A5054297215- MXene and MAX Phase Materials
- Extracellular vesicles in disease
- Insect symbiosis and bacterial influences
- Magnesium Alloys: Properties and Applications
- Mosquito-borne diseases and control
- Evolution and Genetic Dynamics
- Vector-Borne Animal Diseases
- Trypanosoma species research and implications
- Insect behavior and control techniques
- Invertebrate Immune Response Mechanisms
- Parasitic infections in humans and animals
- Parasite Biology and Host Interactions
- Parasitic Diseases Research and Treatment
- Parasites and Host Interactions
- Insect-Plant Interactions and Control
University of Edinburgh
2018-2023
Extracellular RNA has been proposed to mediate communication between cells and organisms however relatively little is understood regarding how specific sequences are selected for export. Here, we describe a Argonaute protein (exWAGO) that secreted in extracellular vesicles (EVs) released by the gastrointestinal nematode Heligmosomoides bakeri, at multiple copies per EV. Phylogenetic gene expression analyses demonstrate exWAGO orthologues highly conserved abundantly expressed related...
Over the last decade, research interest in defining how extracellular vesicles (EVs) shape cross-species communication has grown rapidly. Parasitic helminths, worm species found phyla Nematoda and Platyhelminthes, are well-recognised manipulators of host immune function physiology. Emerging evidence supports a role for helminth-derived EVs these processes highlights as an important participant cross-phylum communication. While mammalian EV field is guided by community-agreed framework...
Argonautes are ancient proteins with well-characterised functions in cell-autonomous gene regulation and genome defense but less clear roles non-cell-autonomous processes. Extracellular have been reported across plants, animals protozoa yet their biochemical functional properties remain elusive. Here we demonstrate that an extracellular Argonaute (exWAGO) released by the rodent-infective parasitic nematode Heligmosomoides bakeri is detectable inside mouse cells during natural infection. We...
Genetic variation for resistance and disease tolerance has been described in a range of species. In Drosophila melanogaster, genetic mortality following systemic C virus (DCV) infection is driven by large-effect polymorphisms the restriction factor pastrel (pst). However, it unclear if pst contributes to tolerance. We investigated DCV challenges spanning nine orders magnitude, males females 10 Reference Panel lines carrying either susceptible (S) or resistant (R) allele. find among-line fly...
Human and animal African trypanosomiasis (HAT & AAT, respectively) remain a significant health economic issue across much of sub-Saharan Africa. Effective control AAT potential eradication HAT requires affordable, sensitive specific diagnostic tests that can be used in the field. Small RNAs blood or serum are attractive disease biomarkers due to their stability, accessibility available technologies for detection. Using RNAseq, we have identified trypanosome small RNA present at high levels...
Abstract Extracellular RNA has been proposed to mediate communication between cells and organisms however relatively little is understood regarding how specific sequences are selected for export. Here we describe a Argonaute protein (exWAGO) that secreted in extracellular vesicles (EVs) released by the gastrointestinal nematode Heligmosomoides bakeri , at multiple copies per EV. Phylogenetic gene expression analyses demonstrate exWAGO orthologues highly conserved abundantly expressed related...
Abstract Genetic variation for both resistance and disease tolerance has been described in a range of species infected with bacterial, viral fungal pathogens. In Drosophila melanogaster , genetic mortality following systemic C Virus (DCV) infection shown to be driven by large effect polymorphisms the restriction factor pastrel (pst) . However, it is unclear if pst impacts DCV titres (i.e. resistance), or also contributes tolerance. We investigated across challenge doses spanning nine orders...