A5032083133- Extracellular vesicles in disease
- RNA Research and Splicing
- Circular RNAs in diseases
- MXene and MAX Phase Materials
- Parasite Biology and Host Interactions
- MicroRNA in disease regulation
- Lipid Membrane Structure and Behavior
- Tissue Engineering and Regenerative Medicine
- Characterization and Applications of Magnetic Nanoparticles
- RNA Interference and Gene Delivery
- Parasites and Host Interactions
- Force Microscopy Techniques and Applications
- Bacterial Infections and Vaccines
- Fungal Biology and Applications
- Galectins and Cancer Biology
- Mesenchymal stem cell research
- Complement system in diseases
- Pneumocystis jirovecii pneumonia detection and treatment
- Fermentation and Sensory Analysis
- Cytomegalovirus and herpesvirus research
- Infectious Aortic and Vascular Conditions
- Microbial Natural Products and Biosynthesis
Aarhus University
2014-2025
Aarhus University Hospital
2022-2025
Aalborg University
2024
University of Oxford
2024
University of Southern Denmark
2024
Cells release a mixture of extracellular vesicles, amongst these exosomes, that differ in size, density and composition. The standard isolation method for exosomes is centrifugation fluid samples, typically at 100,000×g or above. Knowledge the effect discrete ultracentrifugation speeds on purification from different cell types, however, limited.We examined applying differential g-forces ranging 33,000×g to 200,000×g exosome yield purity, using 2 unrelated human lines, embryonic kidney HEK293...
Abstract Exosomes are small secreted vesicles that can transfer their content to recipient cells. In cancer, exosome secretion has been implicated in tumor growth and metastatic spread. this study, we explored the possibility exosomal pathways might discard tumor-suppressor miRNA restricts progression. Secreted characterized from isogenic bladder carcinoma cell lines with differing potential were uncoupled binding target transcripts or AGO2–miRISC complex. cells, observed a relative increase...
The mechanical properties of extracellular vesicles (EVs) are known to influence their biological function, in terms of, e.g., cellular adhesion, endo/exocytosis, uptake, and mechanosensing. EVs have a characteristic nanomechanical response which can be probed via force spectroscopy (FS) exploited single them out from nonvesicular contaminants or discriminate between subtypes. However, measuring the characteristics individual FS is labor-intensive time-consuming task, usually limiting this...
Abstract Extracellular vesicles (EVs) large‐scale production is a crucial point for the translation of EVs from discovery to application EV‐based products. In October 2021, International Society Vesicles (ISEV), along with support by FET‐OPEN projects, “The Vesicle Foundry” (evFOUNDRY) and “Extracellular natural source tailor‐made nanomaterials” (VES4US), organized workshop entitled “massivEVs” discuss potential challenges This report gives an overview topics discussed during “massivEVs”,...
Over the last decade, research interest in defining how extracellular vesicles (EVs) shape cross-species communication has grown rapidly. Parasitic helminths, worm species found phyla Nematoda and Platyhelminthes, are well-recognised manipulators of host immune function physiology. Emerging evidence supports a role for helminth-derived EVs these processes highlights as an important participant cross-phylum communication. While mammalian EV field is guided by community-agreed framework...
Abstract Parasitic helminths secrete extracellular vesicles (EVs) into their host tissues to modulate immune responses, but the underlying mechanisms are poorly understood. We demonstrate that Ascaris EVs efficiently internalised by monocytes in human peripheral blood mononuclear cells and increase percentage of classical monocytes. Furthermore, EV treatment induced a novel anti‐inflammatory phenotype characterised CD14 + , CD16 − CC chemokine receptor 2 (CCR2 ) programmed death‐ligand 1...
Abstract Helminths survive within their host by secreting immunomodulatory compounds, which hold therapeutic potential for inflammatory conditions. Helminth‐derived extracellular vesicles (EVs) are one such component proposed to possess activities. Due the recent discovery of helminth EVs, standardised protocols EV separation lacking. Excretory/secretory products porcine helminth, Ascaris suum , were used compare three methods: Size exclusion chromatography (SEC), ultracentrifugation (UC)...
In the last two decades, extracellular vesicles (EVs) from three domains of life, Archaea, Bacteria and Eukaryotes, have gained increasing scientific attention. As such, role EVs in host-pathogen communication immune modulation are being intensely investigated. Pivotal to EV research is determination how where taken up by recipient cells organs vivo, which requires suitable tracking strategies including labelling. Labelling often performed post-isolation increases risks non-specific...
The intestinal helminth Ascaris lumbricoides infects over 800 million people. Infections are often chronic and immunity is not sterilizing due to host-immune modulation, therefore reinfection common after antihelmintic treatment. We have previously demonstrated a role for spp. extracellular vesicles (EVs) in host immune modulation but whether EVs recognized by the adaptive system present systemically remains unknown. Therefore, we employed well-established trickle infection model pigs mimic...
Abstract We herein describe an Atomic Force Microscopy (AFM)-based experimental procedure which allows the simultaneous mechanical and morphological characterization of several hundred individual nanosized vesicles within hour timescale. When deposited on a flat rigid surface from aqueous solution, are deformed by adhesion forces into oblate spheroids whose geometry is direct consequence their stiffness. AFM image analysis can be used to quantitatively measure contact angle vesicles,...
In the last two decades, extracellular vesicles (EVs) from three domains of life, archae, bacteria and eukaryota, have gained increasing scientific attention. As such, role EVs in host-pathogen communication immune modulation are being intensely investigated. Pivotal to EV research is determination how where taken up by recipient cells organs vivo, which requires suitable tracking strategies including labelling. Labelling often performed post-isolation increases risks non-specific labelling...
The Apiospora genus comprises filamentous fungi with promising potential, though its full capabilities remain undiscovered. In this study, we present the first genome assembly of an arundinis isolate, demonstrating a highly complete and contiguous estimated to 48.8 Mb, N99 3.0 Mb. Our analysis predicted total 15,725 genes, functional annotations for 13,619 them, revealing fungus capable producing very high amounts carbohydrate-active enzymes (CAZymes) secondary metabolites. Through...
<title>Abstract</title> Background Mesenchymal stem cell (MSC) therapy holds great potential as a treatment form, yet efficacy and safety concerns with persist. The beneficial effects of MSCs are often attributed to their secretome, including extracellular vesicles (EVs). EVs carry biologically active molecules, protected by lipid bilayer. However, several barriers hinder large-scale MSC EV production. A serum-free culturing approach is preferred for producing clinical-grade MSC-derived but...
Renal fibrosis, characterized by excessive extracellular matrix accumulation, leads to a progressive decline of renal function and is common endpoint chronic kidney disease (CKD). Current treatments primarily focus on managing underlying diseases, offering limited direct intervention for the fibrotic process. This study explores anti-fibrotic potential human adipose-derived mesenchymal stromal cells (MSCs) their derived vesicles (EVs) in context CKD, emphasizing effects systemic versus local...
<p>Effect of RAB27A, RAB27B, and NSMASE2 inhibition on cellular migration invasion. Bioinformatic analysis mRNA target sites, AGO2 immunoprecipitation. Analysis genomic alterations in the miR23b locus correlation between expression methylation selected miRNAs (TCGA dataset).</p>
<p>Characterization of cells upon miRNA overexpression and knockdown.</p>
<p>Analysis of RAB27A and RAB27B expression genomic alterations in clinical samples. Effect Rab27b knockdown on cell morphology long-term growth kinetics.</p>
<p>NTA analysis, STR Pearson correlation values of miRNA expression in exosomes, and mRNA target abundance cells vs. exosomes.</p>