A5100718304- Extracellular vesicles in disease
- Advanced Memory and Neural Computing
- MicroRNA in disease regulation
- Ferroelectric and Negative Capacitance Devices
- RNA Interference and Gene Delivery
- Neuroscience and Neural Engineering
- Semiconductor materials and devices
- 3D Printing in Biomedical Research
- Transition Metal Oxide Nanomaterials
- thermodynamics and calorimetric analyses
- Advanced biosensing and bioanalysis techniques
- Advanced MEMS and NEMS Technologies
- Viral Infections and Vectors
- Bacterial Infections and Vaccines
- Tissue Engineering and Regenerative Medicine
- Nanopore and Nanochannel Transport Studies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microfluidic and Bio-sensing Technologies
- Bone Tissue Engineering Materials
- Nanoplatforms for cancer theranostics
- Liver physiology and pathology
- Additive Manufacturing and 3D Printing Technologies
- Fluid Dynamics and Thin Films
- Sensor Technology and Measurement Systems
- Thermal properties of materials
Seoul St. Mary's Hospital
2025
Catholic University of Korea
2025
University of Michigan–Ann Arbor
2015-2024
Pohang University of Science and Technology
2015-2024
Sogang University
2023
Samsung Medical Center
2020
Sungkyunkwan University
2020
Michigan United
2019
Korea Post
2011-2019
Government of the Republic of Korea
2019
Exosomes, the endogenous nanocarriers that can deliver biological information between cells, were recently introduced as new kind of drug delivery system. However, mammalian cells release relatively low quantities exosomes, and purification exosomes is difficult. Here, we developed bioinspired exosome-mimetic nanovesicles chemotherapeutics to tumor tissue after systemic administration. The chemotherapeutics-loaded produced by breakdown monocytes or macrophages using a serial extrusion...
Graphene-substrate-promoted human neural stem cell adhesion and its differentiation into neurons is reported. Microarray studies were performed to explore plausible explanation for this effect. Further, an electrical stimulation on differentiated cells via graphene electrodes demonstrated. Detailed facts of importance specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset. They made available submitted by the authors. Please...
We analyze the response of identical pulses on a filamentary resistive memory (RRAM) to implement synapse function in neuromorphic systems. Our findings show that multilevel states conductance are achieved by varying measurement conditions related formation and rupture conductive filament. Furthermore, abrupt set switching behavior RRAM leads an unchanged state, leading degradation accuracy pattern recognition. Thus, we demonstrate linear potentiation (or depression) under using effect...
Abstract Motivation: Extracellular vesicles (EVs) are spherical bilayered proteolipids, harboring various bioactive molecules. Due to the complexity of vesicular nomenclatures and components, online searches for EV-related publications components currently challenging. Results: We present an improved version EVpedia, a public database EVs research. This community web portal contains identification orthologous bioinformatic tools personalized function. EVpedia includes 6879 publications, 172...
Extracellular vesicles are released by various cell types, particularly tumor cells, and may be potential targets for blood-based cancer diagnosis. However, studies performed on blood-borne to date have been limited lack of effective, standardized purification strategies. Using in situ prepared nanoporous membranes, we present a simple strategy employing microfluidic filtration system isolate from whole blood samples. This method can applied purify nano-sized particles allowing isolation...
Biosensors, based on graphene-encapsulated nanoparticle arrays, for highly sensitive and selective detection of breast cancer biomarkers are successfully demonstrated. The increased surface-to-volume ratio significantly helped in lowering the limits (1 pM) target biomarkers. In addition, nature biosensor is demonstrated by detecting low concentrations Detailed facts importance to specialist readers published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or...
Natural biomaterials such as hyaluronic acid, gelatin and collagen provide excellent environments for tissue regeneration. Furthermore, gel-state natural are advantageous encapsulating cells growth factors. In cell printing technology, hydrogel which contains was printed directly to form three-dimensional (3D) structures or organ regeneration using various types of printers. However, maintaining the 3D shape structure, is made only hydrogel, very difficult due its weak mechanical properties....
Several studies have focused on the regeneration of liver tissue in a two-dimensional (2D) planar environment, whereas actual is three-dimensional (3D). Cell printing technology has been successfully utilized for building 3D structures; however, poor mechanical properties cell-laden hydrogels are major concern. Here, we demonstrate construct and its application to engineering using cell through multi-head tissue/organ system. Polycaprolactone (PCL) was used as framework material because...
The presence of malignant ascites in the peritoneal cavity is a poor prognostic indicator low survival rate. Various cancer cells, including those colorectal (CRC), release microvesicles (exosomes) into surrounding tissues and peripheral circulation ascites. Although recent progress has revealed that play multiple roles tumor progression, protein composition pathological function ascites-derived are still unknown. Here, we report first global proteomic analyses highly purified derived from...
Evaluation of kinetic distribution and behaviors nanoparticles in vivo provides crucial clues into their roles living organisms. Extracellular vesicles are evolutionary conserved nanoparticles, known to play important biological functions intercellular, inter-species, inter-kingdom communication. In this study, the first analysis biodistribution outer membrane (OMVs)—bacterial extracellular vesicles—with immune-modulatory is performed. OMVs, injected intraperitoneally, spread whole mouse...
Abstract Extracellular vesicles (EVs) such as exosomes and microvesicles released from cells are potential biomarkers for blood-based diagnostic applications. To exploit EVs biomarkers, an effective pre-analytical process is necessary. However, recent studies performed with blood-borne have been hindered by the lack of purification strategies. In this study, efficient EV isolation method was developed using polyethylene glycol/dextran aqueous two phase system (ATPS). This provides high...
Multilevel cell (MLC) storage technology is attractive in achieving ultrahigh density memory with low cost. In this letter, we have demonstrated 3-bit per characteristics a TaO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">x</sub> -based RRAM. By analyzing the key requirements for MLC operation mainly switching uniformity and stability of resistance levels, an engineered stack based on thermodynamics top electrode/(vacancy reservoir/defect...
We propose a microfluidic system that generates nanovesicles (NVs) by slicing living cell membrane with microfabricated 500 nm-thick silicon nitride (SixNy) blades. Living cells were sliced the blades while flowing through microchannels lined Plasma fragments from self-assembled into spherical NVs of ~100-300 nm in diameter. During self-assembly, plasma enveloped exogenous materials (here, polystyrene latex beads) buffer solution. About 30% beads encapsulated NVs, and generated delivered...
Abstract Extracellular vesicles (EVs) may play an important role in cancer development and progression. We aimed to investigate the prognostic potential of prostate-specific EVs prostate (PCa) patients. Plasma tissue were collected from patients who underwent surgery for PCa (n = 82) or benign prostatic hyperplasia (BPH, n 28). To analyze quantity prostate, we performed transmission electron microscopy (TEM), immuno-TEM with CD63 membrane antigen (PSMA), immunofluorescence staining. After EV...
Abstract Extracellular vesicles (EVs) are secreted nano‐sized that contain cellular proteins, lipids, and nucleic acids. Although EVs expected to be biologically diverse, current analyses cannot adequately characterize this diversity because most ensemble methods inevitably average out information from diverse EVs. Here we describe a single vesicle analysis, which directly visualizes marker expressions of individual using total internal‐reflection microscopy analyzes their co‐localization...
Although urinary exosomal microRNAs (miRNAs) have recently emerged as potential biomarkers, clinical applications are still limited due to their low concentration in small volumes of samples. Therefore, the development a non-invasive, specific diagnostic tool, along with profiling miRNA markers from urine, remains significant challenge. Here, we present hydrogel-based hybridization chain reaction (HCR) for multiplex signal amplification detect miRNAs human We succeeded identifying amounts...
Abstract An extracorporeal bioartificial liver device has the potential to provide temporary hepatic support for patients with failure. Our goal was optimize flow environment cultured hepatocytes in a flat‐plate bioreactor, specifically focusing on oxygen delivery using high medium rates while reducing detrimental effects of resulting shear stresses. We used photolithographic techniques fabricate microgrooves onto underlying glass substrate. The microgrooves, perpendicular axial direction,...
The notion that widespread infectious diseases could be best managed by developing potent, adjuvant-free vaccines has resulted in the use of various biological immune-stimulating components as new vaccine candidates. Recently, extracellular vesicles, also known exosomes and microvesicles mammalian cells outer membrane vesicles Gram-negative bacteria, have gained attention for next generation vaccine. However, more invasive effective is delivery, risk it holds severe immune toxicity. Here,...
Extracellular vesicles (EVs) are small membrane-bound bodies that released into extracellular space by diverse cells, and found in body fluids like blood, urine saliva. EVs contain RNA, DNA proteins, which can be biomarkers for diagnosis. obtained minimally-invasive biopsy, so they useful disease High yield purity contribute to precise diagnosis of disease, but damaged impurities cause confu sed results. However, EV isolation methods have different yields purities. Furthermore, the method is...
We present a simple and rapid method to isolate extracellular vesicles (EVs) by using polyethylene glycol/dextran aqueous two-phase system (ATPS). This isolated more than ~75% of melanoma-derived EVs from mixture serum proteins. To increase the purity EVs, batch procedure was combined as additional steps remove protein contaminants, removed ~95% contaminants. also performed RT-PCR western blotting verify diagnostic applicability detected mRNA derived melanoma cells CD81 in EVs.