A5004512548- Prostate Cancer Treatment and Research
- Molecular Biology Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Prostate Cancer Diagnosis and Treatment
- Glycosylation and Glycoproteins Research
- Cancer, Lipids, and Metabolism
- Protein purification and stability
- Mass Spectrometry Techniques and Applications
- Peptidase Inhibition and Analysis
- Health Systems, Economic Evaluations, Quality of Life
- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
- Hormonal and reproductive studies
- Cancer-related molecular mechanisms research
- Ubiquitin and proteasome pathways
- Fatty Acid Research and Health
- Statistical Methods in Clinical Trials
- Urologic and reproductive health conditions
- Cell Adhesion Molecules Research
- S100 Proteins and Annexins
- Metabolomics and Mass Spectrometry Studies
- PI3K/AKT/mTOR signaling in cancer
- Bladder and Urothelial Cancer Treatments
- Cancer Research and Treatments
- Cancer, Hypoxia, and Metabolism
Eastern Virginia Medical School
2014-2024
The University of Texas Health Science Center at San Antonio
2009-2024
The University of Texas Health Science Center at Houston
1996-2024
Cancer Research Center
2011-2024
The University of Texas at San Antonio
1987-2024
Sentara Norfolk General Hospital
2013-2020
Cleveland Clinic
2016
University of Fairfax
2015
Lerøy (Norway)
2013
Urology of Virginia
2009-2012
Extraprostatic disease will be manifest in a third of men after radical prostatectomy. We present the long-term followup randomized clinical trial radiotherapy to reduce risk subsequent metastatic and death.A total 431 with pT3N0M0 prostate cancer were 60 64 Gy adjuvant or observation. The primary study end point was metastasis-free survival.Of 425 eligible 211 observation 214 radiation. Of those under 70 ultimately received radiotherapy. Metastasis-free survival significantly greater (93...
ContextDespite a stage-shift to earlier cancer stages and lower tumor volumes for prostate cancer, pathologically advanced disease is detected at radical prostatectomy in 38% 52% of patients. However, the optimal management these patients after unknown.ObjectiveTo determine whether adjuvant radiotherapy improves metastasis-free survival with stage pT3 N0 M0 cancer.Design, Setting, PatientsRandomized, prospective, multi-institutional, US clinical trial enrollment between August 15, 1988,...
A recently developed matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) method to spatially profile the location and distribution of multiple N-linked glycan species in frozen tissues has been extended improved for direct analysis glycans clinically derived formalin-fixed paraffin-embedded (FFPE) tissues. Formalin-fixed from normal mouse kidney, human pancreatic prostate cancers, a hepatocellular carcinoma tissue microarray were processed by antigen retrieval...
The DOCUMENT multicenter trial in the United States validated performance of an epigenetic test as independent predictor prostate cancer risk to guide decision making for repeat biopsy. Confirming increased negative predictive value could help avoid unnecessary biopsies.We evaluated archived, biopsy core tissue samples 350 subjects from a total 5 urological centers States. All underwent within 24 months with (controls) or positive (cases) histopathological result. Centralized blinded...
Abstract We previously showed, by immunohistochemistry with phospho-specific antibodies, increased phosphorylation (activation) of Akt (Ser473) [phosphorylated (pAkt)] in high-Gleason grade prostate cancer (Malik SN, et al., Clin Cancer Res 2002;8:1168–71). Elevation pAkt was accompanied decreased extracellular signal-regulated kinase (ERK) 1/2 (Thr202/Tyr204) ERK (pERK)], indicative inactivation. In this report, we determined whether and pERK predicted clinical outcome. Prostate-specific...
Abstract BACKGROUND Recent studies of men with prostate carcinoma suggest that obesity may be associated more advanced‐stage disease and lower overall survival rates. One possible link between body mass index (BMI) prognosis ascertainment. Prostate‐specific antigen (PSA) is widely used to screen for carcinoma. METHODS The authors examined the association BMI PSA in a population‐based study 2779 without Between 2001 2004, these were enrolled sponsored by San Antonio Center Biomarkers Risk,...
We showed previously that glomerular mesangial cells displayed increased fibronectin, laminin, and type IV collagen synthesis mRNA levels when grown in medium containing 30 mM glucose compared with those 10 [S. H. Ayo, R. A. Radnik, W. F. Glass II, J. Garoni, E. Rampt, D. Appling, I. Kreisberg. Am. Physiol. 260 (Renal Fluid Electrolyte 29): F185-F191, 1990]. However, total protein actin were unchanged. In this report, we show an increase concentration resulted diacylglycerol (DAG) mass...
To analyse primary haemostasis in the zebrafish we have identified and characterized thrombocyte by morphologic, immunologic functional approaches. Novel methods were developed for harvesting blood with preservation of thrombocytes, assaying whole adhesion/aggregation responses microtitre plates. Light electron microscopy illustrated morphological characteristics including formation aggregates, pseudopodia, surface‐connected vesicles analagous to platelet canalicular system. Immunostaining...
Histopathology is the standard approach for tissue diagnostics and centerpiece of pathology. Although current system provides prognostic information, there need molecular markers that enhance diagnosis better predict clinical prognosis. The ability to localize disease-specific changes in biopsy would help improve critical pathology decision making. Direct profiling proteins from using matrix-assisted laser desorption/ionization imaging mass spectrometry has potential supplement morphology...
Expressed prostatic secretions ( EPS ) are proximal fluids of the prostate that increasingly being utilized as a clinical source for diagnostic and prognostic assays cancer PC a). These contain an abundant amount microvesicles reflecting secretory function gland, their protein composition remains poorly defined in relation to a. Using expressed urine ‐urine), exosome preparations were characterized by shotgun proteomics procedure. In pooled ‐urine samples, ∼900 proteins detected. Many these...
No AccessJournal of UrologyAdult Urology1 Feb 2016Outcomes Active Surveillance for Clinically Localized Prostate Cancer in the Prospective, Multi-Institutional Canary PASS Cohort Lisa F. Newcomb, Ian M. Thompson, Hilary D. Boyer, James Brooks, Peter R. Carroll, Matthew Cooperberg, Atreya Dash, William J. Ellis, Ladan Fazli, Ziding Feng, Martin E. Gleave, Priya Kunju, Raymond S. Lance, Jesse K. McKenney, Maxwell V. Meng, Marlo Nicolas, G. Sanda, Jeffry Simko, Alan So, Maria Tretiakova, Dean...
Clinical observations and mouse models have suggested that inflammation can be pro-tumorigenic. Since chemokines are critical in leukocyte trafficking, we hypothesized play essential roles inflammation-associated cancers. Screening for 37 prostate cancer cell lines xenografts revealed CXCL16, the ligand receptor CXCR6, as most consistently expressed chemokine. Immunohistochemistry and/or immunofluorescence confocal imaging of 121 human specimens showed CXCL16 CXCR6 were co-expressed, both on...
Histologic grading remains the gold standard for prognosis in prostate cancer, and assessment of Gleason score plays a critical role active surveillance management. We sought to optimize prognostic stratification developed method recording studying individual architectural patterns by light microscopic evaluation that is independent grade. Some evaluated are not assessed current (eg, reactive stromal response). Individual histologic were correlated with recurrence-free survival retrospective...
Abstract Biomarkers are rapidly gaining importance in personalized medicine. Although numerous molecular signatures have been developed over the past decade, there is a lack of overlap and many biomarkers fail to validate independent patient cohorts hence not useful for clinical application. For these reasons, identification novel robust remains formidable challenge. We combine targeted proteomics with computational biology discover proteomic prostate cancer. Quantitative conducted expressed...
Aberrant glycosylation is a universal feature of cancer cells, and cancer-associated glycans have been detected in virtually every type. A common change tumour cell an increase α2,6 sialylation N-glycans, modification driven by the sialyltransferase ST6GAL1. ST6GAL1 overexpressed numerous types, sialylated are fundamental for growth, metastasis, immune evasion, drug resistance, but role prostate poorly understood. Here, we analyse matched normal tissue samples from 200 patients verify that...
The farnesyltransferase inhibitor L-744,832 selectively blocks the transformed phenotype of cultured cells expressing a mutated H-ras gene and induces dramatic regression mammary salivary carcinomas in mouse tumor virus (MMTV)-v-Ha-ras transgenic mice. To better understand how inhibitors might be used treatment human tumors, we have further explored mechanisms by which variety models. We assessed whether apoptosis or alterations cell cycle distribution found that MMTV-v-Ha-ras mice could...
In the prostate, conversion of testosterone to dihydrotestosterone (DHT), by enzymes 5alpha-reductase types 1 and 2 (5alphaR1, 5alphaR2) is required for normal growth probably also development prostate cancer (PCa). Finasteride, a 5alphaR2 inhibitor, was shown reduce prevalence PCa in Prostate Cancer Prevention Trial. However, inhibition both 5alphaR isoenzymes causes greater decrease serum DHT. The aim this study assess differential expression these at various stages...
Abstract Purpose: A considerable fraction of patients who undergo radical prostatectomy as treatment for primary prostate cancer experience biochemical recurrence detected by elevated serum levels prostate-specific antigen. In this study, we investigate whether loss expression the tumor suppressor phosphatase and tensin homologue deleted on chromosome 10 (PTEN) phosphorylated form cell survival protein Akt (pAkt) predicts recurrence. Experimental Design: Expression PTEN pAkt was...
Abstract Background: The purpose of this investigation was to examine the association obesity and adipokines leptin, adiponectin, interleukin-6 (IL-6) with prostate cancer risk aggressiveness. Methods: One hundred twenty-five incident cases 125 age-matched controls were sampled from among participants in original San Antonio Center for Biomarkers Risk Prostate Cancer cohort study. odds ratios (OR) high-grade disease (Gleason sum >7) associated WHO categories body mass index (kg/m2)...
The analysis of bodily fluids using SELDI-TOF MS has been reported to identify signatures spectral peaks that can be used differentiate patients with a specific disease from normal or control patients. This report is the 2nd 2 companion articles describing validation study approach IMAC surface sample processing prostatic adenocarcinoma.We sought derive decision algorithm for classification prostate cancer data new retrospective cohort 400 specimens. was selected minimize possible...
The glomerular mesangium is composed of matrix material and at least two cell types. One a bone marrow-derived phagocyte the other smooth muscle-like cell. phagocytic represents approximately 3-7% total mesangial population. other, more abundant, type appears to be contractile therefore has been proposed play role in regulating surface area for filtration, one component ultrafiltration coefficient, Kf. In this review we discuss properties cultured cells as well phenotypic alterations that...
This report and a companion describe validation of the ability serum proteomic profiling via SELDI-TOF mass spectrometry to detect prostatic cancer. Details this 3-stage process have been described. describes development algorithm results blinded test for stage 1.We derived decision used in study from analysis samples patients with prostate cancer (n = 181) benign hyperplasia (BPH) 143) normal controls 220). We also set separate, geographically diverse 42 without Aliquots were subjected...