A5050041671- Sinusitis and nasal conditions
- Asthma and respiratory diseases
- Olfactory and Sensory Function Studies
- Allergic Rhinitis and Sensitization
- Head and Neck Surgical Oncology
- Nasal Surgery and Airway Studies
- Respiratory and Cough-Related Research
- Pediatric health and respiratory diseases
- Immune Response and Inflammation
- Biochemical Analysis and Sensing Techniques
- Advanced Chemical Sensor Technologies
- IL-33, ST2, and ILC Pathways
- Respiratory viral infections research
- Influenza Virus Research Studies
- Voice and Speech Disorders
- Bone Tumor Diagnosis and Treatments
- Neurobiology and Insect Physiology Research
- Long-Term Effects of COVID-19
- Ear Surgery and Otitis Media
- Neuroinflammation and Neurodegeneration Mechanisms
- Ophthalmology and Eye Disorders
- SARS-CoV-2 and COVID-19 Research
- Dysphagia Assessment and Management
- Bacterial Infections and Vaccines
- Meningioma and schwannoma management
Johns Hopkins University
2016-2025
Johns Hopkins Medicine
2016-2025
Harvard University
2024
Dana-Farber Cancer Institute
2024
Brigham and Women's Hospital
2023
Medical University of South Carolina
2022
WA Country Health Service
2021
Johns Hopkins Hospital
2008-2018
University of North Carolina at Chapel Hill
1997-2018
Durham VA Medical Center
2018
Current COVID‐19 vaccine candidates are administered by injection and designed to produce an IgG response, preventing viremia the syndrome. However, systemic respiratory vaccines generally provide limited protection against viral replication shedding within airway, as this requires a local mucosal secretory IgA response. Indeed, preclinical studies of adenovirus mRNA candidate demonstrated persistent virus in nasal swabs despite COVID‐19. This suggests that systemically vaccinated patients,...
Several vaccines have been introduced to combat the coronavirus infectious disease-2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome 2 (SARS-CoV-2). Current SARS-CoV-2 include mRNA-containing lipid nanoparticles or adenoviral vectors that encode Spike (S) protein of SARS-CoV-2, inactivated virus, subunits. Despite growing success in worldwide vaccination efforts, additional capabilities may be needed future address issues such as stability and storage requirements, need...
SARS-CoV-2 infection of the upper airway and subsequent immune response are early, critical factors in COVID-19 pathogenesis. By studying human biopsies vitro a hamster model vivo, we demonstrated transition nasal tropism from olfactory to respiratory epithelium as virus evolved. Analyzing each variant revealed that WA1 or Delta infect proportion neurons addition primary target sustentacular cells. The possessed broader cellular invasion capacity into submucosa, while Omicron displayed...
Inflammatory sinus and nasal disease is a common cause of human olfactory loss. To explore the mechanisms underlying rhinosinusitis-associated loss, we have generated transgenic mouse model inflammation, in which tumor necrosis factor alpha (TNF-alpha) expression induced temporally controlled manner specifically within epithelium (OE). Like disease, TNF-alpha leads to progressive infiltration inflammatory cells into OE. Using this model, defined specific phases pathologic process. An initial...
Objective Chronic rhinosinusitis with nasal polyps (CRSwNP) and eosinophilia is a disease of the upper respiratory tract for which few therapies are available. Because oral investigational drug dexpramipexole serendipitously decreased blood eosinophils in amyotrophic lateral sclerosis studies, we assessed its safety, eosinophil‐lowering activity, preliminary clinical efficacy patients CRSwNP eosinophilia. Methods Sixteen subjects CRSwNP, absolute eosinophil count (AEC) ≥ 0.300 × 10 9 /L,...
Influenza causes an acute infection characterized by virus replication in respiratory epithelial cells. The severity of influenza and other diseases changes over the life course during pregnancy women, suggesting that sex steroid hormones, such as estrogens, may be involved. Using primary, differentiated human nasal cell (hNEC) cultures from adult male female donors, we exposed to endogenous 17β-estradiol (E2) or select estrogen receptor modulators (SERMs) then infected with a seasonal A...
Significance After injury to the olfactory mucosa, neural progenitor cells residing in basal layer are capable of completely reconstituting neuroepithelium. The molecular events underlying this striking regenerative capacity not fully understood. We present evidence that damage mouse mucosa results a transient acute inflammatory response. Using genetic manipulations model epithelial repair we demonstrate key role inflammation driving reparative activity cells, process involving TNF-α...
Chronic rhinosinusitis with nasal polyps (CRSwNP) can be a severe and debilitating disease associated significant morbidity, loss of smell, sinus pressure asthma exacerbations. Eosinophils play role in the majority (85%) patients. Benralizumab, an afucosylated monoclonal antibody directed against IL-5 receptor, has powerful apoptotic effects on eosinophils.We sought to investigate therapeutic benefit inhibiting receptor using benralizumab treat polyps.Patients NP (defined by endoscopic grade...
Activated T cells have been implicated in chronic rhinosinusitis (CRS) and asthma physically interact with epithelial the airways. We now report that human airway display significant constitutive cell-surface expression of costimulatory ligands, B7-H1, B7-H2, B7-H3, B7-DC. Expression B7-H1 B7-DC was selectively induced by stimulation either BEAS2B or primary nasal (PNEC) interferon (IFN)-gamma (100 ng/ml). The combination IFN-gamma tumor necrosis factor-alpha ng/ml) better than alone....
Background Abnormalities in host mucosal immunity exist chronic rhinosinusitis with nasal polyps (CRSwNPs), but it is unclear whether this a cause or an effect of the eosinophilic inflammation and frequent microbial colonization that characterizes disease. Sinonasal epithelial cells (SNECs) are critical participants healthy antimicrobial innate immune defense. They also can promote Th2 various mediators, including interleukin (IL)-33, which induces T helper to produce cytokines. Methods...