A5054556546- Extracellular vesicles in disease
- Bone health and treatments
- Ferroptosis and cancer prognosis
- MicroRNA in disease regulation
- Calcium signaling and nucleotide metabolism
- Advanced Proteomics Techniques and Applications
- Inhalation and Respiratory Drug Delivery
- Genital Health and Disease
- Surgical Sutures and Adhesives
- Galectins and Cancer Biology
- Proteoglycans and glycosaminoglycans research
- Mass Spectrometry Techniques and Applications
- Protist diversity and phylogeny
- Orthopedic Surgery and Rehabilitation
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cell Adhesion Molecules Research
- Facial Trauma and Fracture Management
- Cancer Diagnosis and Treatment
- Nanopore and Nanochannel Transport Studies
- Carbohydrate Chemistry and Synthesis
- Lysosomal Storage Disorders Research
- Urologic and reproductive health conditions
- Bladder and Urothelial Cancer Treatments
- Fibroblast Growth Factor Research
Cardiff Metropolitan University
2016-2019
Velindre Cancer Centre
2009-2017
Cardiff University
2009-2017
University Hospital of Wales
2017
AO Foundation
2012
ABSTRACT Bioinformatics tools are imperative for the in depth analysis of heterogeneous high‐throughput data. Most software developed by specific laboratories or groups companies wherein they designed to perform required group. However, such may fail capture “what community needs a tool”. Here, we describe novel community‐driven approach build comprehensive functional enrichment tool. Using existing FunRich tool as template, invited researchers request additional features and/or changes....
Abstract Background Recently, nanometer sized vesicles (termed exosomes) have been described as a component of urine. Such may be useful non-invasive source markers in renal disease. Their utility urological cancer remains unstudied. Our aim this study was to investigate the feasibility and value analysing urinary exosomes prostate patients undergoing standard therapy. Methods Ten (with locally advanced PCa) provided spot urine specimens at three time points during Patients received 3–6...
Proteomic studies of circulating vesicles are hampered by difficulties in purifying from plasma and serum. Isolations contaminated with high-abundance blood proteins that may mask genuine vesicular-associated and/or simply provide misleading data. In this brief report, we explored the potential utility a commercially available size exclusion chromatography column for rapid vesicle purification. We evaluated performance column, cancer cell line conditioned medium or healthy donor plasma,...
The discovery of disease biomarkers, along with the use "liquid biopsies" as a minimally invasive source continues to be great interest. In inflammatory diseases central nervous system (CNS), cerebrospinal fluid (CSF) is most obvious biofluid source. Extracellular vesicles (EVs) are also present in CSF and thought potential "biomarker treasure chests". However, isolating these CSF-derived EVs remains challenging. This small-scale pilot study developed tested protocol enrich for CSF-EVs, both...
Proteomics analysis of biofluid-derived vesicles holds enormous potential for discovering non-invasive disease markers. Obtaining sufficient quality and quantity profiling studies has, however, been a major problem, as samples are often replete with co-isolated material that can interfere the identification genuine low abundance, vesicle components. Here, we used combination ultracentrifugation size-exclusion chromatography to isolate analyse plasma or urine origin. We describe...
Aminobisphosphonates (NBPs) are used widely against excessive bone resorption in osteoporosis and Paget's disease as well metastatic multiple myeloma. Intravenous NBP administration often causes mild to severe acute-phase responses (APRs) that may require intervention with analgesics antipyretics lead treatment noncompliance nonadherence. We here undertook a phase IV safety trial patients investigate the APR of otherwise healthy individuals first-time intravenous zoledronate. This study...
Abstract Soft tissue‐implant adhesion is often required for implant integration into the body; however, in some situations, tissue to glide freely over an implant. In case of distal radius fracture treatment, current literature describes how titanium and its alloys tend lead more intra‐tendon inflammatory reactions compared with stainless steel. This leads tendon‐implant damage possibly causing limited palmar flexion even tendon rupture. The goal this study was analyze effect different...
Abstract Niemann-Pick disease type C1 (NPC disease) is a neurodegenerative multi-lipid lysosomal storage caused by mutations in the NPC1 gene presenting with reduced Ca 2+ signalling and inhibited late endosome-lysosome transport. Elevating cytosolic levels NPC cells has been shown to reduce lipid storage. Treating Npc1 -/- mice modulator curcumin led storage, improved life expectancy function. These studies reported utilisation of supplements families despite there being no clinical...