A5064542717- Extracellular vesicles in disease
- Immune cells in cancer
- MicroRNA in disease regulation
- Immune Cell Function and Interaction
- Chemokine receptors and signaling
- RNA Interference and Gene Delivery
- Cell Adhesion Molecules Research
- Platelet Disorders and Treatments
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Proteoglycans and glycosaminoglycans research
- Hematopoietic Stem Cell Transplantation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Inflammatory Biomarkers in Disease Prognosis
- Immune Response and Inflammation
- Mesenchymal stem cell research
- Phytochemicals and Antioxidant Activities
- Botanical Research and Applications
- Phagocytosis and Immune Regulation
- Cancer Cells and Metastasis
- Viral Infections and Vectors
- Cancer-related molecular mechanisms research
- Cancer Research and Treatments
- Immunodeficiency and Autoimmune Disorders
- Nanopore and Nanochannel Transport Studies
Jagiellonian University
2015-2024
Uniwersytecki Szpital Dziecięcy
2024
Cracow University of Technology
2024
University Medical Center
2014
Pohang University of Science and Technology
2014
AGH University of Krakow
2014
University of Louisville
2001-2002
Canadian Blood Services
2001
University of Alberta
2001
University of Pennsylvania
2001
Abstract Motivation: Extracellular vesicles (EVs) are spherical bilayered proteolipids, harboring various bioactive molecules. Due to the complexity of vesicular nomenclatures and components, online searches for EV-related publications components currently challenging. Results: We present an improved version EVpedia, a public database EVs research. This community web portal contains identification orthologous bioinformatic tools personalized function. EVpedia includes 6879 publications, 172...
To better define the role HIV-related chemokine receptor-chemokine axes play in human hematopoiesis, we investigated function of CXCR4 and CCR5 receptors myeloid, T- B-lymphoid cell lines selected for expression these (CXCR4+, CXCR4+ CCR5+, CCR5+ lines). We evaluated phosphorylation MAPK p42/44, AKT, STAT proteins examined ability ligands (stromal-derived factor-1 [SDF-1] macrophage inflammatory protein-1β [MIP-1β]) to influence growth, apoptosis, adhesion, production vascular endothelial...
WNT signaling plays an important role in fibrotic processes the heart. Recently, exosomes have been proposed as novel extracellular transporters for proteins. In this study, we analyzed whether WNT3a and WNT5a carried by could activate downstream molecular pathways human cardiac fibroblasts. Exosomes were isolated from conditioned medium of control, WNT3a- WNT5a-producing L cells differential ultracentrifugations. Obtained showed size ranging between 20⁻150 nm expressed exosomal markers...
Atherosclerosis, a common age-related disease, is characterized by intense immunological activity. Atherosclerotic plaque composed of endothelial cells, vascular smooth muscle cells (VSMCs), lipids and immune infiltrating from the blood. During progression VSMCs undergo senescence within secrete SASP (senescence-associated secretory phenotype) factors that can actively modulate microenvironment. We demonstrated senescent increased number extracellular vesicles (senEVs). Based on unbiased...
Natural killer (NK) cells plays a pivotal role in the control of viral infections, and their function depend on balance between activating inhibitory receptors. The immune dysregulation observed COVID-19 patients was previously associated with downregulation NK cell numbers function, yet mechanism inhibition functions interplay infected remain largely unknown. In this study we show that SARS-CoV-2 infection airway epithelial can directly influence phenotype microenvironment. were co-cultured...
Tumour-derived microvesicles (TMVs) are important players in tumour progression, modulating biological activity of immune cells e.g. lymphocytes, monocytes and macrophages. This phenomenon is particularly interesting the progression colon cancer, as macrophages this type relevant for recovery processes. In present study, role cancer cell-derived monocyte differentiation profile (polarization) was investigated. Monocyte-derived (MDM) were differentiated vitro presence TMVs obtained from...
The antioxidant and anti-inflammatory activities of acylated decarboxylated gomphrenins, as well Basella alba L. fruit extract, were investigated in relation to gomphrenin, known for its high biological potential. most abundant natural namely, 6′-O-E-caffeoyl-gomphrenin (malabarin) 6′-O-E-4-coumaroyl-gomphrenin (globosin), isolated from B. extract the studies. In addition, controlled thermal decarboxylation gomphrenin purified at 65–75 °C resulted formation prevalent products, including...
Abstract Extracellular vesicles (EVs) and neutrophil extracellular traps (NETs) are pivotal bioactive structures involved in various processes including inflammation. Herein we report the interactions between EVs NETs during murine endotoxemia studied situ directly vasculature (cremaster muscle, liver sinusoids) using intravital microscopy (IVM). We captured EV release real time by both non- polarized neutrophils but not cremaster vasculature. When comparing numbers of circulating origin...
Abstract Although blood monocytes exhibit significant cytotoxic activity against tumor cells, the function of infiltrating macrophages (TIM) is depressed in cancer patients. This study addresses question how antitumor response human monocytes, assessed by production cytokines (tumor necrosis factor α, TNF; IL-10; IL-12p40) and cytotoxicity, altered exposure to cells. Tumor cell−pre-exposed restimulated with cells showed significantly decreased TNF, IL-12, increased IL-10 (mRNA release)...
Selective IgA deficiency (SIgAD) is the most common form and variable immunodeficiency (CVID) symptomatic of predominant antibody deficiency. Despite differences in clinical picture, a similar genetic background suggested. A feature both disorders occurrence autoimmune conditions. Regulatory T cells (Tregs) are major immune cell type that maintains tolerance. As different types abnormalities Treg have been associated with primary (PID) patients, our study we aimed to analyze gene expression...
Tumour cells release membrane micro(nano)fragments called tumour-derived microvesicles (TMV) that are believed to play an important role in cancer progression. TMV suppress/modify antitumour response of the host, but there is also some evidence for their direct interaction with cells. In patients present body fluid and tumour microenvironment. The study aimed at characterization whole types/subpopulations, not only exosomes, from newly established gastric cell line (called GC1415) define...