A5100451733- Muscle Physiology and Disorders
- Virus-based gene therapy research
- Phagocytosis and Immune Regulation
- Immune cells in cancer
- Immune Cell Function and Interaction
- Viral Infectious Diseases and Gene Expression in Insects
- RNA Interference and Gene Delivery
- interferon and immune responses
- RNA Research and Splicing
- RNA modifications and cancer
- RNA regulation and disease
- Cancer-related molecular mechanisms research
- Viral Infections and Vectors
- HIV Research and Treatment
- Plant-derived Lignans Synthesis and Bioactivity
- Immunotherapy and Immune Responses
- Vector-borne infectious diseases
- Hepatitis C virus research
- Nuclear Structure and Function
- Genomics and Chromatin Dynamics
- Respiratory viral infections research
- Genomics and Phylogenetic Studies
- Gene expression and cancer classification
- Vector-Borne Animal Diseases
- Tissue Engineering and Regenerative Medicine
Tianjin Chengjian University
2025
Hubei University of Medicine
2023-2024
Shanghai Institute of Materia Medica
2024
First People's Hospital of Chongqing
2024
Guangzhou University of Chinese Medicine
2024
Shaoxing University
2024
BGI Group (China)
2023-2024
Guiyang Medical University
2024
Chinese Academy of Sciences
2011-2024
Changchun University of Science and Technology
2024
Hepatocellular carcinoma is one of the most common malignant tumors.M6A a novel epigenetic modification that have been emerged as vital regulators for progression HCC.However, regulatory role, clinical significance and details modification, such impact on local tumor environment, remain largely unclear.Our study showed ALKBH5 was highly expressed in HCC high expression predicted worse prognosis patients.Prediction function by tissue samples single cell sequencing Gene Set Variation...
Myostatin has been extensively documented as a negative regulator of muscle growth. inhibition is therefore considered an attractive strategy for the treatment muscle-wasting diseases such muscular dystrophies. To investigate whether systemic gene delivery myostatin propeptide (MRPO), natural inhibitor myostatin, could enhance body-wide skeletal growth, we used adeno-associated virus serotype 8 (AAV8) vectors to deliver MRPO into either normal mice or mdx mice, murine model Duchenne...
We have shown that decorin, a small leucine-rich proteoglycan, can inhibit transforming growth factor (TGF)-β1 to prevent fibrous scar formation and improve muscle healing after injury. In the decorin-treated muscle, an enhancement of regeneration is observed through histological examination. this article, we report our determination whether decorin has direct effect on myogenic cells' differentiation. Our results indicate myoblasts genetically engineered express (CD cells) differentiated...
The underpinnings of STAT3 hyperphosphorylation resulting in enhanced signaling and cancer progression are incompletely understood. Loss-of-function mutations enzymes that dephosphorylate STAT3, such as receptor protein tyrosine phosphatases, which encoded by the PTPR gene family, represent a plausible mechanism hyperactivation. We analyzed whole exome sequencing (n = 374) reverse-phase array data 212) from head neck squamous cell carcinomas (HNSCCs). most common associated with...
In this study, we explore a potential vaccine for human papillomavirus (HPV)-induced tumors, using heat shock protein as an adjuvant, peptide safety, and adeno-associated virus (AAV) gene delivery vector. The tumor was devised by constructing chimeric which contained HPV type 16 E7 cytotoxic T-lymphocyte (CTL) epitope DNA (M. C. Feltkamp, H. L. Smits, M. P. Vierboom, R. Minnaar, B. de Jongh, J. W. Drijfhout, ter Schegget, Melief, Kast, Eur. Immunol. 23:2242-2249, 1993) fused with the...
The LMNA gene encodes lamins A and C, two intermediate filament-type proteins that are important determinants of interphase nuclear architecture. Mutations in lead to a wide spectrum human diseases including autosomal dominant Emery-Dreifuss muscular dystrophy (AD-EDMD), which affects skeletal cardiac muscle. cellular mechanisms by mutations cause disease have been elusive. Here, we demonstrate defects neuromuscular junctions (NMJs) part the mechanism AD-EDMD. Two AD-EDMD mouse models show...
Abstract APOBEC3G (A3G) is a potent restriction factor of HIV-1. The N-terminal domain A3G (A3G-CD1) responsible for oligomerization and nucleic acid binding, both which are essential anti-HIV activity. As countermeasure, HIV-1 viral infectivity (Vif) binds A3G-CD1 to mediate degradation. structural basis the functions remains elusive. Here, we report crystal structures primate (rA3G-CD1) alone in complex with single-stranded DNA (ssDNA). rA3G-CD1 shares conserved core structure previously...
Mutations in the FKRP gene are associated with a wide range of muscular dystrophies from mild limb-girdle dystrophy (LGMD) 2I to severe Walker–Warburg syndrome and muscle-eye-brain disease. The characteristic biochemical feature these diseases is hypoglycosylation α-dystroglycan (α-DG). Currently there no effective treatment available. In this study, we examined adeno-associated virus serotype 9 vector (AAV9)-mediated therapy mutant mouse model proline leucine missense mutation (P448L). Our...
Invertebrate tropomyosin homologs are ligands for dectin-1.
Abstract Background Low-dose Computed Tomography (CT) is used for the detection of pulmonary nodules, but ambiguous risk evaluation causes overdiagnosis. Here, we explored significance DNA methylation 7 genes including TAC1 , CDO1 HOXA9 ZFP42 SOX17 RASSF1A and SHOX2 in blood cfDNA samples distinguishing lung cancer from benign nodules healthy individuals. Method A total 149 patients [72 mass 77 ground-glass (GGNs)], 5 48 individuals were tested analyzed this study. The lasso-logistic...
Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and characterized dysregulated immune response. Studies have shown that the SARS-CoV-2 accessory protein ORF7b induces host cell apoptosis through tumor necrosis factor alpha (TNF-α) pathway blocks production of interferon beta (IFN-β). The underlying mechanism remains to be investigated. In this study, we found facilitated viral production, inhibited RIG-I-like receptor...
Congenital muscular dystrophy (CMD) is characterized by severe muscle wasting, premature death in early childhood, and lack of effective treatment. Most the CMD cases are caused genetic mutations laminin-α2, which essential for structural integrity extracellular matrix. Here, we report that somatic gene delivery a structurally unrelated protein, miniature version agrin, functionally compensates laminin-α2 deficiency murine models CMD. Adeno-associated virus-mediated overexpression miniagrin...
Fibrosis is the response of heart and other organs to injuries. Excessive fibrosis can cause organ dysfunction or even failure. Transforming-growth factor (TGF)-beta a cytokine that induces fibroblast proliferation increases synthesis number extracellular matrix proteins including collagens. Decorin (DCN) natural antagonist TGF-beta. In current study, we investigated potential antifibrotic effects DCN gene delivery by recombinant adeno-associated viral (rAAV) vector inhibit cardiac in old,...
Abstract Duchenne muscular dystrophy (DMD) is the most common and lethal genetic muscle disease, caused by mutations in dystrophin gene. No efficacious treatment currently available. Here we report AAV vector systemic delivery therapeutic benefits of functional human minidystrophin gene a severe more reliable DMD mouse model, dystrophin/utrophin double deficiency (dys−/− : utrn−/−, dKO). These mice show many pathologic phenotypic signs typical humans including kyphosis shorter life span, all...
The catalytic activity of human cytidine deaminase APOBEC3B (A3B) has been correlated with kataegic mutational patterns within multiple cancer types. molecular basis how the N-terminal non-catalytic CD1 regulates and consequently, biological function A3B remains relatively unknown. Here, we report crystal structure a soluble A3B-CD1 variant delineate several structural elements involved in assembly, nucleic acid interactions regulation A3B. We show that (i) expressed cells exists hypoactive...
Mutations in fukutin-related protein (FKRP) gene cause a wide spectrum of disease phenotypes including the mild limb-girdle muscular dystrophy 2I (LGMD2I), severe Walker-Warburg syndrome, and muscle-eye-brain disease. FKRP deficiency results α-dystroglycan (α-DG) hypoglycosylation muscle heart, which is biochemical hallmark dystroglycanopathies. To study replacement therapy, we generated characterized new mouse model LGMD2I harboring human mutation leucine 276 to isoleucine (L276I) alleles....
APOBEC3H (A3H) is a member of the APOBEC3 subfamily DNA cytosine deaminases that are important for innate immune defense and have been implicated in cancer biogenesis. To understand structural basis A3H biochemical function, we determined high-resolution structure human performed extensive analysis. The 2.49 Å crystal reveals uniquely long C-terminal helix 6 (h6), disrupted β5 strand canonical five-stranded β-sheet core, loop 1 around Zn-active center. Mutation 7 residue, W115, RNA-mediated...
Urban overheating significantly affects thermal comfort and livability, making it essential to understand the relationship between urban form land surface temperature (LST). While horizontal dimensions of have been widely studied, vertical structures their impact on LST remain underexplored. This study investigates influence three-dimensional characteristics LST, using ECOSTRESS sensor data four machine learning models. Six morphology variables—building density (BD), mean building height...