A5006733675- SARS-CoV-2 and COVID-19 Research
- Allergic Rhinitis and Sensitization
- Immunotherapy and Immune Responses
- COVID-19 Clinical Research Studies
- Food Allergy and Anaphylaxis Research
- Asthma and respiratory diseases
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Animal Virus Infections Studies
- SARS-CoV-2 detection and testing
- Long-Term Effects of COVID-19
- Viral Infectious Diseases and Gene Expression in Insects
- Immune Cell Function and Interaction
- IL-33, ST2, and ILC Pathways
- CAR-T cell therapy research
- Contact Dermatitis and Allergies
- vaccines and immunoinformatics approaches
- Hepatitis B Virus Studies
- Dermatology and Skin Diseases
- Inflammasome and immune disorders
- Biomarkers in Disease Mechanisms
- Helicobacter pylori-related gastroenterology studies
- Celiac Disease Research and Management
- Nanowire Synthesis and Applications
- Mast cells and histamine
Medical University of Vienna
2014-2024
Christian Doppler Laboratory for Thermoelectricity
2018
Institute of Immunology
2016
Abstract Background SARS‐CoV‐2 has triggered a pandemic that is now claiming many lives. Several studies have investigated cellular immune responses in COVID‐19‐infected patients during disease but little known regarding possible protracted impact of COVID‐19 on the adaptive and innate system convalescent patients. Methods We used multiparametric flow cytometry to analyze whole peripheral blood samples determined SARS‐CoV‐2‐specific antibody levels against S‐protein, its RBD‐subunit, viral...
Abstract Background The determinants of successful humoral immune response to the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) are critical importance for design effective vaccines and evaluation degree protective immunity conferred by exposure virus. As novel variants emerge, understanding their likelihood suppression population antibody repertoires has become increasingly important. Methods In this study, we analyzed SARS‐CoV‐2 polyclonal in a large clinically...
SARS-CoV-2 has triggered a pandemic and contributes to long-lasting morbidity. Several studies have investigated immediate cellular humoral immune responses during acute infection. However, little is known about long-term effects of COVID-19 on the system.
Background Individuals with secondary immunodeficiencies belong to the most vulnerable groups succumb COVID-19 and thus are prioritized for SARS-CoV-2 vaccination. However, knowledge about persistence anamnestic responses following SARS-CoV-2-mRNA vaccinations is limited in these patients. Methods In a prospective, open-label, phase four trial we analyzed S1-specific IgG, neutralizing antibodies cytokine previously non-infected patients cancer or autoimmune disease during primary mRNA...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the ongoing global COVID-19 pandemic. One possibility to control pandemic induce sterilizing immunity through induction and maintenance of neutralizing antibodies preventing SARS-CoV-2 from entering human cells replicate in.
Patients with inflammatory bowel disease (IBD) and healthy controls received primary SARS-CoV-2-mRNA vaccination a booster after six months. Anti-TNF-α-treated patients showed significantly lower antibody (Ab) levels faster waning than α4β7-integrin-antagonist recipients controls. This prospective cohort study aimed to elucidate the underlying mechanisms on basis of circulating T-follicular helper cells (cTfh) B memory cells.We measured SARS-CoV-2- Wuhan Omicron specific Abs, B- T-cell...
Background Chimeric antigen receptor T (CART) cell therapy targeting the B specific differentiation CD19 has shown clinical efficacy in a subset of relapsed/refractory (r/r) diffuse large lymphoma (DLBCL) patients. Despite this heterogeneous response, blood pre-infusion biomarkers predicting responsiveness to CART are currently understudied. Methods Blood and serum markers, along with data DLBCL patients who were scheduled for evaluated search responsiveness. Findings Compared healthy...
In high-risk populations, allergen-specific prophylaxis could protect from sensitization and subsequent development of allergic disease. However, such treatment might itself induce allergies, thus requiring hypoallergenic vaccine formulations. We here characterized the preventive potential virus-like nanoparticles (VNP) expressing surface-exposed or shielded allergens.Full-length major mugwort pollen allergen Art v 1 was selectively targeted either to surface inner side lipid bilayer...
BackgroundImmunoglobulin(Ig)E-associated allergies result from misguided immune responses against innocuous antigens. CD4+ T lymphocytes are critical for initiating and perpetuating that process, yet the crucial factors determining whether an individual becomes sensitized towards a given allergen remain largely unknown.ObjectiveTo determine key sensitization allergy allergen.MethodsWe here created novel human cell receptor(TCR) leucocyte antigen (HLA)-DR1 (TCR-DR1) transgenic mouse model of...
Background: COVID-19, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has become a recurrent endemic disease affecting whole world. Since November 2021, Omicron and its subvariants have dominated in spread of disease. In order to prevent courses disease, vaccines are needed boost maintain antibody levels capable neutralizing Omicron. Recently, we produced characterized SARS-CoV-2 vaccine based on recombinant fusion protein consisting hepatitis B virus...
Over the years, Chinese hamster ovary (CHO) cells have emerged as major host for expressing biotherapeutic proteins. Traditional methods to generate high-producer cell lines rely on random integration(s) of gene interest but thereby left identification bottlenecks a challenging task. For comparison different producer derived from various transfections, system that provides control over transgene expression behavior is highly needed. This motivated us develop novel "DUKX-B11 F3/F" line target...
IgE-mediated allergy to birch pollen affects more than 100 million patients world-wide. Bet v 1, a 17 kDa protein is the major allergen in responsible for allergic rhinoconjunctivitis and asthma patients. Allergen-specific immunotherapy (AIT) based on therapeutic administration of 1-containing vaccines an effective treatment but no allergen-specific forms prevention are available. We developed mouse model IgE sensitization 1 subcutaneous injection aluminum-hydroxide adsorbed recombinant...
Abstract Background Antibody‐based tests are available for measuring SARS‐CoV‐2‐specific immune responses but fast T‐cell assays remain scarce. Robust T cell‐based needed to differentiate specific cellular after infection from those vaccination. Methods One hundred seventeen individuals (COVID‐19 convalescent patients: n = 40; SARS‐CoV‐2 vaccinees: 41; healthy controls: 36) were evaluated (proliferation, Th1, Th2, Th17, and inflammatory cytokines, activation‐induced marker [AIM] expression)...
CD19 is among the most relevant targets in cancer immunotherapy. However, its extracellular domain (ECD) prone to aggregation and misfolding, representing a major obstacle for development analysis of CD19-targeted therapeutics. Here, we engineered stabilized CD19-ECD (termed SuperFolder) variants, which also showed improved expression rates and, contrast wild type protein, they could be efficiently purified their monomeric forms. Despite being considerably more stable, these mutants largely...
Background Treg cells have been shown to be an important part of immune-homeostasis and IL-2 which is produced upon T cell receptor (TCR)-dependent activation lymphocytes has demonstrated critically participate in development. Objective To evaluate small molecule inhibitors (SMI) for the identification novel IL-2/Treg enhancing compounds. Materials methods We used TCR-dependent allergen-specific cytokine secretion human mouse cells, next generation messenger ribonucleic acid sequencing...
Introduction Prophylactic strategies to prevent the development of allergies by establishing tolerance remain an unmet medical need. We previously reported that transfer autologous hematopoietic stem cells (HSC) expressing major timothy grass pollen allergen, Phl p 5, on their cell surface induced allergen-specific in mice. In this study, we investigated ability allergen-expressing immune (dendritic cells, CD4 + T CD8 and CD19 B cells) induce naive mice identified as promising candidates for...
Background: COVID-19 caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a recurrent endemic disease affecting whole world. Since November 2021, Omicron and its subvariants are dominating. In order to prevent courses of disease, vaccines needed boost maintain antibody levels capable neutralizing Omicron. Recently we produced characterized SARS-CoV-2 vaccine based on recombinant fusion protein consisting hepatitis B virus (HBV)-derived PreS two wild-type...
Virus-like nanoparticles (VNP) are regarded as efficient vaccination platforms and have proven to be useful for the non-anaphylactogenic delivery of allergen-specific immunotherapy in preclinical models previously. Herein, we sought determine mode VNP uptake by antigen presenting cells (APC). Accordingly, screened a collection substances known inhibit different pathways APC. The human leukemia monocytic cell line THP-1 murine dendritic DC 2.4 were examined fluorescently labelled presence or...