A5068057349- T-cell and B-cell Immunology
- Allergic Rhinitis and Sensitization
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Food Allergy and Anaphylaxis Research
- Asthma and respiratory diseases
- Bacteriophages and microbial interactions
- Hematopoietic Stem Cell Transplantation
- Genomics and Phylogenetic Studies
- CAR-T cell therapy research
- Aortic Disease and Treatment Approaches
- Bacterial Genetics and Biotechnology
- Complement system in diseases
- Contact Dermatitis and Allergies
- Aortic aneurysm repair treatments
- Genomics and Chromatin Dynamics
- Renal Transplantation Outcomes and Treatments
- Ubiquitin and proteasome pathways
- Infectious Aortic and Vascular Conditions
- Glycosylation and Glycoproteins Research
- Cardiac and Coronary Surgery Techniques
- Transgenic Plants and Applications
- Multiple Myeloma Research and Treatments
- Immunodeficiency and Autoimmune Disorders
- Wnt/β-catenin signaling in development and cancer
Medical University of Vienna
2014-2024
Vienna General Hospital
2006-2013
NHS Blood and Transplant
2013
University of Vienna
1997-2006
Fibroblasts are the prevalent cell type and main source for extracellular matrix (ECM) in connective tissue. Depending on their origin, fibroblasts play a central role non-pathological tissue remodeling disease like fibrosis. This study examined effect of established culture conditions primary human fibroblasts, from different origins myofibroblast-like phenotype formation. We isolated aortic adventitia, lung, juvenile- adult skin investigated expression levels CD90, alpha smooth muscle...
Fish is a frequent elicitor of severe IgE-mediated allergic reactions. Beside avoidance, there currently no allergen-specific therapy available. Hypoallergenic variants the major fish allergen, parvalbumin, for specific immunotherapy based on mutation 2 calcium-binding sites have been developed.This study sought to establish mouse model allergy resembling human disease and investigate whether rabbit IgG antibodies induced by immunization with hypoallergenic mutant carp allergen protect...
A novel archaeal bacteriophage, phi Ch1, was isolated from a haloalkalophilic archaeon Natronobacterium magadii upon spontaneous lysis. The phage-cured strain N. magadii(L13) used to demonstrate infectivity of phage Ch1. turbid-plaque morphology and the fact that cells plaques were able produce indicated Ch1 is temperate phage. resembles other members Myoviridae-infecting Halobacterium species. In solution below 2M NaCl, lost its morphological stability infectivity. One- two-dimensional...
The double-stranded (ds)DNA virus phiCh1 infects the haloalkaliphilic archaeon Natrialba magadii. complete DNA sequence of 58 498 bp temperate was established, and probable functions 21 98 phiCh1-encoded open reading frames (ORFs) have been assigned. This knowledge has used to propose functional modules each required for specific during development. is terminally redundant circularly permuted therefore appears be packaged by so-called headful mechanism. presence ORFs encoding homologues...
By differential screening we isolated genes upregulated in inflammatory cytokine-stimulated human skin microvascular endothelial cells. One of these cDNAs encoded RCC1 (regulator chromosome condensation 1)-like repeats and a HECT (homologous to E6-AP C-terminus) domain, representing member the HERC (HECT domain protein) family ubiquitin ligases. The mRNA level this member, HERC5, is specifically cells by pro-inflammatory cytokines tumor necrosis factor α interleukin 1β, lipopolysaccharide...
Abstract Allergy represents a hypersensitivity disease that affects >25% of the population in industrialized countries. The underlying type I allergic immune reaction occurs predisposed atopic individuals response to otherwise harmless Ags (i.e., allergens) and is characterized by production allergen-specific IgE, an T cell response, release biologically active mediators such as histamine from mast cells basophils. Regimens permanently tolerizing still need be developed. We therefore...
Background. Mixed chimerism is an effective strategy for the induction of transplantation tolerance but toxicity recipient conditioning makes current bone marrow (BM) (BMT) protocols unsuitable widespread clinical application. Therapies promoting BM engraftment under minimal would facilitate translation this concept to clinic. Recently, we have shown that regulatory T cell (Treg) therapy has potent engraftment-enhancing effects in irradiation-free noncytotoxic BMT protocol, only if it...
The mixed chimerism approach achieves donor-specific tolerance in organ transplantation, but clinical use is inhibited by the toxicities of current bone marrow (BM) transplantation (BMT) protocols. Blocking CD40:CD154 pathway with anti-CD154 monoclonal antibodies (mAbs) exceptionally potent inducing chimerism, these mAbs are clinically not available. Defining roles donor and recipient CD40 a murine allogeneic BMT model, we show that CD4 or CD8 activation through an intact direct T cell...
Early introduction of food allergens into children's diet is considered as a strategy for the prevention allergy. The major fish allergen parvalbumin exhibits high stability against gastrointestinal digestion. We investigated whether resistance carp to digestion affects oral tolerance induction.Natural Cyp c 1, nCyp and digestion-sensitive recombinant 1 mutant, mCyp were analyzed their ability induce in murine model. Both antigens compared by gel filtration, circular dichroism measurement,...
The radial artery (RA) is a frequently used conduit in coronary bypass grafting (CABG). Endothelial injury incurred during graft harvesting promotes oxidative damage, which leads to disease and failure. We evaluated the protective effect of DuraGraft®, an endothelial damage inhibitor (EDI), on RA grafts. further compared EDI between grafts saphenous vein (SVG). Samples (n = 10) SVG 13) from 23 patients undergoing CABG were flushed preserved with either or heparinized Ringer's lactate...
Telocytes (TCs), a novel interstitial cell entity promoting tissue regeneration, have been described in various tissues. Their role inter-cellular signalling and remodelling has reported almost all human This study hypothesizes that TC also contributes to regeneration of the thoracic aorta (HTA). The understanding homeostasis regenerative potential HTA is high clinical interest as it plays crucial pathogenesis from aortic dilatation lethal dissection. Therefore, we obtained twenty-five...
Donor-specific antibodies of the IgG isotype are measured routinely for diagnostic purposes in renal transplant recipients and associated with antibody-mediated rejection long-term graft loss.
Central clonal deletion has been considered the critical factor responsible for robust state of tolerance achieved by chimerism-based experimental protocols, but split-tolerance models and clinical experience are calling this assumption into question. Although clone-size reduction through shown to be universally required achieving allotolerance, it remains undetermined whether is sufficient itself. Therapeutic Treg treatment induces chimerism in a stringent murine BM transplantation model...
The genome of the archaeal virus φCh1, infecting Natrialba magadii (formerly Natronobacterium ), is composed 58.5 kbp linear ds DNA. Virus particles contain several RNA species in sizes 100–800 nucleotides. A fraction φCh1 genomes modified within 5′‐GATC‐3′ and related sequences, as determined by various restriction enzyme digestion analyses. High performance liquid chromatography revealed a fifth base, addition to four nucleosides, which was identified N 6 ‐methyladenosine. Genetic analyses...
Therapeutic strategies for the prophylaxis of IgE-mediated allergy remain an unmet medical need. Cell therapy is emerging approach with high potential preventing and treating immunological diseases. We aimed to develop a cell-based inducing permanent allergen-specific tolerance allergy.Wild-type mice were treated allergen-expressing bone marrow cells under short course tolerogenic immunosuppression (mTOR inhibition costimulation blockade). Bone was retrieved from novel transgenic mouse...
Cell therapy with recipient Tregs achieves engraftment of allogeneic bone marrow (BM) without the need for cytoreductive conditioning (i.e., irradiation or cytotoxic drugs). Thereby mixed chimerism and transplantation tolerance are established in recipients conditioned solely costimulation blockade rapamycin. However, clinical translation would be substantially facilitated if Treg-stimulating pharmaceutical agents could used instead individualized cell therapy. Recently, it was shown that...
Abstract More than 40% of allergic patients suffer from grass pollen allergy. Phl p 1, the major timothy allergen, belongs to cross-reactive group 1 allergens that are thought initiate sensitization pollen. Repeated allergen encounter boosts allergen-specific IgE production and enhances clinical sensitivity in patients. To investigate immunological mechanisms underlying boosting secondary Ab responses epitopes involved, a murine model for was established. A B cell epitope–derived peptide...
Induction of donor-specific tolerance is still considered as the “Holy Grail” in transplantation medicine. The mixed chimerism approach virtually only that was successfully translated into clinical setting. We have previously reported successful induction and using cell therapy with recipient T regulatory cells (Tregs) to avoid cytotoxic treatment. Treg limited by availability large-scale expansion time-consuming associated risk contamination effector cells. Using a costimulation-blockade...
Up to a third of the world’s population suffers from allergies, yet effectiveness available preventative measures remains, at large, poor. Consequently, development successful prophylactic strategies for induction tolerance against allergens is crucial. In proof-of-concept studies, our laboratory has previously shown that transfer autologous hematopoietic stem cells (HSC) or B expressing major grass pollen allergen, Phl p 5, induces robust in mice. However, eventual clinical translation...
Summary Background Development of antigen‐specific preventive strategies is a challenging goal in IgE ‐mediated allergy. We have recently shown proof‐of‐concept experiments that allergy can be successfully prevented by induction durable tolerance via molecular chimerism. Transplantation syngeneic hematopoietic stem cells genetically modified to express the clinically relevant grass pollen allergen P hl p 5 into myeloablated recipients led high levels chimerism (i.e. macrochimerism) and...
Central processes in the pathogenesis of TAV- (tricuspid aortic valve) and BAV- (bicuspid associated ascending thoracic aneurysm (ATAA) development are still unknown. To gain new insights, we have collected tissue isolated smooth muscle cells aneurysmal subjected them to situ vitro analyses. We analyzed from 78 patients (31 controls, 28 TAV-ATAAs, 19 BAV-ATAAs) established 30 primary cell cultures. Analyses included histochemistry, immuno-, auto-fluorescence-based image analyses, cellular...