Tom A. P. Driedonks
A5055254489- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- RNA Research and Splicing
- RNA modifications and cancer
- Reproductive System and Pregnancy
- SARS-CoV-2 and COVID-19 Research
- Molecular Biology Techniques and Applications
- Bacterial Infections and Vaccines
- interferon and immune responses
- Cell Adhesion Molecules Research
- Viral Infections and Vectors
- Complement system in diseases
- Energy Harvesting in Wireless Networks
- RNA Interference and Gene Delivery
- Long-Term Effects of COVID-19
- Infectious Aortic and Vascular Conditions
- Sarcoma Diagnosis and Treatment
- Parvovirus B19 Infection Studies
- RNA regulation and disease
- Phagocytosis and Immune Regulation
- Immunotherapy and Immune Responses
- Infant Nutrition and Health
- Polymer Surface Interaction Studies
Utrecht University
2017-2024
Johns Hopkins University
2020-2024
Johns Hopkins Medicine
2020-2024
University Medical Center Utrecht
2022-2024
La Trobe University
2022
German Cancer Research Center
2022
Heidelberg University
2022
Hospital Universitario de La Princesa
2017
ABSTRACT The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions extracellular vesicles (EVs), collective term covering various subtypes cell‐released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, many other names. However, specific issues arise when working with these entities, whose size amount often make them difficult to obtain as relatively...
Extracellular vesicles (EVs), through their complex cargo, can reflect the state of cell origin and change functions phenotypes other cells. These features indicate strong biomarker therapeutic potential have generated broad interest, as evidenced by steady year-on-year increase in numbers scientific publications about EVs. Important advances been made EV metrology understanding applying biology. However, hurdles remain to realising EVs domains ranging from basic biology clinical...
The release of RNA-containing extracellular vesicles (EV) into the milieu has been demonstrated in a multitude different vitro cell systems and variety body fluids. EV are limelight for their capacity to communicate genetically encoded messages other cells, suitability as candidate biomarkers diseases, use therapeutic agents. Although EV-RNA attracted enormous interest from basic researchers, clinicians, industry, we currently have limited knowledge on which mechanisms drive regulate RNA...
Several vaccines have been introduced to combat the coronavirus infectious disease-2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome 2 (SARS-CoV-2). Current SARS-CoV-2 include mRNA-containing lipid nanoparticles or adenoviral vectors that encode Spike (S) protein of SARS-CoV-2, inactivated virus, subunits. Despite growing success in worldwide vaccination efforts, additional capabilities may be needed future address issues such as stability and storage requirements, need...
Abstract Extracellular vesicles (EVs) have potential in disease treatment since they can be loaded with therapeutic molecules and engineered for retention by specific tissues. However, questions remain on optimal dosing, administration pharmacokinetics. Previous studies addressed biodistribution pharmacokinetics rodents, but little evidence is available larger animals. Here, we investigated the of Expi293F‐derived EVs labelled a highly sensitive nanoluciferase reporter (palmGRET) non‐human...
Mammalian milk is not only a source of nutrition for the newborn, but also contains various components that regulate further development. For instance, an abundant microRNAs (miRNAs), which are evolutionary conserved small non-coding RNAs involved in post-transcriptional regulation target mRNA. MiRNAs present can occur extracellular vesicles (EV), nanosized membrane released by many cell types as means intercellular communication. The EV protects enclosed miRNAs from degradation and harbors...
Abstract Human stem‐cell‐derived extracellular vesicles (EVs) are currently being investigated for cell‐free therapy in regenerative medicine applications, but the lack of noninvasive imaging methods to track EV homing and uptake injured tissues has limited refinement optimization approach. Here, we developed a new labelling strategy prepare magnetic EVs (magneto‐EVs) allowing sensitive yet specific MRI tracking systemically injected therapeutic EVs. This relies on use ‘sticky’ particles,...
Abstract Extracellular vesicles (EVs) large‐scale production is a crucial point for the translation of EVs from discovery to application EV‐based products. In October 2021, International Society Vesicles (ISEV), along with support by FET‐OPEN projects, “The Vesicle Foundry” (evFOUNDRY) and “Extracellular natural source tailor‐made nanomaterials” (VES4US), organized workshop entitled “massivEVs” discuss potential challenges This report gives an overview topics discussed during “massivEVs”,...
The release and uptake of nano-sized extracellular vesicles (EV) is a highly conserved means intercellular communication. molecular composition EV, thereby their signaling function to target cells, regulated by cellular activation differentiation stimuli. EV are regarded as snapshots cells are, therefore, in the limelight biomarkers for disease. Although research on EV-associated RNA has predominantly focused microRNAs, transcriptome consists multiple classes small non-coding RNAs with...
Brain tissue-derived extracellular vesicles (bdEVs) play neurodegenerative and protective roles, including in Alzheimer's disease (AD). Extracellular (EVs) may also leave the brain to betray state of CNS periphery. Only a few studies have profiled proteome bdEVs source tissue. Additionally, focusing on bdEV cell type-specific surface markers are rare.We aimed reveal pathological mechanisms inside by profiling tissue proteomes AD patients. In addition, indicate targets for capturing molecular...
Major efforts are made to characterize the presence of microRNA (miRNA) and messenger RNA in blood plasma discover novel disease-associated biomarkers. MiRNAs associated several types macromolecular structures, including extracellular vesicles (EV), lipoprotein particles (LPP) ribonucleoprotein (RNP). RNAs these complexes recovered at variable efficiency by commonly used EV- isolation methods, which causes biases inconsistencies miRNA quantitation. Besides miRNAs, various other non-coding...
Extracellular vesicles (EV) that are released by immune cells studied intensively for their functions in regulation and scrutinized potential human immunotherapy, example against cancer. In our search signals stimulate the release of functional EV dendritic we observed LPS-activated monocyte-derived (moDC) changed morphological characteristics upon contact with non-cognate activated bystander T-cells, while non-activated T-cells had no effect. Exposure to also stimulated EV-associated...
ABSTRACT Foetal calf serum (FCS) is a common supplement of cell culture medium and known source contaminating extracellular vesicles (EV) containing RNA. Because high degree sequence similarity among homologous non‐coding RNAs mammalian species, residual FCS‐RNA in may interfere the analysis EV‐RNA released by cultured cells. Recently, doubts have been raised as to whether commonly used protocols for depletion FCS‐EV efficiently remove FCS‐RNA. Moreover, technical details are vary between...
Abstract Extracellular vesicles (EVs) can be loaded with therapeutic cargo and engineered for retention by specific body sites; therefore, they have great potential targeted delivery of biomolecules to treat diseases. However, the pharmacokinetics biodistribution EVs in large animals remain relatively unknown, especially primates. We recently reported that when cell culture‐derived are administered intravenously Macaca nemestrina (pig‐tailed macaques), differentially associate subsets...
ABSTRACT Several vaccines have been introduced to combat the coronavirus infectious disease-2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome 2 (SARS-CoV-2). Current SARS-CoV-2 include mRNA-containing lipid nanoparticles or adenoviral vectors that encode Spike (S) protein of SARS-CoV-2, inactivated virus, subunits. Despite growing success in worldwide vaccination efforts, additional capabilities may be needed future address issues such as stability and storage...
Variants of the apolipoprotein E (APOE) gene are greatest known risk factors for sporadic Alzheimer disease (AD). Three major APOE isoform alleles, ε2, ε3, and ε4, encode produce proteins that differ by only 1-2 amino acids but have different binding partner interactions. Whereas ε2 is protective against AD relative to ε4 associated with an increased development. However, role in regulation pathogenesis has remained largely undetermined. Extracellular vesicles (EVs) lipid bilayer-delimited...
Abstract Cells can communicate via the release and uptake of extracellular vesicles (EVs), which are nano‐sized membrane that transfer protein RNA cargo between cells. EVs contain microRNAs various other types non‐coding RNA, Y is among most abundant types. Studies on how RNAs their binding proteins sorted into have mainly focused comparing intracellular (cytoplasmic) levels these to in EVs. Besides overall transcriptional may regulate sorting EVs, process also be driven by local changes...
RNA viruses have been shown to express various short RNAs, some of which regulatory roles during replication, transcription, and translation viral genomes. However, RNAs generated from SARS-CoV-1 SARS-CoV-2 genomic remained largely unexplored, possibly due limitations the widely used library preparation methods for small deep sequencing corresponding data processing. By analyzing publicly available datasets, we observed that human Calu-3 cells infected by or accumulate multiple previously...
Abstract Extracellular vesicles (EVs) are intensively investigated for their therapeutic potential and application as drug delivery vehicle. A broad perception of favourable safety profiles low immunogenicity make EVs an attractive alternative to synthetic nanoparticles. We recently showed that repeated intravenous administration human cell‐derived into pig‐tailed macaques unexpectedly elicited antibody responses after three or more injections. This coincided with decreasing EV circulation...
Background: Extracellular vesicles (EVs) and non-coding RNAs (ncRNAs) are emerging contributors to Alzheimer’s disease (AD) pathophysiology. Differential abundance of ncRNAs carried by EVs may provide valuable insights into underlying mechanisms. Brain tissue-derived (bdEVs) particularly relevant, as they offer about the tissue origin. However, there is limited research on diverse ncRNA species in bdEVs AD. Objective: This study explored whether RNA composition isolated from post-mortem...