A5078293718- Immunodeficiency and Autoimmune Disorders
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Blood disorders and treatments
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Mycobacterium research and diagnosis
- Monoclonal and Polyclonal Antibodies Research
- Animal Virus Infections Studies
- T-cell and B-cell Immunology
- Viral gastroenteritis research and epidemiology
- NF-κB Signaling Pathways
- Cytokine Signaling Pathways and Interactions
- Tuberculosis Research and Epidemiology
- Fungal Infections and Studies
- Systemic Lupus Erythematosus Research
- Adrenal Hormones and Disorders
- Cell Adhesion Molecules Research
- Respiratory viral infections research
- Immunotherapy and Immune Responses
- Inflammasome and immune disorders
- Viral Infections and Immunology Research
- Advanced Biosensing Techniques and Applications
- vaccines and immunoinformatics approaches
- Vaccine Coverage and Hesitancy
Universidad Nacional Autónoma de México
2000-2024
Universidad de Cuautitlán Izcalli
2008-2024
Addenbrooke's Hospital
2014-2022
Leeds Teaching Hospitals NHS Trust
2021
Papworth Hospital NHS Foundation Trust
2021
Cambridge University Hospitals NHS Foundation Trust
2017-2021
Wellcome Trust
2021
University of Leeds
2021
University College London
2008
Hammersmith Hospital
2000
Most patients with autoimmune polyendocrine syndrome type I (APS-I) display chronic mucocutaneous candidiasis (CMC). We hypothesized that this CMC might result from autoimmunity to interleukin (IL)-17 cytokines. found high titers of autoantibodies (auto-Abs) against IL-17A, IL-17F, and/or IL-22 in the sera all 33 tested, as detected by multiplex particle-based flow cytometry. The auto-Abs and were specific five shown Western blotting. IL-17A neutralizing only patient bioassays activity. None...
Answers from Exomes Exome sequencing, which targets only the protein-coding regions of genome, has potential to identify underlying genetic causes rare inherited diseases. Angulo et al. (p. 866 , published online 17 October; see Perspective by Conley and Fruman ) performed exome sequencing individuals seven unrelated families with severe, recurrent respiratory infections. The patients carried same mutation in gene coding for catalytic subunit phosphoinositide 3-kinase δ (PI3Kδ). caused...
Abstract SARS-CoV-2 Spike protein is critical for virus infection via engagement of ACE2, and amino acid variation in increasingly appreciated. Given both vaccines therapeutics are designed around Wuhan-1 Spike, this raises the theoretical possibility escape, particularly immunocompromised individuals where prolonged viral replication occurs. Here we report chronic with reduced sensitivity to neutralising antibodies an immune suppressed individual treated convalescent plasma, generating...
Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) transmission is uncontrolled in many parts of the world, compounded some areas by higher potential B1.1.7 variant now seen 50 countries. It unclear whether responses to SARS-CoV-2 vaccines based on prototypic strain will be impacted mutations found B.1.1.7. Here we assessed immune following vaccination with mRNA-based vaccine BNT162b2. We measured neutralising antibody a single immunization using pseudoviruses expressing wild-type...
We evaluated a patient with disseminated Mycobacterium tuberculosis and chelonae infection, of which he died. He also developed autoimmune (type I) diabetes primary hypothyroidism. His serum contained high titer immunoglobulin G autoantibody to interferon-γ (IFN-γ) capable blocking in vitro responses this cytokine by peripheral blood mononuclear cells from normal donors. These results suggest that autoantibodies IFN-γ can induce susceptibility mycobacterial may be refractory chemotherapy.
COVID-19 is associated with neurological complications including stroke, delirium and encephalitis. Furthermore, a post-viral syndrome dominated by neuropsychiatric symptoms common, seemingly unrelated to severity. The true frequency underlying mechanisms of injury are unknown, but exaggerated host inflammatory responses appear be key driver We investigated the dynamics of, relationship between, serum markers brain [neurofilament light (NfL), glial fibrillary acidic protein (GFAP) total tau]...
Complete deficiency of either the two human interferon (IFN)-γ receptor components, ligand-binding IFN-γR1 chain and signaling IFN-γR2 chain, is invariably associated with early-onset infection caused by bacille Calmette-Guérin vaccines and/or environmental nontuberculous mycobacteria, poor granuloma formation, a fatal outcome in childhood. Partial milder histopathologic clinical phenotype. Cells from 20-year-old healthy person history curable infections due to Mycobacterium abscessus mature...
Abstract Increased extracellular sodium activates Th17 cells, which provide protection from bacterial and fungal infections. Whilst high salt diets have been shown to worsen autoimmune disease, the immunological consequences of clinical depletion are unknown. Here, we investigate immunity in patients with inherited salt-losing tubulopathies (SLT). Forty-seven genotyped SLT (with Bartter, Gitelman or EAST Syndromes) recruited. Clinical features dysregulated recorded a standardised...
Abstract Two dose mRNA vaccination provides excellent protection against SARS-CoV-2. However, there are few data on vaccine efficacy in elderly individuals above the age of 80 1 . Additionally, new variants concern (VOC) with reduced sensitivity to neutralising antibodies have raised fears for vulnerable groups. Here we assessed humoral and cellular immune responses following BNT162b2 2 participants prospectively recruited from community younger health care workers. Median was 72 years 51%...
Abstract Autosomal dominant hyper-IgE syndrome caused by dominant-negative loss-of-function mutations in signal transducer and activator of transcription factor 3 ( STAT3 ) (STAT3-HIES) is a rare primary immunodeficiency with multisystem pathology. The quality life patients STAT3-HIES determined not only the progressive, life-limiting pulmonary disease, but also significant skin disease including recurrent infections abscesses requiring surgery. Our early report indicated that hematopoietic...
<title>Abstract</title> SARS-CoV-2 transmission is uncontrolled in many parts of the world, compounded some areas by higher potential B1.1.7 variant now seen 50 countries. It unclear whether responses to vaccines based on prototypic strain will be impacted mutations found B.1.1.7. Here we assessed immune following vaccination with mRNA-based vaccine BNT162b2. We measured neutralising antibody a single immunization using pseudoviruses expressing wild-type Spike protein or 8 B.1.1.7 protein....
Immune defects in chronic pulmonary aspergillosis (CPA) are poorly characterized. We compared peripheral blood cytokine profiles patients with CPA versus healthy controls and explored the relationship disease severity.Interferon-gamma (IFNγ), interleukin (IL)-17, tumor necrosis factor-α, IL-6, IL-12, IL-10 were measured after vitro stimulation of whole lipopolysaccharide (LPS), phytohemagglutinin, β-glucan, zymosan (ZYM), IL-12 or IL-18, combinations. Clinical parameters mortality correlated...
At the population level, rheumatoid arthritis (RA) is generally viewed as autoimmune in nature with a small subgroup of cases having palindromic form or systemic autoinflammatory disorder (SAID) phenotype. Herein, we describe resistant classical autoantibody associated RA that had clinical, genetic and therapeutic responses indicative coexistent disease. Five patients clinically overlapping features between SAID including polysynovitis autoantibody/shared epitope positivity, who abrupt...
Background: Vaccines remain the cornerstone for containing SARS-CoV-2 pandemic. mRNA vaccines provide protection in clinical trials using a two-dose approach, separated by three to four week gap. UK policy 2021 is extend dosing interval from twelve weeks. There paucity of data elderly, even though these individuals are first receive due risk severe disease. Here we assessed real world immune responses following vaccination with mRNA-based vaccine BNT162b2.Methods: We did prospective cohort...
Abstract Background: We developed a cost-efficient modular system for multiplex analysis of the multiple autoantibodies that characterize systemic rheumatoid diseases. Methods: The nanodot array luminometric immunoassay (NALIA) consists conventional 96-well membrane-bottomed plates in which antigens or antibodies are adsorbed onto underside membrane. Current arrays use 5 × format (25 dots/well), allows 10 analytes to be measured duplicate: double-stranded DNA (dsDNA), centromere protein B...
G6PC3 deficiency typically causes severe congenital neutropenia, associated with susceptibility to infections, cardiac and urogenital abnormalities. However, here we describe two boys of Pakistani origin who were found have due c.130 C>T mutation, but clinical phenotypes that are typical for a systemic autoinflammatory syndrome. The index case presented combination unexplained fevers, mucosal ulcers, abdominal symptoms, inflammatory arthritis. He eventually fully responded anti-TNF therapy....
Severe and disseminated non-tuberculous mycobacterial (NTM) infections are frequently linked to a genetic predisposition but acquired defects of the interferon gamma (IFNγ) / interleukin 12 (IL-12) pathway need be considered in adult patients with persistent or recurrent infections. Neutralizing anti-IFNγ autoantibodies disrupting IFNγ signalling have been identified as cause severe unique immunodeficiency syndrome increased susceptibility NTM other intracellular pathogens.An Asian female...