A5029399156- Wound Healing and Treatments
- Mesenchymal stem cell research
- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Cardiac Fibrosis and Remodeling
- Cardiac Ischemia and Reperfusion
- Hemostasis and retained surgical items
- Transplantation: Methods and Outcomes
- Body Contouring and Surgery
- Cell Adhesion Molecules Research
- Periodontal Regeneration and Treatments
- Cardiac Structural Anomalies and Repair
- Diabetic Foot Ulcer Assessment and Management
- 3D Printing in Biomedical Research
- Congenital heart defects research
- Cardiac Imaging and Diagnostics
- Extracellular vesicles in disease
- Burn Injury Management and Outcomes
- Clusterin in disease pathology
- Acute Myocardial Infarction Research
- Cardiac electrophysiology and arrhythmias
- Blood properties and coagulation
- Angiogenesis and VEGF in Cancer
- Cardiovascular Function and Risk Factors
- Immunotherapy and Immune Responses
Medical University of Vienna
2014-2023
Austrian Cluster for Tissue Regeneration
2014-2022
SBA Research
2019
Medical University of Graz
2016-2019
Wilhelminen Hospital
2019
Ludwig Boltzmann Institute for Experimental and Clinical Traumatology
2012-2017
Cedars-Sinai Medical Center
2016
In-Q-Tel
2014
Baxter (Austria)
2010-2012
Comprehensive Cancer Center Vienna
2012
Burn injuries elicit a unique and dynamic stress response which can lead to burn injury progression. Though neutrophils represent crucial players in the burn-induced immunological events, secretion pattern systemic levels of neutrophil-derived factors have not been investigated detail so far. Serum neutrophil elastase (NE), myeloperoxidase (MPO), citrullinated histone H3 (CitH3), complement factor C3a were quantified victims over 4 weeks post injury. Furthermore, potential association with...
The clinical application of doxorubicin (DOX) is severely compromised by its cardiotoxic effects, which limit the therapeutic index and cumulative dose. Liposomal encapsulation DOX (Myocet®) provides a certain protective effect against cardiotoxicity reducing myocardial drug accumulation. We aimed to evaluate transcriptomic responses anthracyclines with different profiles in translational large animal model for identifying potential alleviation strategies.We treated domestic pigs either DOX,...
The recent concept of secretome-based tissue regeneration has profoundly altered the field regenerative medicine and offers promising novel therapeutic options. In contrast to medicinal products with a single active substance, cell-derived secretomes comprise pleiotropic bioactive ingredients, representing major obstacle for reproducible drug product efficacy warranting patient safety. Good manufacturing practice (GMP)-compliant production guarantees high batch-to-batch consistency...
Fibroblasts are the prevalent cell type and main source for extracellular matrix (ECM) in connective tissue. Depending on their origin, fibroblasts play a central role non-pathological tissue remodeling disease like fibrosis. This study examined effect of established culture conditions primary human fibroblasts, from different origins myofibroblast-like phenotype formation. We isolated aortic adventitia, lung, juvenile- adult skin investigated expression levels CD90, alpha smooth muscle...
Abstract BACKGROUND. Current prognostic information in ovarian cancer is based on tumor stage, grade, and postoperative size. Reliable molecular markers are scarce. In this article, the authors describe epigenetic events a frequently deleted region chromosome 8p22 that influence expression of suppressor candidate 3 ( TUSC3 ), putative gene cancer. METHODS. Messenger RNA promoter hypermethylation were studied cell lines samples from 2 large, independent cohorts using polymerase chain...
A variety of skin substitutes that restore epidermal and dermal structures are currently available on the market. However, main focus in research clinical application lies whereas development a subcutaneous replacement (hypodermis) is often disregarded. In this study we used fibrin sealant as hydrogel scaffold to generate three-layered substitute. For hypodermal layer adipose-derived stem cells (ASCs) mature adipocytes were embedded combined with another clot fibroblasts for construction...
Secretomes from various cell sources exert strong regenerative activities on numerous organs, including the skin. Although secretomes consist of many diverse components, a growing body evidence suggests that small extracellular vesicles (EVs) account for their capacity. We previously demonstrated secretome γ-irradiated peripheral blood mononuclear cells (PBMCs) exhibits wound healing Therefore, we sought to dissect molecular composition EVs present in and compared healing-related these other...
Meaningful translational large animal models for cardiac diseases are indispensable studying disease mechanisms, development of novel therapeutic strategies, and evaluation potential drugs. For induction heart failure, hypertrophy fibrosis, a bare metal stent was implanted in the descending aorta growing pigs (n = 7), inducing pressure stress on left ventricle (group HYPI). The constant size resulted antegrade partial obstruction aortic flow with gradual increase afterload. Five sham...
Abstract Peripheral blood mononuclear cells (PBMCs) have been shown to produce and release a plethora of pro-angiogenetic factors in response γ-irradiation, partially accounting for their tissue-regenerative capacity. Here, we investigated whether certain cell subtype PBMCs is responsible this effect, the type death affects pro-angiogenic potential bioactive molecules released by γ-irradiated PBMCs. PBMC subpopulations, including CD4 + CD8 T cells, B monocytes, natural killer were isolated...
Fibrin biomatrices have been used for many years hemostasis and sealing are a well-established surgical tool. The objective of the present study was to compare two commercially available fibrin regarding effect their thrombin concentration on keratinocytes wound healing in vitro vivo. Keratinocytes showed significant differences adhesion, viability, morphology presence matrices vitro. A high (800-1,200 IU/mL) caused deteriorated cell compatibility. By using inhibitor, those could be...
We investigated the antiarrhythmic effects of ischemic preconditioning (IPC) and postconditioning (PostC) by intracardiac electrocardiogram (ECG) measured circulating microRNAs (miRs) that are related to cardiac conduction. Domestic pigs underwent 90-min. percutaneous occlusion mid left anterior coronary artery, followed reperfusion. The animals were divided into three groups: acute myocardial infarction (AMI, n = 7), preconditioning-acute (IPC-AMI) (n 9), or AMI-PostC 5). IPC was induced...
Botulinum (neuro)toxin A (BoNT) is widely used in the field of plastic and reconstructive surgery. Among treatment pain, hyperhidrosis, or aesthetic purposes, it also to enhance wound healing prevent excessive scar formation. Some clinical data already exist, but only little known on a cellular level. The aim this study was evaluate effect BoNT cells essential for vitro. Therefore, primary human keratinocytes endothelial were treated with different concentrations tested proliferation,...
Background Split-thickness skin grafting is the gold standard to cover extensive acute and chronic wounds with a well-vascularized wound bed. Although some headway has been made in developing biological agents speed up healing, there still no treatment that sufficiently replaces grafts date. The use of secretory factors adipose tissue may be feasible approach topical applications for faster healing. Methods In this study, effect conditioned media (CMs) human adipose-derived stem cells...
Diabetes and its sequelae such as diabetic foot ulcer are rising health hazards not only in western countries but all over the world. Effective, yet safe treatments desperately sought for by physicians, healthcare providers, of course patients.APOSEC, a novel, innovative drug, is tested phase I/II study MARSYAS II, where efficacy to promote healing ulcers will be determined. To this end, cell-free secretome peripheral blood mononuclear cells (APOSEC) blended with hydrogel applied topically...
An irreversible loss of subcutaneous adipose tissue in patients after tumor removal or deep dermal burns makes soft engineering one the most important challenges biomedical research. The ideal scaffold for has yet not been identified though biodegradable polymers gained an increasing interest during last years. In present study we synthesized two novel polymers, poly(ε-caprolactone-co-urethane-co-urea) (PEUU) and poly[(L-lactide-co-ε-caprolactone)-co-(L-lysine ethyl ester...
BackgroundSince numerous pathological conditions are evoked by unwanted dendritic cell (DC) activity, therapeutic agents modulating DC functions of great medical interest. In regenerative medicine, cellular secretomes have gained increasing attention and valuable immunomodulatory properties been attributed to the secretome γ-irradiated peripheral blood mononuclear cells (PBMCs). Potential effects PBMC (PBMCsec) on key not elucidated so far.MethodsWe used a hapten-mediated murine model...
Acute myocardial infarction (AMI) is a result of cardiac non-perfusion and leads to cardiomyocyte necrosis, inflammation, compromised performance. Here, we showed that the secretome γ-irradiated peripheral blood mononuclear cells (PBMCsec) improved heart function in porcine AMI model displayed beneficial long- short-term effects. As an known strongly affect gene regulation ischemia non-affected muscle distal organs, employed transcriptomics approach further study immediate molecular events...
Abstract Over the last century many studies have been performed to assess impact of fibrin sealant (FS) components on cells. Because noncovalent bonding thrombin during clot formation, we wanted further evaluate bound cell viability. Initially, quantified activity in three different, commercially available FS. This information was used prepare clots covering a range concentrations from 4 820 IU mL −1 , but which were identical with respect all other constituents. Although these did not...