A5041682013- Advanced Proteomics Techniques and Applications
- Prostate Cancer Treatment and Research
- RNA modifications and cancer
- Mass Spectrometry Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Extracellular vesicles in disease
- Glioma Diagnosis and Treatment
- Radiomics and Machine Learning in Medical Imaging
- Mitochondrial Function and Pathology
- Ferroptosis and cancer prognosis
- Molecular Biology Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Cancer, Lipids, and Metabolism
- Ubiquitin and proteasome pathways
- Advanced Glycation End Products research
- Cancer Genomics and Diagnostics
- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- Neuroblastoma Research and Treatments
- Cancer Cells and Metastasis
- Glycosylation and Glycoproteins Research
- RNA and protein synthesis mechanisms
- Machine Learning in Bioinformatics
- RNA Research and Splicing
- Cancer Research and Treatments
Princess Margaret Cancer Centre
2016-2025
University Health Network
2016-2025
University of Toronto
2016-2025
McMaster University
2023-2024
Ontario Institute for Cancer Research
2007-2023
Health Net
2022-2023
National Research Council Canada
2022
International Federation on Ageing
2021
Jagiellonian University
2014
University Medical Center
2014
Recent studies suggested that interruption of the interaction advanced glycation end products (AGEs), with signal-transducing receptor for AGE (RAGE), by administration soluble, extracellular ligand-binding domain RAGE, reversed vascular hyperpermeability and suppressed accelerated atherosclerosis in diabetic rodents. Since precise molecular target soluble RAGE those settings was not elucidated, we tested hypothesis predominant specific AGEs within tissues disorders such as diabetes renal...
Previous studies suggested that blockade of RAGE in diabetic apolipoprotein (apo) E-null mice suppressed early acceleration atherosclerosis. A critical test the potential applicability to clinical settings was its ability impact established vascular disease. In this study, we tested hypothesis contributed lesion progression atherosclerosis apoE-null mice.Male mice, age 6 weeks, were rendered with streptozotocin or treated citrate buffer. At 14 certain killed once-daily murine soluble...
Background — The products of nonenzymatic glycation and oxidation proteins, the advanced end (AGEs), form under diverse circumstances such as aging, diabetes, kidney failure. Recent studies suggested that AGEs may in inflamed foci, driven by or myeloperoxidase pathway. A principal means which alter cellular properties is through interaction with their signal-transduction receptor RAGE. We tested hypothesis RAGE on endothelial cells enhances vascular activation. Methods Results AGEs, RAGE,...
Abstract. Advanced glycation end products (AGE) contribute to diabetic tissue injury by two major mechanisms, i.e. , the alteration of extracellular matrix architecture through nonenzymatic glycation, with formation protein crosslinks, and modulation cellular functions interactions specific cell surface receptors, best characterized which is receptor for AGE (RAGE). Recent evidence suggests that AGE-RAGE interaction may also be promoted inflammatory processes oxidative injury. To...
Abstract Motivation: Extracellular vesicles (EVs) are spherical bilayered proteolipids, harboring various bioactive molecules. Due to the complexity of vesicular nomenclatures and components, online searches for EV-related publications components currently challenging. Results: We present an improved version EVpedia, a public database EVs research. This community web portal contains identification orthologous bioinformatic tools personalized function. EVpedia includes 6879 publications, 172...
ABSTRACT Bioinformatics tools are imperative for the in depth analysis of heterogeneous high‐throughput data. Most software developed by specific laboratories or groups companies wherein they designed to perform required group. However, such may fail capture “what community needs a tool”. Here, we describe novel community‐driven approach build comprehensive functional enrichment tool. Using existing FunRich tool as template, invited researchers request additional features and/or changes....
Intratumoral heterogeneity is a critical frontier in understanding how the tumor microenvironment (TME) propels malignant progression. Here, we deconvolute human pancreatic TME through large-scale integration of histology-guided regional multiOMICs with clinical data and patient-derived preclinical models. We discover "subTMEs," histologically definable tissue states anchored fibroblast plasticity, relationships to immunity, subtypes, differentiation, treatment response. "Reactive" subTMEs...
Diabetes is associated with increased prevalence, severity, and progression of periodontal disease. To test the hypothesis that activation RAGE (Receptor for Advanced Glycation End products) contributes to pathogenesis diabetes-associated periodontitis, we treated diabetic mice, infected human pathogen Porphyromonas gingivalis, soluble (sRAGE). sRAGE extracellular domain receptor, which binds ligand blocks interaction with, of, cell-surface RAGE. Blockade diminished alveolar bone loss in a...
Abstract —Advanced glycation end products (AGEs) and their cell surface receptor, RAGE, have been implicated in the pathogenesis of diabetic complications. Here, we studied role RAGE expression its proinflammatory ligands, EN-RAGEs (S100/calgranulins), inflammatory events mediating cellular activation tissue. Apolipoprotein E–null mice were rendered with streptozotocin at 6 weeks age. Compared nondiabetic aortas kidneys, kidneys displayed increased EN-RAGEs, 2 key markers vascular...
Article16 June 2009Open Access Comparative systems biology of human and mouse as a tool to guide the modeling placental pathology Brian Cox Corresponding Author The Hospital for Sick Children, Program in Developmental Stem Cell Biology, Toronto, Ontario, Canada Search more papers by this author Max Kotlyar Division Signaling Ontario Cancer Institute, Department Medical Biophysics, University Andreas I Evangelou Genomics Proteomics, Vladimir Ignatchenko Alex Kathie Whiteley Samuel Lunenfeld...
Epithelial ovarian cancer is the most lethal gynecological malignancy, and disease-specific biomarkers are urgently needed to improve diagnosis, prognosis, predict monitor treatment efficiency. We present an in-depth proteomic analysis of selected biochemical fractions human ascites, resulting in stringent confident identification over 2500 proteins. Rigorous filter schemes were applied objectively minimize number false-positive identifications, we only report proteins with substantial...
Expressed prostatic secretions ( EPS ) are proximal fluids of the prostate that increasingly being utilized as a clinical source for diagnostic and prognostic assays cancer PC a). These contain an abundant amount microvesicles reflecting secretory function gland, their protein composition remains poorly defined in relation to a. Using expressed urine ‐urine), exosome preparations were characterized by shotgun proteomics procedure. In pooled ‐urine samples, ∼900 proteins detected. Many these...