A5021195109- Asthma and respiratory diseases
- Immune Cell Function and Interaction
- Eosinophilic Esophagitis
- Eosinophilic Disorders and Syndromes
- Mast cells and histamine
- T-cell and B-cell Immunology
- Allergic Rhinitis and Sensitization
- Food Allergy and Anaphylaxis Research
- IL-33, ST2, and ILC Pathways
- Urticaria and Related Conditions
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Respiratory viral infections research
- Research on Leishmaniasis Studies
- Immune Response and Inflammation
- Systemic Lupus Erythematosus Research
- CAR-T cell therapy research
- Respiratory and Cough-Related Research
- Gastrointestinal disorders and treatments
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Cytomegalovirus and herpesvirus research
- Single-cell and spatial transcriptomics
- PI3K/AKT/mTOR signaling in cancer
- Cytokine Signaling Pathways and Interactions
Knopp Biosciences (United States)
2016-2023
National Institute of Allergy and Infectious Diseases
2007-2016
National Institutes of Health
2007-2016
Universidade do Porto
2003
Medical College of Wisconsin
2003
Creighton University
2003
Howard Hughes Medical Institute
2000
Institut de Pharmacologie Moléculaire et Cellulaire
2000
The virus-specific CD8+ T cell responses of 21 HIV-infected patients were studied including a unique cohort long-term nonprogressors with low levels plasma viral RNA and strong proliferative to HIV Ags. HIV-specific by combination standard cytotoxic (CTL) assays, MHC tetramers, TCR repertoire analysis. frequencies cells specific the majority gene products measured flow cytometric detection intracellular IFN-gamma in response HIV-vaccinia recombinant-infected autologous B cells. Very high...
Abstract Intracellular cytokine staining (ICCS), employing fluorescently labeled MAbs detected by flow cytometry, has emerged as the premier technique for studying expression at single‐cell level. Advances in polychromatic cytometry have dramatically enhanced sophistication of ICCS investigations. can simultaneously measure multiple cytokines within a single cell, allowing detection complex phenotypes. Additionally, be measured with variety other analytes, including transcription factors,...
Objective Chronic rhinosinusitis with nasal polyps (CRSwNP) and eosinophilia is a disease of the upper respiratory tract for which few therapies are available. Because oral investigational drug dexpramipexole serendipitously decreased blood eosinophils in amyotrophic lateral sclerosis studies, we assessed its safety, eosinophil‐lowering activity, preliminary clinical efficacy patients CRSwNP eosinophilia. Methods Sixteen subjects CRSwNP, absolute eosinophil count (AEC) ≥ 0.300 × 10 9 /L,...
IL-12 plays a central role in both the induction and magnitude of primary Th1 response. A critical question designing vaccines for diseases requiring immunity such as Mycobacterium tuberculosis Leishmania major is requirements to sustain memory/effector cells vivo . This report examines antigen sustaining responses sufficient protective L. after vaccination with LACK protein (LP) plus rIL-12 DNA. It shows that, initial LP rIL-12, supplemental boosting either or necessary but not fully...
Using three-color flow cytometric analysis for the detection of intracellular cytokines, we have been able to determine exact combination cytokines produced by individual T lymphocytes. Because CD4+CD27- lymphocytes shown produce more IL-4 and IL-5 than CD4+CD27+ lymphocytes, cells from normal individuals (n = 4) helminth-infected patients were sorted magnetically subpopulations. Intracellular staining IL-4, IL-5, IFN-gamma subsequent mitogen stimulation 6 h revealed that although almost no...
CD4+ T cell infiltration is known to occur in tissues at sites of mast activation. The molecules produced and released by cells that account for this lymphocyte accumulation are poorly characterized. Here we report a chemoattractant cytokine, IL-16, stored preformed bone marrow-cultured human line, HMC-1, as demonstrated intracytoplasmic cytokine staining flow cytometry, lung cells, detected immunohistochemistry. In response the anaphylatoxin, C5a, or PMA treatment, IL-16 mRNA transcripts...
CD40/CD40 ligand interactions have a central role in the induction of both humoral and cellular immunity. In this study, we examined whether plasmid expressing CD40 ligand/trimer (CD40LT) could enhance immune responses vivo. BALB/c mice were injected with beta-galactosidase DNA or without CD40LT IL-12 DNA, assessed. Mice vaccinated beta-gal plus had striking increase Ag-specific production IFN-gamma, cytolytic T cell activity, IgG2a Ab. The mechanism by which enhanced these was further...
Murine NK1 natural T (NT) cells are a population of alphabeta that express NK cell receptors and an invariant TCR rearrangement. These rapidly produce large amounts IL-4 upon activation have been suggested to promote Th2 differentiation. We sought determine whether human analogue could be detected in PBMC, if so, characterize the usage, cytokine expression, surface phenotype this subset. Using flow cytometry, we demonstrated distinct V alpha24+, beta11+, CD56+ consistent with NT cells. Upon...
Abstract Protective immunity against Leishmania major generated by DNA encoding the LACK (Leishmania homologue of receptor for activated C kinase) Ag has been shown to be more durable than vaccination with protein plus IL-12. One mechanism account this may selective ability induce CD8+ IFN-γ-producing T cells. In regard, we previously reported that depletion cells in DNA-vaccinated mice abrogated protection when infectious challenge was done 2 wk postvaccination. study, extend these findings...
Intracellular cytokine detection by flow cytometry has emerged as the premier technique for studying production at single-cell level. Multiparameter permits simultaneous of two or more cytokines within a single cell, allowing direct T(H)1 versus T(H)2 determination. This capability, combined with high throughput inherent in instrumentation, gives intracellular staining an enormous advantage over existing techniques such ELISPOT, limiting dilution, and T cell cloning. The unit describes cells...
Each of the three Th2 cytokine genes, IL-4, IL-5, and IL-13, has different functions. We hypothesized that heterogeneity could yield subpopulations with expression effector Using multiple approaches, we demonstrate human cells are composed two major subpopulations: a minority IL-5(+) (IL-5(+), IL-4(+), IL-13(+)) majority IL-5(-) (IL-5(-), population. comprised only 20% all cells. Serial rounds in vitro differentiation initially yielded Th2, but required to generate expressed less CD27...