A5070404848- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Tuberculosis Research and Epidemiology
- Immunodeficiency and Autoimmune Disorders
- Mycobacterium research and diagnosis
- Immune responses and vaccinations
- Monoclonal and Polyclonal Antibodies Research
- Adrenal Hormones and Disorders
- Single-cell and spatial transcriptomics
- Cell Adhesion Molecules Research
- Global Public Health Policies and Epidemiology
- Nicotinic Acetylcholine Receptors Study
- Bariatric Surgery and Outcomes
- Diabetes and associated disorders
- Gallbladder and Bile Duct Disorders
- Hematopoietic Stem Cell Transplantation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Liver Disease Diagnosis and Treatment
- Dermatologic Treatments and Research
- Global Maternal and Child Health
- Advanced Biosensing Techniques and Applications
- Body Contouring and Surgery
- Lymphoma Diagnosis and Treatment
- Chronic Myeloid Leukemia Treatments
- Neonatal Respiratory Health Research
Massachusetts General Hospital
2017-2025
Ragon Institute of MGH, MIT and Harvard
2020-2025
Harvard University
2015-2025
MGH Institute of Health Professions
2023-2025
Herlev Hospital
2025
Copenhagen University Hospital
2025
Boston University
2023
Stanford University
1994-2018
Stanford Medicine
1994-2017
Brigham and Women's Hospital
2016
Patients with mutations of the recombination-activating genes (RAG) present diverse clinical phenotypes, including severe combined immune deficiency (SCID), autoimmunity, and inflammation. However, incidence extent dysregulation in RAG-dependent immunodeficiency have not been studied detail. Here, we demonstrated that patients hypomorphic RAG mutations, especially those delayed-onset granulomatous/autoimmune manifestations (CID-G/AI), produce a broad spectrum autoantibodies. Neutralizing...
Mycobacterium tuberculosis lung infection results in a complex multicellular structure: the granuloma. In some granulomas, immune activity promotes bacterial clearance, but others, bacteria persist and grow. We identified correlates of control cynomolgus macaque granulomas by co-registering longitudinal positron emission tomography computed imaging, single-cell RNA sequencing, measures clearance. Bacterial persistence occurred enriched for mast, endothelial, fibroblast, plasma cells,...
Intradermal Bacillus Calmette-Guérin (BCG) is the most widely administered vaccine, but it does not sufficiently protect adults against pulmonary tuberculosis. Recent studies in nonhuman primates show that intravenous BCG administration offers superior protection Mycobacterium tuberculosis ( Mtb ). We used single-cell analysis of bronchoalveolar lavage cells from rhesus macaques vaccinated via different routes and doses to identify alterations immune ecosystem airway following vaccination....
Autoreactivity to histones is a pervasive feature of several human autoimmune disorders, including systemic lupus erythematosus (SLE). Specific post-translational modifications (PTMs) within neutrophil extracellular traps (NETs) may potentially drive the process by which tolerance these chromatin-associated proteins broken. We hypothesized that NETs and their unique histone PTMs might be capable inducing autoantibodies target histones.We developed novel efficient method for in vitro...
The functional role of CD8+ lymphocytes in tuberculosis remains poorly understood. We depleted innate and/or adaptive macaques and showed that loss all CD8α+ cells (using anti-CD8α antibody) significantly impaired early control Mycobacterium (Mtb) infection, leading to increased granulomas, lung inflammation, bacterial burden. Analysis barcoded Mtb from infected demonstrated depletion allowed establishment lungs dissemination within lymph nodes, while only T (with anti-CD8β worsened nodes....
Abstract Intradermal (ID) Bacillus Calmette–Guérin (BCG) is the most widely administered vaccine in world. However, ID-BCG fails to achieve level of protection needed adults alter course tuberculosis epidemic. Recent studies non-human primates have demonstrated high levels against Mycobacterium ( Mtb ) following intravenous (IV) administration BCG. protective immune features that emerge IV BCG vaccination remain incompletely defined. Here we used single-cell RNA-sequencing (scRNAseq)...
Effective therapeutic vaccines often require activation of T cell-mediated immunity. Robust cell activation, including CD8 responses, can be achieved using antibodies or antibody fragments to direct antigens interest professional antigen presenting cells. This approach represents an important advance in enhancing vaccine efficacy. Nucleic acid aptamers present a promising alternative protein-based targeting approaches. We have selected that specifically bind the murine receptor, DEC205,...
Anti-cytokine autoantibodies (ACAAs) have been described in a growing number of primary immunodeficiencies with autoimmune features including Autoimmune Polyendocrine Syndrome Type I (APS-1), prototypical disease defective T cell central tolerance. Whether defects peripheral tolerance lead to similar ACAAs is unknown. Immunodysregulation Polyendocrinopathy Enteropathy X-linked (IPEX) caused by mutations FOXP3, master regulator regulatory cells (Treg), and consequently results Unique...
Introduction:Portal vein thrombosis has been documented after laparoscopic general surgery and uncommonly observed bariatric surgery. Among operations, the sleeve gastrectomy is being performed with ever-increasing frequency. Here we report case of a man who presented portal gastrectomy.Case Description:A 41-y-old underwent an uneventful for treatment morbid obesity, on postoperative day 10 nonfocal abdominal pain, nausea, vomiting, leukocytosis. Computed tomography revealed thrombosis,...
Tuberculosis (TB) remains a major global health challenge, particularly in the context of multidrug-resistant (MDR) Mycobacterium tuberculosis (Mtb). Host-directed therapies (HDTs) have been proposed as adjunctive therapy to enhance immune control infection. Recently, one such HDT, pharmacologic modulation myeloid-derived suppressor cells (MDSCs), has treat MDR-TB. While MDSCs well characterized cancer, their role TB pathogenesis unclear. To investigate whether or other myeloid populations...
INTRODUCTION. Annually, more than 9,000 cholecystectomies are performed in Denmark. All gallbladders submitted for histological examination to rule out preoperatively undetected gallbladder cancer. The at the Department of Pathology is costly. Therefore, it relevant investigate whether adopting a selective approach which only selected reasonable. METHODS. Herlev Hospital from 1 January 2014 31 December 2023 were included study. In cases cancer, results preoperative diagnostic imaging,...
Abstract Mycobacterium tuberculosis lung infection results in a complex multicellular structure, the granuloma. In some granulomas, immune activity promotes bacterial clearance; others, bacteria persist and grow. We identified correlates of control cynomolgus macaque granulomas by co-registering longitudinal PET-CT imaging, single-cell RNA-sequencing, measures clearance. find that persistence occurs enriched for mast, endothelial, fibroblast plasma cells, signaling amongst themselves via...