A5038469920- Allergic Rhinitis and Sensitization
- Food Allergy and Anaphylaxis Research
- Asthma and respiratory diseases
- Contact Dermatitis and Allergies
- Dermatology and Skin Diseases
- Transgenic Plants and Applications
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Respiratory viral infections research
- Mast cells and histamine
- Hepatitis B Virus Studies
- vaccines and immunoinformatics approaches
- Immune Cell Function and Interaction
- Celiac Disease Research and Management
- T-cell and B-cell Immunology
- SARS-CoV-2 and COVID-19 Research
- Indoor Air Quality and Microbial Exposure
- Infant Nutrition and Health
- Tracheal and airway disorders
- Biotin and Related Studies
- Microscopic Colitis
- Viral Infections and Immunology Research
- Eosinophilic Esophagitis
- Polydiacetylene-based materials and applications
- Pediatric health and respiratory diseases
Medical University of Vienna
2016-2025
Karl Landsteiner University of Health Sciences
2021-2024
Nationwide Children's Hospital
2023
Vienna General Hospital
2010-2018
Odense University Hospital
2017
Christian Doppler Laboratory for Thermoelectricity
2007-2014
University of Vienna
2004
Grass pollen is one of the most important sources respiratory allergies worldwide.This study describes development a grass allergy vaccine based on recombinant hypoallergenic derivatives major timothy allergens Phl p 1, 2, 5, and 6 by using peptide-carrier approach.Fusion proteins consisting nonallergenic peptides from 4 PreS protein hepatitis B virus as carrier were expressed in Escherichia coli purified means chromatography. Recombinant fusion tested for allergenic activity T-cell...
We have developed a recombinant B cell epitope-based vaccine (BM32) for allergen-specific immunotherapy (AIT) of grass pollen allergy. The contains fusion proteins consisting allergen-derived peptides and the hepatitis surface protein domain preS as immunological carrier.We conducted randomized, double-blind, placebo-controlled AIT study to determine safety, clinical efficacy mechanism three subcutaneous injections BM32 doses adsorbed aluminum hydroxide versus (placebo) applied monthly...
BM32 is a grass pollen allergy vaccine based on recombinant fusion proteins consisting of nonallergenic peptides from the IgE-binding sites 4 major allergens and hepatitis B preS protein.We sought to study safety clinical efficacy immunotherapy (allergen immunotherapy) with in patients pollen-induced rhinitis controlled asthma.A double-blind, placebo-controlled, multicenter allergen field was conducted for 2 seasons. After baseline season, subjects (n = 181) were randomized received 3...
Abstract Background The determinants of successful humoral immune response to the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) are critical importance for design effective vaccines and evaluation degree protective immunity conferred by exposure virus. As novel variants emerge, understanding their likelihood suppression population antibody repertoires has become increasingly important. Methods In this study, we analyzed SARS‐CoV‐2 polyclonal in a large clinically...
Abstract Allergic inflammation is based on the cross-linking of mast cell and basophil-bound IgE Abs requires at least two binding sites for allergens, which are difficult to characterize because they often conformational in nature. We studied recognition birch pollen allergen Bet v 1, a major >100 million allergic patients. Monoclonal polyclonal raised against 1-derived peptides were used compete with patients’ 1 search sequences involved recognition. Strong inhibitions (52–75%)...
Der p 23, a new, major house dust mite (HDM) allergen that is recognized by >70% of HDM-allergic patients, has high allergenic activity and, therefore, must be considered an important component for HDM-specific immunotherapy. We constructed and characterized hypoallergenic 23 vaccine HDM Three nonallergenic peptides from the C-terminal IgE epitope-containing part (P4, P5) P6, mutant peptide containing serines instead cysteines, were identified. Peptides fused to hepatitis B virus-derived...
Abstract In the Mediterranean area, lipid transfer proteins ( LTP s) are important causes of plant‐food allergies often associated with severe allergic reactions. There, peach (Pru p 3) seems to be primary sensitizer, whereas in Central Europe, little is known about importance sensitization. this region, allergen extract‐based diagnosis complicated by co‐sensitization Bet v 1, major birch pollen allergen, its cross‐reactive food allergens, and profilins. We investigated role sensitization...
Allergens and rhinovirus infections are among the most common elicitors of respiratory diseases. We report construction a recombinant combination vaccine for allergy based on rhinovirus-derived VP1, surface protein which is critically involved in infection cells, nonallergenic peptide major grass pollen allergen Phl p 1. Recombinant hybrid molecules consisting VP1 1-derived 31 aa were expressed Escherichia coli. The did not react with IgE Abs from allergic patients lacked allergenic activity...
Human rhinoviruses (HRVs) are the major cause of common cold and account for 30–50% all acute respiratory illnesses. Although HRV infections usually harmless invade only upper tract, several studies demonstrate that is involved in exacerbation asthma. VP1 one surface-exposed proteins viral capsid important binding to corresponding receptors on human cells. Here we investigated its potential usefulness vaccination against cold. We expressed from two distantly related strains, HRV89 HRV14,...
Abstract Milk is one of the first components introduced into human diet. It also represents allergen sources, which induces IgE-mediated allergies in childhood ranging from gastrointestinal, skin, and respiratory manifestations to severe life-threatening manifestations, such as anaphylaxis. Here we isolated a cDNA coding for major cow’s milk allergen, αS1-casein, bovine mammary gland library with allergic patients’ IgE Abs. Recombinant αS1-casein was expressed Escherichia coli, purified,...
Abstract Allergen-specific immunotherapy is the only allergen-specific and disease-modifying treatment for allergy. The construction characterization of a vaccine birch pollen allergy reported. Two nonallergenic peptides, PA PB, derived from IgE-reactive areas major allergen Bet v 1 were fused to hepatitis B surface protein, PreS, in four recombinant fusion proteins containing different numbers combinations peptides. Fusion expressed Escherichia coli purified homogeneity showed lack IgE...
Fish is a frequent elicitor of severe IgE-mediated allergic reactions. Beside avoidance, there currently no allergen-specific therapy available. Hypoallergenic variants the major fish allergen, parvalbumin, for specific immunotherapy based on mutation 2 calcium-binding sites have been developed.This study sought to establish mouse model allergy resembling human disease and investigate whether rabbit IgG antibodies induced by immunization with hypoallergenic mutant carp allergen protect...
BackgroundIgE-allergen complexes induce mast cell and basophil activation thus immediate allergic inflammation. They are also important for IgE-facilitated allergen presentation to T cells by antigen-presenting cells.ObjectiveTo investigate whether the proximity of IgE binding sites on an affects immune complex shape subsequent effector in vitro vivo.MethodsWe constructed artificial allergens grafting epitopes different numbers onto a scaffold protein. The formed between corresponding was...
Abstract Background Recent data suggest that Bruton's tyrosine kinase ( BTK ) is an emerging therapeutic target in IgE receptor (Ig ER )‐cross‐linked basophils. Methods We examined the effects of four inhibitors (ibrutinib, dasatinib, AVL ‐292, and CNX ‐774) on IgE‐dependent activation histamine release blood basophils obtained from allergic patients (n=11) nonallergic donors (n=5). In addition, we these drugs growth human basophil cell line KU 812 mast HMC ‐1. Results All blockers were...
This chapter reports recent advances in the development of synthetic and genetically engineered allergen derivatives compares strategies with modifications applied to increase safety extracts for immunotherapy. It summarize experiences gained from clinical studies performed modified allergen-derivatives humans. The discusses perspectives on how use or hypoallergenic may improve specific Based epitope structural analyses biologically active recombinant molecules, versions thereof are...