A5076938657- Hemoglobinopathies and Related Disorders
- Iron Metabolism and Disorders
- Viral gastroenteritis research and epidemiology
- Blood groups and transfusion
- Animal Virus Infections Studies
- Blood donation and transfusion practices
- HIV/AIDS Research and Interventions
- Trace Elements in Health
- Enzyme Structure and Function
- Hepatitis B Virus Studies
- Biochemical and biochemical processes
- Liver Disease Diagnosis and Treatment
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Hepatitis C virus research
- Glycosylation and Glycoproteins Research
- Bacteriophages and microbial interactions
- GABA and Rice Research
- Erythropoietin and Anemia Treatment
- Biochemical and Molecular Research
- Viral Infections and Immunology Research
- Academic Research in Diverse Fields
- Cytokine Signaling Pathways and Interactions
- Science and Education Research
- Blood disorders and treatments
- Animal Nutrition and Physiology
University of Cambridge
2012-2022
Centro de Investigación en Sanidad Animal
2004-2020
Centro Regional de Selección y Reproducción Animal
2007-2015
Universidade de São Paulo
2003-2014
Universidade de Ribeirão Preto
2011-2014
Clinics Hospital of Ribeirão Preto
2003-2012
Addenbrooke's Hospital
2012
Instituto de Química Física Blas Cabrera
2007-2011
University of Patras
2011
Novo Nordisk (Denmark)
2011
Answers from Exomes Exome sequencing, which targets only the protein-coding regions of genome, has potential to identify underlying genetic causes rare inherited diseases. Angulo et al. (p. 866 , published online 17 October; see Perspective by Conley and Fruman ) performed exome sequencing individuals seven unrelated families with severe, recurrent respiratory infections. The patients carried same mutation in gene coding for catalytic subunit phosphoinositide 3-kinase δ (PI3Kδ). caused...
Mutations in genes encoding components of the immune system cause primary immunodeficiencies. Here, we study a patient with recurrent atypical mycobacterial infection and early-onset metastatic bladder carcinoma. Exome sequencing identified two homozygous missense germline mutations, P733L P832S, JAK1 protein that mediates signalling from multiple cytokine receptors. Cells this exhibit reduced STAT phosphorylation following stimulations, induction expression interferon-regulated dysregulated...
CONTEXT AND OBJECTIVES Sickle cell disease (SCD) is the most common genetic disorder among people of African descent, affecting approximately 3,500 newborns each year in Brazil. Hydroxyurea (HU) only effective drug to treating patients with SCD, thereby reducing morbidity and mortality. The objective was analyze effects HU on SCD at our institution. DESIGN SETTING Retrospective study conducted a sickle centre Ribeirão Preto, São Paulo, METHODS We analyzed clinical laboratory data 37...
In 2010 a new Lagovirus related to rabbit haemorrhagic disease virus (RHDV) emerged in France and has since rapidly spread throughout domestic wild populations of several European countries. The virus, termed RHDV2, exhibits distinctive genetic, antigenic pathogenic features. Notably, RHDV2 kills rabbits previously vaccinated with RHDV vaccines. Here we report for the first time generation characterization RHDV2-specific virus-like particles (VLPs). Our results further confirmed differential...
p-Coumaric acid decarboxylases (PDCs) catalyze the nonoxidative decarboxylation of hydroxycinnamic acids to generate corresponding vinyl derivatives. Despite biotechnological relevance PDCs in food industry, their catalytic mechanism remains largely unknown. Here, we report insights into structural basis catalysis for homodimeric PDC from Lactobacillus plantarum (LpPDC). The global fold LpPDC is based on a flattened beta-barrel surrounding an internal cavity. Crystallographic and functional...
The hepatitis C virus NS5A protein is essential for RNA replication and virion assembly. phosphorylated on multiple residues during infections, but these sites remain uncharacterized. Here we identify serine 222 of genotype 2a as a phosphorylation site that functions negative regulator replication. This component the hyperphosphorylated form NS5A, which in good agreement with previous observations hyperphosphorylation negatively affects
Abstract Quantification of liver iron concentration (LIC) is crucial in the management patients suffering from certain pathologies that can produce overload, such as Cooley's anemia and hemochromatosis. All these must control level deposits their organs to avoid toxicity high LIC, which potentially lethal. This paper describes experimental protocols for LIC measurement using two magnetic techniques: resonance imaging (MRI) biomagnetic susceptometry (BLS). MRI proton transverse relaxation...
In this work, we analyzed at high resolution the sugar-binding mode of recombinant N-terminal ricin-B domain hemolytic protein LSLa (LSL150) from mushroom Laetiporus sulphureus and also provide functional in vitro evidence suggesting that, together with its putative receptor-binding role, module may increase solubility membrane pore-forming partner. We first demonstrate that LSL150 behaves as an autonomous folding unit active lectin. have determined crystal structure 1.47 Å...
Hidroxiureia (HU) constitui o avanço mais importante no tratamento de pacientes com doença falciforme (DF). Fortes evidências confirmam a eficácia da HU em adultos diminuindo os episódios dor intensa, hospitalização, número transfusões e síndrome torácica aguda. Embora evidência do crianças não seja tão forte, recentes resultados são encorajadores. Os dados atuais relação aos riscos curto longo prazos terapia aceitáveis comparado dos tratados HU. Neste artigo, apresentamos revisão detalhada...
O Programa de Triagem Neonatal do Hospital das Clínicas da Faculdade Medicina Ribeirão Preto, Universidade São Paulo, Brasil, instituído em 1994 diagnosticou, até 2005, 76 crianças com hipotireoidismo congênito, 10 fenilcetonúria e 25 hemoglobinopatias, o que representou uma incidência 1:2.595, 1:19.409, 1:4.120, respectivamente. Foram diagnosticadas 2.747 traço falciforme (1:37,5 nascidos vivos). A cobertura média programa foi 94,5%. Houve considerável melhora nos parâmetros avaliação...
The objective of this study was to verify the evolution pregnancies in sickle cell patients followed at one institution over a period 12 years (January 2000 June 2012). evaluated 34 pregnant women with disease mean age 23.9 ± 5.3 years. incidence obstetric complications, non-obstetric complications linked and newborn were analyzed. A total 26% cases reported previous miscarriages, 20% had preterm labor, 10% pre-eclampsia, 5% gestational diabetes. Forty-one percent deliveries cesarean...
ABSTRACT Viruses need only one or a few structural capsid proteins to build an infectious particle. This is possible through the extensive use of symmetry and conformational polymorphism proteins. Using virus-like particles (VLP) from rabbit hemorrhagic disease virus (RHDV) as model, we addressed basis calicivirus assembly their application in vaccine design. The RHDV based on T=3 lattice containing 180 identical subunits (VP1). We determined structure VLP 8.0-Å resolution by...
Abstract We report the generation, characterization and epitope mapping of a panel 26 monoclonal antibodies (MAbs) against VP1 capsid protein feline calicivirus (FCV). Two close but distinct linear epitopes were identified at outermost surface (P2 subdomain) VP1, within E5′HVR antigenic hypervariable region: one spanning amino acids 431-435 (PAGDY), highly conserved recognized by non-neutralizing MAbs; second 445-451 (ITTANQY), variable neutralizing MAbs. These might be valuable for...
ABSTRACT Noroviruses (NoVs) are responsible for the majority of gastroenteritis outbreaks in humans. Recently, NoV strains which genetically closely related to human genogroup II (GII) NoVs have been detected fecal specimens from swine. These findings raised concern about possible role pigs as reservoirs that could infect To better understand epidemiology swine both and populations, rapid immunoassays needed. In this study, baculovirus recombinants were generated express capsid gene a GII...
Intrahepatic cholestasis (SCIC) is an uncommon but potentially fatal complication of sickle cell disease (SCD), with a high death rate, observed mainly in patients homozygous anemia. Herein, we describe case severe SCIC treated successfully aggressive manual exchange transfusion (ET). The patient was admitted enlarged liver and signs hepatic failure, such as hyperbilirubinemia coagulopathy. There no evidence viral hepatitis or biliary obstruction. We performed several sessions ET order to...
Purpose Janus kinase-1 (JAK1) tyrosine kinase mediates signaling from multiple cytokine receptors, including interferon alpha/beta and gamma (IFN-α/β IFN-γ), which are important for viral mycobacterial protection respectively. We previously reported autosomal recessive (AR) hypomorphic JAK1 mutations in a patient with recurrent atypical infections relatively minor infections. This study tests the impact of partial deficiency on cellular responses to IFNs pathogen control. Methods...
Os distúrbios hereditários das hemoglobinas são as doenças genéticas mais frequentes do homem e difundidas no mundo, abrangendo sobretudo continentes como África, Américas, Europa extensas regiões da Ásia. Estima-se que haja 270 milhões de portadores hemoglobinopatias dos quais 80 talassemia. Aproximadamente 60 mil crianças nascem anualmente mundo com talassemia 250 anemia falciforme, dando uma frequência 2,4 afetadas para cada 1.000 nascimentos. No Brasil, a doenca falciforme é doença...
Virus-like particles (VLPs), comprised of viral structural proteins devoid genetic material, are tunable nanoparticles that can be chemically or genetically engineered, to used as platforms for multimeric display foreign antigens. Here, we report the engineering chimeric VLPs, derived from rabbit hemorrhagic disease virus (RHDV) presentation B-cell antigens immune system. The RHDV capsid comprises 180 copies a single subunit (VP60). To evaluate ability VLPs elicit protective humoral...
Accumulation of iron in thalassemia causes organ damage and reduces patient survival due to heart lesions the second decade life. Iron deposits are monitored by direct (biopsy) indirect methods (ferritin) with sequential data being better than isolated measurements. This paper compares two measurements overload; a single hepatic concentration (HIC) magnetic resonance mean ferritin levels over four years. A retrospective study 25 patients from Centro Regional de Hemoterapia Ribeirão Preto,...