A5084713098- Allergic Rhinitis and Sensitization
- Food Allergy and Anaphylaxis Research
- Insect and Pesticide Research
- Asthma and respiratory diseases
- Genomics and Chromatin Dynamics
- Insects and Parasite Interactions
- Hydraulic Fracturing and Reservoir Analysis
- T-cell and B-cell Immunology
- Contact Dermatitis and Allergies
- Immunotherapy and Immune Responses
- Drilling and Well Engineering
- Vector-borne infectious diseases
- RNA Research and Splicing
- Drug-Induced Hepatotoxicity and Protection
- Chromosomal and Genetic Variations
- Biofuel production and bioconversion
- Dermatology and Skin Diseases
- Water Systems and Optimization
- CRISPR and Genetic Engineering
- Hydrocarbon exploration and reservoir analysis
- Banana Cultivation and Research
- Plant Toxicity and Pharmacological Properties
- Ethnobotanical and Medicinal Plants Studies
- Insect Resistance and Genetics
- DNA Repair Mechanisms
Inmunotek (Spain)
2014-2025
Illinois College
2021-2023
University of Illinois Urbana-Champaign
2021-2023
University of Illinois Chicago
2019-2023
University of Cartagena
2010-2021
Universidad Complutense de Madrid
2014-2018
Corporación Universitaria Rafael Nuñez
2014
Ecopetrol (Colombia)
2007
Universidad Surcolombiana
2007
Abstract The mouse Igh locus is organized into a developmentally regulated topologically associated domain (TAD) that divided subTADs. Here we identify series of distal V H enhancers (E VH s) collaborate to configure the locus. E s engage in network long-range interactions interconnect subTADs and recombination center at D J gene cluster. Deletion 1 reduces rearrangement its vicinity alters discrete chromatin loops higher order conformation. Reduction 11 used anti-PtC responses likely cause...
Polysensitized patients require allergen immunotherapy (AIT) targeting multiple allergens. However, combining extracts can lead to instability and reduced efficacy particularly due the high proteolytic activity of house dust mite (HDM) While is known that glutaraldehyde cross-linking may reduce enzymatic activity, its ability stabilize multi-allergen formulations protect key allergens from degradation remains unexplored. To evaluate impact polymerization on stability immunogenicity HDM grass...
Saliva and muscle-derived mosquito allergens have been purified characterized. However, the complete set of remains to be elucidated. In this study, we identified characterized IgE-binding proteins from species Aedes aegypti.Serum was obtained 15 allergic individuals with asthma and/or rhinitis sensitized mosquito. IgE binding determined by ELISA. Total freeze-dried bodies A. aegypti were extracted IgE-reactive 2D gel electrophoresis, followed Western blot pooled or individual sera. spots...
The mosquito Aedes aegypti is a potential source of important clinically relevant allergens. However, the allergenicity and cross-reactivity most these has not been fully described.Natural wild-type tropomyosin was purified by size exclusion anionic-exchange chromatography from an A. extract. Further characterization accomplished MALDI-TOF/TOF. Two recombinant variants were obtained expression in Escherichia coli. Specific IgE measurement ELISA skin tests for extract performed 12 patients...
Aedes aegypti and Dermatophagoides pteronyssinus contain important allergens including cross-reactive tropomyosins. However, the functional clinical relevance of their cross-reactivity is still debated.To analyse humoral cellular recombinant Aed a 10.01, 10.02 Der p 10.Sera from 15 Austrian house dust mite-allergic, 10-sensitized individuals were tested for IgE reactivity to tropomyosins in ELISA, inhibition ELISA basophil activation tests. BALB/c mice immunized with 10.01 or 10.02, sera...
The house dust mite (HDM) Dermatophagoides pteronyssinus is an important risk factor for asthma and rhinitis. Allergen specific immunotherapy that based on recombinant proteins has been proposed the safer more efficient treatment of allergic diseases. aim this study was to design obtain a hybrid protein (DPx4) containing antigenic regions allergens Der p 1, 2, 7, 10 from mite. DPx4 produced in Escherichia coli its folding determined by circular dichroism. Non-denaturing dot-blot, ELISA,...
Papular urticaria (PU) is a common insect bite skin hypersensitivity in tropical countries. In order to gain insight into its causal allergens, we aimed evaluate cellular and humoral immune responses the recombinant salivary antigen Cte f 2 from cat flea Ctenocephalides felis.Sixty patients with PU 27 healthy controls were included this study. Specific IgE, IgG, IgG1, IgG4 against C. felis extract determined by ELISA. The T-cell response was analyzed using carboxyfluorescein succinimidyl...
Allergen Immunotherapy (AIT) is an effective treatment of allergic respiratory diseases induced by the inhalation house dust mite allergens.To evaluate efficacy and safety glutaraldehyde polymerized allergen extracts using a mixture Dermatophagoides pteronyssinus, D. farinae Blomia tropicalis in individuals residing Colombia.Two hundred fifty (250) patients with rhinoconjunctivitis with, or without asthma sensitized to B. were included. A glutaraldehyde-modified extract containing was...
Immunoglobulin (Ig) diversification occurs via somatic hypermutation (SHM) and class switch recombination (CSR), is initiated by activation‐induced deaminase (AID), which converts cytosine to uracil. Variable (V) region genes undergo SHM create amino acid substitutions that produce antibodies with higher affinity for antigen. The conversion of uracil in DNA promotes mutagenesis. Two distinct repair mechanisms regulate processing Ig genes. first involves base removal the glycosylase (UNG),...
Rates of allergic diseases such as asthma and rhinitis are on the rise important health problems in every country world. Allergen specific immunotherapy with natural allergenic extracts is a treatment directed to changing course these diseases, that has reported beneficial effects majority patients. However, this difficult because complex composition extracts. The standardize and, consequently, risk anaphylactic shock increased; furthermore, sensitization can occur other antigens present...
ABSTRACT The Igh locus is organized into a developmentally regulated topologically associated domain (TAD) that divided subTADs. Here we identify series of novel enhancers (NEs) collaborate to configure the locus, determine transcriptional potential in over hundred functional V H genes and their usage V(D)J recombination. NE1 engages network long-range interactions interconnect subTADs recombination center at D J gene cluster. Deletion alters discrete chromatin loops, higher order...
Justicia secunda is a plant of the family Acanthaceae that used in Centro America by traditional medicine with different therapeutic uses. In this study was evaluated median lethal concentration extracts obtained and hemolytic activity. The partial methanolic extract exhibited few toxicity (CL50 >1000 µg/mL) smaller activity (CE50> 1000 µg/mL), comparative other extracts. That suggests according to these results can be pharmacologic utilities.
The complex behavior exhibited by gas condensate reservoirs due to the existence of a two-phase system: reservoir and liquid its implications plus nature heterogeneities is subject present article which involves handling engineering concepts be interpreted, so that coupling them with pressure transient analysis using compositional simulator, we can obtain some patterns lead facilitate understanding reservoir's dynamics.Great volumes fluids are stored in Naturally Fractured Reservoirs...