A5084446426- Allergic Rhinitis and Sensitization
- Food Allergy and Anaphylaxis Research
- Asthma and respiratory diseases
- Entomological Studies and Ecology
- Contact Dermatitis and Allergies
- Insects and Parasite Interactions
- Insect and Pesticide Research
- Study of Mite Species
- Antimicrobial Peptides and Activities
- Biotin and Related Studies
- Phytochemistry and Bioactive Compounds
- Mosquito-borne diseases and control
- Bacterial biofilms and quorum sensing
- Oral microbiology and periodontitis research
- Public Health and Environmental Issues
- Phytochemistry and Bioactivity Studies
- IL-33, ST2, and ILC Pathways
- Protein Hydrolysis and Bioactive Peptides
- Vector-borne infectious diseases
- Ocular Surface and Contact Lens
- Dermatological diseases and infestations
- Agricultural safety and regulations
- Biochemical and Structural Characterization
- Public Health and Social Inequalities
- Insect Utilization and Effects
University of Cartagena
2015-2024
Universidad Surcolombiana
2020
Yonsei University
2003
Biomedical Research Council
2003
University of South Florida
1996
Summary Fifty dust samples were collected from the mattresses and bedroom floors of 25 subjects with allergic asthma in Cartagena, Colombia, order to identify house mites quantitate Der p I, Derf I Blomia tropicalis allergens. The geometric mean total mite density per gram was 418 (range, 40–2280). Twenty‐two (44%) had more than 500 four, less 100. B. Dermatopha‐goides pteronyssinus found 96% 90% samples, accounting for 40.1% 35.7% mites, respectively. Cheyletus malaccensis, Chortoglyphus...
Analysis of cross-reactivity between the nematode Ascaris ssp. and dust mites, two important allergen sources in tropics, will contribute understanding their influence on asthma atopy. The objective this study was to investigate immunoglobulin E (IgE) domestic mites tropics.Sera from 24 asthmatic patients were used ELISA immunoblotting IgE-binding inhibition assays using Ascaris, Blomia tropicalis Dermatophagoides pteronyssinus extracts recombinants Blo t 10, ABA-1 13 as competitors....
Allergic diseases triggered by mite allergens include allergic rhinoconjunctivitis, asthma, atopic dermatitis and other skin diseases. Since the early discovery of allergenic role mites genus Dermatophagoides in mid 1960s, numerous species have been described as source capable sensitizing inducing symptoms sensitized genetically predisposed individuals. The main sources house dust worldwide are fecal pellets D. pteronyssinus, farinae, Euroglyphus maynei storage Blomia tropicalis,...
To characterize the allergens of Blomia tropicalis, a cDNA library was constructed and screened with allergic sera from asthmatic patients. One clone, Bt6, subcloned sequenced. The nucleotide sequence 934 bp length shows 390-bp reading frame which encodes 130-amino acid protein MW 14.8 kD. No potential glycosylation site found in predicted protein. inferred amino has no homology to known allergens. It cyto-solic fatty acid-binding (FABP) signature at 5–22 residues, 42.3% identity Sm14-FABP...
Summary The 13q33–34 region harbours a susceptibility locus to Ascaris lumbricoides, although the underlying genes are unknown. Immunoglobulin (Ig)E and IgG confer protective immunity here we sought investigate in an endemic population whether LIG4, TNFSF13B IRS2 influence IgE levels against ABA-1 allergen as putative resistance marker. Mite-allergic asthmatic patients were analysed for potential relationships between predisposition allergy. One thousand sixty-four subjects from Cartagena,...
Summary In some tropical areas the mite Blomia tropicalis is a clinically important allergenic component of house dust, inducing specific IgE immune response in patients with allergic respiratory diseases such as asthma and rhinitis. The identification allergens this necessary to obtain appropriate reagents for diagnostic treatment procedures. We carried out study using immunoblotting detect B. . Our results demonstrate that has one major allergen (11–13kDa) three other about 50% binding...
Saliva and muscle-derived mosquito allergens have been purified characterized. However, the complete set of remains to be elucidated. In this study, we identified characterized IgE-binding proteins from species Aedes aegypti.Serum was obtained 15 allergic individuals with asthma and/or rhinitis sensitized mosquito. IgE binding determined by ELISA. Total freeze-dried bodies A. aegypti were extracted IgE-reactive 2D gel electrophoresis, followed Western blot pooled or individual sera. spots...
Summary The prevalence of specific IgE to the storage mites Aleuroglyphus ovatus (Ao) and Chortoglyphus arcuatus (Ca) was studied in 77 individuals with allergic asthma and/or chronic rhinitis. All these had a positive skin test (weal ≥ 3 mm) extracts Dermatophagoides pteronyssimis (Dp) farinac (Df) . Sera from 29 non‐atopic were used as controls. A RAST considered when serum bound ≥1% total counts added. Dp 75.3%, at least one two ( Ao Ca), 76.6%. Among patients 79.3% 75.8% Ca,...
Differences in the IgE response to isoallergens could have clinical implications; therefore, its analysis will contribute design of better strategies for diagnosis and treatment allergic respiratory diseases. Several isoforms been described from mites but there is no information about impact Blomia tropicalis isoallergens.To evaluate differences against two Blo t 12 isoallergens.The IgE-binding properties were analysed by ELISA, a skin prick test ELISA cross-inhibition. Epitope mapping was...
The mosquito Aedes aegypti is a potential source of important clinically relevant allergens. However, the allergenicity and cross-reactivity most these has not been fully described.Natural wild-type tropomyosin was purified by size exclusion anionic-exchange chromatography from an A. extract. Further characterization accomplished MALDI-TOF/TOF. Two recombinant variants were obtained expression in Escherichia coli. Specific IgE measurement ELISA skin tests for extract performed 12 patients...
<b>Background:</b> We have previously described a cDNA (clone Bt6) encoding novel allergen from Blomia tropicalis, which showed sequence similarities to the FABP/P2/cellular retinoic acid binding protein/cellular retinol protein, family of cytosolic lipid transport proteins (cLTPs). This work was planned better characterize this official name Blo t 13 had been assigned. <b>Methods:</b> Fluorescence–based ligand assays and secondary structure analysis by circular...
<b>Background:</b> In tropical climates, sensitization to Bloma tropicalis and Dermatophagoides pteronyssinus is high mainly directed species–specific allergens. There some cross–reactivity between extracts of these mites, probably due the group 5 allergens that have sequence homology. <b>Objective Methods:</b> We used radioallergosorbent test (RAST), RAST inhibition immunoblotting experiments investigate recombinant BtM Der p 5, expressed as glutathione S–transferase...
Aedes aegypti and Dermatophagoides pteronyssinus contain important allergens including cross-reactive tropomyosins. However, the functional clinical relevance of their cross-reactivity is still debated.To analyse humoral cellular recombinant Aed a 10.01, 10.02 Der p 10.Sera from 15 Austrian house dust mite-allergic, 10-sensitized individuals were tested for IgE reactivity to tropomyosins in ELISA, inhibition ELISA basophil activation tests. BALB/c mice immunized with 10.01 or 10.02, sera...
Only few allergens derived from house dust mite (HDM) species have been evaluated in terms of their potential to induce allergic inflammation. In this study, we aimed evaluate different aspects the allergenicity and allergenic activity Blo t 2, a Blomia tropicalis allergen. 2 was produced as recombinant protein Escherichia coli. Its tested humans by skin prick test basophil activation assays, mice, passive cutaneous anaphylaxis model airway Sensitization rate (54.3%) similar that found 21...
Background: Mites of the genus Suidasia are commonly found in house dust and may play an allergenic role exposed populations. However, potential clinical impact this has not been well established. The main objective project was to evaluate mite medanensis . Methods: An extract S. prepared allergen composition determined by immunoblot. Specific IgE antibody levels Blomia tropicalis were evaluated radioallergosorbent (RAST) sera 97 allergic asthmatic patients 50 nonallergic subjects....