blood properties and coagulation
- Dialysis and Renal Disease Management
- Renin-Angiotensin System Studies
- Advanced Biosensing Techniques and Applications
- Hormonal Regulation and Hypertension
- Cancer, Lipids, and Metabolism
- S100 Proteins and Annexins
- Erythrocyte Function and Pathophysiology
- Cell Adhesion Molecules Research
Servicio de Salud de Castilla La Mancha
2016-2025
Hospital Nacional de Parapléjicos
2015-2024
Uppsala University
2022
Medical University of South Carolina
2022
Hacettepe University
2022
Centre international de recherche sur le cancer
2022
Rega Institute for Medical Research
2022
KU Leuven
2022
Complejo Hospitalario Universitario de Toledo
2022
Universidad Complutense de Madrid
2010-2017
Background The Kennedy pathway generates phosphocoline and phosphoethanolamine through its two branches. Choline Kinase (ChoK) is the first enzyme of branch synthesis phosphocholine, major component plasma membrane. ChoK family proteins composed by ChoKα ChoKβ isoforms, one with different variants splicing. Recently has been implicated in carcinogenic process, since it over-expressed a variety human cancers. However, no evidence for role carcinogenesis reported. Methodology/Principal...
Coronary atherosclerosis still represents the major cause of mortality in western societies. Initiation occurs within intima, where histological and molecular changes are produced during pathogenesis. So far, proteomic analysis atherome plaque has been mainly tackled by entire tissue, which may be a challenging approach because great complexity this sample terms layers cell type composition. Based on this, we aimed to study intimal proteome from human atherosclerotic coronary artery. For...
Degenerative aortic stenosis is the most common worldwide cause of valve replacement. While it shares certain risk factors with coronary artery disease, not delayed or reversed by reducing exposure to (e.g., therapies that lower lipids). Therefore, necessary better understand its pathophysiology for preventive measures be taken. In this work, samples were collected from 20 patients underwent replacement (55% males, mean age 74 years) and normal control valves obtained necropsies (40% 69...
Resistant albuminuria, developed under adequate chronic blockade of the renin-angiotensin system, is a clinical problem present in small number patients with kidney disease (CKD). The mechanism underlying this resistant albuminuria remains unknown. Matrix metalloproteinases (MMPs) are involved pathophysiology cardiovascular and renal diseases. In study we tested role MMPs albuminuria. First evaluated gelatinase MMP-2 MMP-9 activity by zymography Munich Wistar Frömter (MWF) rat, model...
Discovery of new biomarkers is critical for early diagnosis acute coronary syndrome (ACS). Recent advances in metabolomic technologies have drastically enhanced the possibility improving knowledge its physiopathology through identification altered metabolic pathways. In this study, analyses peripheral plasma from non-ST segment elevation ACS patients and healthy controls by gas chromatography-mass spectrometry (GC-MC) permitted 15 metabolites with statistical differences (p < 0.05) between...
Summary Membrane microvesicles (MVs) are released from activated cells, most notably platelets, into the circulation. They represent an important mode of intercellular communication, and their number is increased in patients with acute coronary syndromes. We present here a differential proteomic analysis plasma MVs ST-elevation myocardial infarction (STEMI) stable artery disease (SCAD) controls. The objective was identification biomarkers/drug targets that could be relevant for pathogenesis...
Resistant hypertension (RH) affects 9% to 12% of hypertensive adults. Prolonged exposure suboptimal blood pressure control results in end-organ damage and cardiovascular risk. Spironolactone is the most effective drug for treatment, but not all patients respond side effects are negligible. Little known on mechanisms responsible RH. We aimed identify metabolic alterations urine. In addition, a potential capacity metabolites predict response spironolactone was investigated. Urine collected...
We pursued here the identification of specific signatures proteins and metabolites in urine which respond to atherosclerosis development, acute event and/or recovery. An animal model (rabbit) was developed molecules responding silent development were identified. Those investigated human from patients suffering an coronary syndrome (ACS), at onset discharge. Kallikrein1 (KLK1) zymogen granule protein16B (ZG16B) proteins, l-alanine, l-arabitol, scyllo-inositol, 2-hydroxyphenilacetic acid,...
Abstract Aortic stenosis (AS) is the most common form of valve disease. Once symptoms develop, there an inexorable deterioration with a poor prognosis; currently are no therapies capable modifying disease progression and aortic replacement only available treatment. Our goal to study calcification by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) get new insights at molecular level that could help in understanding this In work, we analyzed consecutive slices...
Acute coronary syndrome (ACS) is triggered by the occlusion of a artery usually due to thrombosis caused an atherosclerotic plaque. The identification proteins directly involved in pathophysiological events underlying ACS will enable more precise diagnoses and accurate prognosis be determined. Accordingly, we have performed longitudinal study plasma proteome patients 2-DE DIGE. Plasma samples from patients, healthy controls, stable disease (CAD) were immunodepleted six most abundant...
Abstract Degenerative aortic stenosis (AS) is the most common worldwide cause of valve replacement. The a thin, complex, layered connective tissue with compartmentalized extracellular matrix (ECM) produced by specialized cell types, which directs blood flow in one direction through heart. There evidence suggesting remodeling such ECM during development. Thus, better characterization role proteins this disease would increase our understanding underlying molecular mechanisms. Aortic samples...
Albuminuria is an indicator of cardiovascular risk and renal damage in hypertensive individuals. Chronic renin-angiotensin system (RAS) suppression facilitates blood pressure control prevents development new-onset-albuminuria. A significant number patients, however, develop albuminuria despite chronic RAS blockade, the physiopathological mechanisms are underexplored. Urinary exosomes reflect pathological changes taking place kidney. The objective this work was to examine exosomal protein...