A5090728771- Extracellular vesicles in disease
- Parasites and Host Interactions
- Parasite Biology and Host Interactions
- Helminth infection and control
- MicroRNA in disease regulation
- Viral Infections and Vectors
- Polysaccharides and Plant Cell Walls
- Parasitic infections in humans and animals
- Parasitic Infections and Diagnostics
- Insect symbiosis and bacterial influences
- Microbial Metabolites in Food Biotechnology
- Trypanosoma species research and implications
- Antifungal resistance and susceptibility
- MXene and MAX Phase Materials
- RNA and protein synthesis mechanisms
- Coccidia and coccidiosis research
- Studies on Chitinases and Chitosanases
- Mosquito-borne diseases and control
- Glycosylation and Glycoproteins Research
- Reproductive System and Pregnancy
- Fungal and yeast genetics research
- Bacterial Infections and Vaccines
- Nanoplatforms for cancer theranostics
- Circular RNAs in diseases
- Cell Adhesion Molecules Research
Universitat de València
2016-2025
Instituto de Investigación Sanitaria La Fe
2016-2025
Redondo Optics (United States)
2021
Universidad de Granada
2020
Czech Academy of Sciences, Institute of Biotechnology
2020
Jagiellonian University
2014
University Medical Center
2014
Pohang University of Science and Technology
2014
AGH University of Krakow
2014
Cambridge University Press
2011
In the past decade, extracellular vesicles (EVs) have been recognized as potent vehicles of intercellular communication, both in prokaryotes and eukaryotes. This is due to their capacity transfer proteins, lipids nucleic acids, thereby influencing various physiological pathological functions recipient parent cells. While intensive investigation has targeted role EVs different processes, for example, cancer autoimmune diseases, EV-mediated maintenance homeostasis regulation remained less...
Extracellular vesicles (EVs) are membraneous released by a variety of cells into their microenvironment. Recent studies have elucidated the role EVs in intercellular communication, pathogenesis, drug, vaccine and gene-vector delivery, as possible reservoirs biomarkers. These findings generated immense interest, along with an exponential increase molecular data pertaining to EVs. Here, we describe Vesiclepedia, manually curated compendium (lipid, RNA, protein) identified different classes...
Extracellular vesicles (EVs), such as exosomes and microvesicles, are released by different cell types participate in physiological pathophysiological processes. EVs mediate intercellular communication cell‐derived extracellular signalling organelles that transmit specific information from their of origin to target cells. As a result these properties, defined may serve novel tools for various therapeutic approaches, including (a) anti‐tumour therapy, (b) pathogen vaccination, (c)...
Abstract Motivation: Extracellular vesicles (EVs) are spherical bilayered proteolipids, harboring various bioactive molecules. Due to the complexity of vesicular nomenclatures and components, online searches for EV-related publications components currently challenging. Results: We present an improved version EVpedia, a public database EVs research. This community web portal contains identification orthologous bioinformatic tools personalized function. EVpedia includes 6879 publications, 172...
The study of host-parasite interactions has increased considerably in the last decades, with many studies focusing on identification parasite molecules (i.e. surface or excretory/secretory proteins (ESP)) as potential targets for new specific treatments and/or diagnostic tools. In parallel, few years there have been significant advances field extracellular vesicles research. Among these vesicles, exosomes endocytic origin, a characteristic size ranging from 30-100 nm, carry several atypical...
During embryo implantation, the blastocyst interacts with and regulates endometrium, endometrial fluid secreted by epithelium nurtures embryo. Here, we propose that maternal microRNAs (miRNAs) might act as transcriptomic modifier of pre-implantation Microarray profiling revealed six 27 specific, miRNAs were differentially expressed in human during window implantation – a brief phase receptivity to released into fluid. Further investigation hsa-miR-30d, expression levels which most...
Parasitic diseases affect billions of people and are considered a major public health issue. Close to 400 species estimated parasitize humans, which around 90 responsible for great clinical burden mortality rates. Unfortunately, they largely neglected as mainly endemic poor regions. Of relevance this review, there is accumulating evidence the release extracellular vesicles (EVs) in parasitic diseases, acting both parasite–parasite inter‐communication well parasite–host interactions. EVs...
Extracellular vesicles (EVs) released by parasites have important roles in establishing and maintaining infection. Analysis of the soluble vesicular secretions adult Fasciola hepatica has established a definitive characterization total secretome this zoonotic parasite. secretes at least two subpopulations EVs that differ according to size, cargo molecules site release from The larger are specialized cells line parasite gastrodermus contain zymogen 37 kDa cathepsin L peptidase performs...
In the second of a series experiments designed to identify p47nck-Src homology 3 (SH3)-binding molecules, we report cloning SAKAP II (Src A box Nck-associated protein II) from an HL60 cDNA expression library. This molecule has been identified as encoding product WASP, which is mutated in Wiskott-Aldrich syndrome patients. Studies vivo and vitro demonstrated highly specific interaction between SH3 domains p47nck protein. Furthermore, anti-Wiskott-Aldrich antibodies recognized 66 kDa by...
ABSTRACT The prevalent porcine helminth, Ascaris suum , compromises pig health and reduces farm productivity worldwide. closely related human parasite, A. lumbricoides infects more than 800 million people representing a disease burden of 1.31 disability‐adjusted life years. infections are often chronic in nature, the parasites have profound ability to modulate their hosts' immune responses. This study provides first in‐depth characterisation extracellular vesicles (EVs) from different...
ABSTRACT The utilization of extracellular vesicles (EVs) in clinical theranostics has rapidly advanced the past decade. In November 2018, International Society for Extracellular Vesicles (ISEV) held a workshop on “EVs Clinical Theranostic”. Here, we report conclusions roundtable discussions current advancement analysis technologies and provide some guidelines to researchers field consider use EVs application. main challenges requirements EV separation characterization strategies, quality...
Abstract Plasmodium vivax is the most widely distributed human malaria parasite. Previous studies have shown that circulating microparticles during P. acute attacks are indirectly associated with severity. Extracellular vesicles (EVs) therefore major components of plasma holding insights into pathological processes. Here, we demonstrate plasma-derived EVs from patients ( Pv EVs) preferentially uptaken by spleen fibroblasts h SFs) as compared to uptake healthy individuals. Moreover, this...
Over the last decade, research interest in defining how extracellular vesicles (EVs) shape cross-species communication has grown rapidly. Parasitic helminths, worm species found phyla Nematoda and Platyhelminthes, are well-recognised manipulators of host immune function physiology. Emerging evidence supports a role for helminth-derived EVs these processes highlights as an important participant cross-phylum communication. While mammalian EV field is guided by community-agreed framework...
To investigate the nature of proteins recognized by Src homology 3 (SH3) domains, a cDNA expression library was prepared from macrophages and screened with probe representing three SH3 domains p47nck. Two clones were isolated, one, designated SAKAP I (for A box Nck-associated protein I), contained carboxyl-terminal half cbl proto-oncogene product. Studies in vitro demonstrated reactivity between derived variety molecules. Wide variations this assay suggested high degree specificity inherent...
We have followed a combined proteomic approach to identify proteins of Fasciola hepatica that could be involved in host-parasite interactions. Using two-dimensional gel electrophoresis, far Western immunoblot and mass spectrometry analyses, we identified the enolase enzyme, present excretory/secretory materials F. hepatica, as human plasminogen-binding protein. This enzyme has an apparent molecular weight 47 kDa with pI ranging from 6.2 7.2. These results suggest act plasminogen receptor.