A5019980273- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Chronic Myeloid Leukemia Treatments
- Allergic Rhinitis and Sensitization
- Mast cells and histamine
- Immunodeficiency and Autoimmune Disorders
- Asthma and respiratory diseases
- Food Allergy and Anaphylaxis Research
- SARS-CoV-2 and COVID-19 Research
- Chronic Lymphocytic Leukemia Research
- Monoclonal and Polyclonal Antibodies Research
- Eosinophilic Disorders and Syndromes
- CAR-T cell therapy research
- Hematopoietic Stem Cell Transplantation
- Cancer Immunotherapy and Biomarkers
- Acute Myeloid Leukemia Research
- Click Chemistry and Applications
- Cell Adhesion Molecules Research
- Blood disorders and treatments
- Polyamine Metabolism and Applications
- COVID-19 Clinical Research Studies
- Diabetes and associated disorders
- Dermatology and Skin Diseases
- PI3K/AKT/mTOR signaling in cancer
Medical University of Vienna
2016-2025
Karl Landsteiner University of Health Sciences
2021-2025
Institute of Immunology
2007-2023
St Anna Children's Hospital
1995-2021
Universitätsklinik für Kinder und Jugendpsychiatrie
2021
Universitätszahnklinik Wien
2021
Christian Doppler Laboratory for Thermoelectricity
2008-2018
Edaptive Computing (United States)
2015
University of Veterinary Medicine Vienna
2014
Research Institute of Radiology
2013
Abstract Dendritic cells (DC) are the most potent APCs within immune system. We show here that highly purified CD14(bright) peripheral blood monocytes supplemented with granulocyte-monocyte (GM)-CSF plus IL-4 develop high efficacy (>95% of input cells) into DC. They neo-expressed CD1a, CD1b, CD1c, CD80, and CD5; they massively up-regulated CD40 (109-fold) HLA-DQ DP (125- 87-fold); significantly (>5-fold) HLA-DR, CD4, CD11b, CD11c, CD43, CD45, CD45R0, CD54, CD58, CD59. CD14,...
Abstract Tumor associated inflammation predicts response to immune checkpoint blockade in human melanoma. Current theories on regulation of center anti-tumor T cell responses. Here we show that tumor B cells are vital melanoma inflammation. Human express pro- and anti-inflammatory factors differentiate into plasmablast-like when exposed autologous secretomes vitro. This phenotype can be reconciled melanomas where also cell-recruiting chemokines CCL3, CCL4, CCL5. Depletion patients by...
Abstract B7‐H3 belongs to the B7 superfamily, a group of molecules that costimulate or down‐modulate T‐cell responses. Although it was shown could inhibit responses, several studies – most them performed in murine systems found act costimulatory manner. In this study, we have specifically addressed potential functional dualism human by assessing effect molecule under varying experimental conditions as well on different subsets. We show does not T cells. presence strong activating signals,...
CD8+ T cell immunity to SARS-CoV-2 has been implicated in COVID-19 severity and virus control. Here, we identified nonsynonymous mutations MHC-I-restricted epitopes after deep sequencing of 747 isolates. Mutant peptides exhibited diminished or abrogated MHC-I binding a cell-free vitro assay. Reduced mutant was associated with decreased proliferation, IFN-γ production cytotoxic activity cells isolated from HLA-matched patients. Single RNA ex vivo expanded, tetramer-sorted patients further...
Several studies have demonstrated that dendritic cells (DC) can be generated in vitro from CD34+ hemopoietic progenitor cells. The growth requirements for these are poorly characterized, however. In particular, undefined serum/plasma components seem to significantly contribute DC development. We report here the cytokine combination granulocyte-macrophage CSF (GM-CSF) plus TNF-alpha and stem cell factor (SCF) commonly used generation of serum/plasma-supplemented medium is, absence serum...
Abstract In an effort to identify immunoregulatory molecules on dendritic cells (DC), we generated and screened for mAbs capable of modulating the T cell stimulatory function DC. A particularly interesting mAb was DF272. It recognizes monocyte-derived DC, but not blood monocytes or lymphocytes, has profound immunomodulatory effects Treatment DC with intact IgG Fab DF272 enhanced their capacity. This effect accompanied by neither up-regulation costimulatory such as B7.1 (CD80), B7.2 (CD86),...
Abstract In an effort to characterize molecules with immunoregulatory potential, we raised mAbs human dendritic cells. We selected Ab that recognizes a molecule is induced on monocytes differentiated in vitro toward Retroviral expression cloning identified this as B7-H3, member of the B7 family described recently. contrast earlier report, which B7-H3 was consisting two Ig-like domains, our cDNA encoded type I membrane protein four and by us therefore named 4Ig-B7-H3. mRNA analysis well...
Polymorphonuclear granulocytes (PMNs) are thought to fulfill their role in host defense primarily via phagocytosis and release of cytotoxic compounds be inefficient antigen presentation stimulation specific T cells. Dendritic cells (DCs), contrast, potent antigen-presenting with the unique capacity initiate primary immune responses. We demonstrate here that highly purified lactoferrin-positive immediate precursors end-stage neutrophilic PMN (PMNp) can reverted functional maturation program...
CD27, a tumor necrosis factor receptor family member, interacts with CD70 and influences T-, B- NK-cell functions. Disturbance of this axis impairs immunity memory generation against viruses including Epstein Barr virus (EBV), influenza, others. CD27 is commonly used as marker B cells for the classification B-cell deficiencies common variable immune deficiency. Flow cytometric immunophenotyping expression analysis on lymphoid was followed by capillary sequencing in index patients, their...
Primary immunodeficiency disorders enable identification of genes with crucial roles in the human immune system. Here we study patients suffering from recurrent bacterial, viral and Cryptosporidium infections, identify a biallelic mutation MAP3K14 gene encoding NIK (NF-κB-inducing kinase). Loss kinase activity mutant NIK, predicted by silico analysis confirmed functional assays, leads to defective activation both canonical non-canonical NF-κB signalling. Patients mutated exhibit B-cell...
The transcription factor STAT1 is important in natural killer (NK) cells, which provide immediate defense against tumor and virally infected cells. We show that mutation of a single phosphorylation site (Stat1-S727A) enhances NK cell cytotoxicity range accompanied by increased expression perforin granzyme B. Stat1-S727A mice display significantly delayed disease onset cell-surveilled models including melanoma, leukemia, metastasizing breast cancer. Constitutive S727 depends on...
Advanced macrolides, such as azithromycin (AZM) or clarithromycin (CLM), are antibiotics with immunomodulatory properties. Here we have sought to evaluate their in vitro influence on the activation of CD4+ T-cells. Isolated T-cells were stimulated agonistic anti-CD3/anti-CD28 monoclonal antibodies presence 0.6 mg/L, 2.5 10 mg/L 40 AZM CLM. Cell proliferation, cytokine level supernatants and cell viability was assessed. Intracellular signaling pathways evaluated using reporter lines, FACS...
Abstract Background SARS‐CoV‐2 has triggered a pandemic that is now claiming many lives. Several studies have investigated cellular immune responses in COVID‐19‐infected patients during disease but little known regarding possible protracted impact of COVID‐19 on the adaptive and innate system convalescent patients. Methods We used multiparametric flow cytometry to analyze whole peripheral blood samples determined SARS‐CoV‐2‐specific antibody levels against S‐protein, its RBD‐subunit, viral...