A5084504971- SARS-CoV-2 and COVID-19 Research
- Immunotherapy and Immune Responses
- Rheumatoid Arthritis Research and Therapies
- Heat shock proteins research
- Systemic Lupus Erythematosus Research
- Extracellular vesicles in disease
- Peripheral Neuropathies and Disorders
- Animal Virus Infections Studies
- RNA Interference and Gene Delivery
- Glaucoma and retinal disorders
- Mesenchymal stem cell research
- Immune Response and Inflammation
- Wound Healing and Treatments
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Cell Adhesion Molecules Research
- IL-33, ST2, and ILC Pathways
- Immune cells in cancer
- Retinal and Macular Surgery
- Immunodeficiency and Autoimmune Disorders
- Heart Failure Treatment and Management
- COVID-19 Clinical Research Studies
- Intraocular Surgery and Lenses
- Monoclonal and Polyclonal Antibodies Research
- Tissue Engineering and Regenerative Medicine
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
Medical University of Vienna
2016-2025
Center for Rheumatology
2022-2023
Christian Doppler Laboratory for Thermoelectricity
2015-2021
Universitäts-Augenklinik Bonn
1999
University of Tübingen
1995
Augenklinik Heidelberg
1995
Abstract We previously showed that, when peripheral blood mononuclear cells (PBMCs) were stressed with ionizing radiation, they released paracrine factors that regenerative capacity in vitro and vivo . This study aimed to characterize the secretome of PBMCs investigate its biologically active components Bioinformatics analysis revealed irradiated differentially expressed genes encoded secreted proteins. These primarily involved (a) pro-angiogenic pathways (b) generation oxidized...
SARS-CoV-2-induced COVID-19 has led to exponentially rising mortality, particularly in immunosuppressed patients, who inadequately respond conventional vaccination.In this blinded randomised clinical trial, we compare the efficacy and safety of an additional booster vaccination with a vector versus mRNA vaccine non-seroconverted patients. We assigned 60 patients under rituximab treatment, did not seroconvert after their primary either BNT162b2 (Pfizer-BioNTech) or mRNA-1273 (Moderna),...
Structural reorganisation of the synovium with expansion fibroblast-like synoviocytes (FLS) and influx immune cells is a hallmark rheumatoid arthritis (RA). Activated FLS are increasingly recognised as critical component driving synovial tissue remodelling by interacting resulting in distinct pathotypes RA.
Objectives Bone destruction in rheumatoid arthritis is mediated by osteoclasts (OC), which are derived from precursor cells of the myeloid lineage. The role two monocyte subsets, classical monocytes (expressing CD115, Ly6C and CCR2) non-classical (which CD115 positive, but low CCR2), serving as precursors for OC still elusive. Methods We investigated CCR2 −/− mice, lack circulating monocytes, crossed into hTNFtg mice extent joint damage. analysed subsets K/BxN serum transfer flow cytometry....
Patients under rituximab therapy are at high risk for a severe COVID-19 disease course. Humoral immune responses to SARS-CoV-2 vaccination vastly diminished in B-cell-depleted patients, even after third vaccine dose. However, it remains unclear whether these patients benefit from fourth and continued affects antibody development.In this open-label extension trial, 37 rituximab-treated who received dose with either vector or mRNA-based were vaccinated time an (mRNA-1273 BNT162b2). Key...
Impaired response to COVID-19 vaccination is of particular concern in immunosuppressed patients. To determine the best strategy for this vulnerable group we performed a single center, 1:1 randomized blinded clinical trial. Patients who failed seroconvert upon two mRNA vaccinations (BNT162b2 or mRNA-1273) are receive either third dose same vector vaccine ChAdOx1 nCoV-19. Primary endpoint difference SARS-CoV-2 spike antibody seroconversion rate between and vaccinated patients four weeks after...
Objectives A third COVID-19 vaccination is recommended for immunosuppressed patients. However, data on immunogenicity and safety of a in patients with immune-mediated inflammatory diseases (IMIDs) are sparse therefore addressed within this clinical trial. Methods 60 48 healthy controls (HCs) received an mRNA vaccine. The primary endpoint was defined as the presence antibody levels against receptor-binding domain (RBD)>1500 BAU/mL IMIDs versus HCs. Further endpoints included differences...
Rheumatoid arthritis (RA) is characterized by immune dysregulation, including alterations in peripheral blood mononuclear cell (PBMC) populations and aberrant cytokine signaling. Methotrexate (MTX) the preferred first-line treatment for RA, yet its precise mechanisms of action remain incompletely understood. This study employed a multi-omics strategy-combining single-cell RNA sequencing (scRNA-seq) immunophenotyping-to identify key effector cells their cellular responses RA patients over 12...
Secretomes from various cell sources exert strong regenerative activities on numerous organs, including the skin. Although secretomes consist of many diverse components, a growing body evidence suggests that small extracellular vesicles (EVs) account for their capacity. We previously demonstrated secretome γ-irradiated peripheral blood mononuclear cells (PBMCs) exhibits wound healing Therefore, we sought to dissect molecular composition EVs present in and compared healing-related these other...
Abstract Peripheral blood mononuclear cells (PBMCs) have been shown to produce and release a plethora of pro-angiogenetic factors in response γ-irradiation, partially accounting for their tissue-regenerative capacity. Here, we investigated whether certain cell subtype PBMCs is responsible this effect, the type death affects pro-angiogenic potential bioactive molecules released by γ-irradiated PBMCs. PBMC subpopulations, including CD4 + CD8 T cells, B monocytes, natural killer were isolated...
To assess the humoral response to messenger RNA (mRNA) vaccine of patients with systemic autoimmune rheumatic disease (SARD) and effect immunosuppressive medication in a matched cohort study.Patients SARD were enrolled 1:1 for sex age healthy control (HC) subjects. Differences two doses an mRNA terms seroconversion rate (SCR) SARS-CoV-2 antibody level between groups impact treatment within assessed.We 82 HC. SCR after first dose was lower among patient group than that HC (65% compared 100%...
Abstract Background Several activities are attributed to antimicrobial peptides ( AMP s), including bacterial killing, leucocyte recruitment and angiogenesis. Despite promises of advanced cellular therapies for treatment diabetic foot ulcer, it is currently accepted that paracrine factors rather than components causative the observed effects. Whether s present in mononuclear cell MNC ) secretome ‐sec) white blood cells beneficial experimental wound healing not known. Materials methods...
Developing effective therapies against chronic wound healing deficiencies is a global priority. Thus we evaluated the safety of two different doses topically administered autologous APOSEC, secretome apoptotic peripheral blood mononuclear cells (PBMCs), in healthy male volunteers with artificial dermal wounds. Ten men were enrolled single-center, randomized, double-blinded, placebo-controlled phase 1 trial. Two wounds at upper arm generated using 4-mm punch biopsy. Each participant was...
To assess the kinetics of humoral response after first and second dose messenger RNA (mRNA) vaccines in patients with inflammatory joint diseases compared healthy controls (HC). analyse factors influencing quantity immune response.We enrolled rheumatoid arthritis (RA) seronegative spondyloarthritis (SpA), excluding those receiving B-cell depleting therapies assessed to mRNA vaccine terms seroconversion rate titre. We results a HC group analysed influence as well other characteristics on...
Abstract Introduction Women with systemic lupus erythematosus (SLE) have a higher risk for fetal and maternal complications. We aimed to investigate complications in pregnant women SLE compared high‐risk pregnancy cohort (HR) from tertiary university center standard‐risk general population (SR) the Austrian Birth Registry. Material Methods In this retrospective data analysis, we incidence of fetal/neonatal pregnancies deliveries age, body mass index delivery date—matched same department,...
The use of left ventricular assist device (LVAD) has increased considerably over the past decade; however, there is limited literature to in patient selection and monitoring. frequency adverse events remains high. We examined early expression circulating soluble ST2 (sST2), a biomarker with immunosuppressive profibrotic activity, assessed risk death at 1 year patients receiving LVAD implant.We prospectively enrolled 20 heart failure measured sST2, IL-33, IL-6 serum concentrations three weeks...
// Matthias Zimmermann 1 , Lucian Beer 1,2 Robert Ullrich 3 Dominika Lukovic 4 Elisabeth Simader Denise Traxler 1,4 Tanja Wagner 5 Lucas Nemec Lukas Altenburger Andreas Zuckermann 6 Mariann Gyöngyösi Hendrik Jan Ankersmit 1,5 and Michael Mildner 7 Christian Doppler Laboratory for Cardiac Thoracic Diagnosis Regeneration, Medical University of Vienna, Austria 2 Department Biomedical Imaging Image-Guided Therapy, Pathology, Cardiology, Surgery, Dermatology, Research Division Biology...
Abstract Trauma represents a major cause of morbidity and mortality worldwide. The endogenous inflammatory response to trauma remains not fully elucidated. Pro-inflammation in the early phase is followed by immunosuppression leading infections, multi-organ failure mortality. Heat-shock proteins (HSPs) act as intracellular chaperons but exert also extracellular functions. However, their role acute unknown. aim this study was evaluate serum concentrations HSP 27 70 severely injured patients....
ABSTRACT Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2)-induced coronavirus disease 2019 (COVID-19) has led to exponentially rising mortality, particularly in immunosuppressed patients, who inadequately respond conventional COVID-19 vaccination. In this blinded randomized clinical trial (EudraCT 2021-002348-57) we compare the efficacy and safety of an additional booster vaccination with a vector versus mRNA vaccine non-seroconverted patients. We assigned 60 patients under...
Interstitial lung disease (ILD) is a common manifestation of idiopathic inflammatory myopathies (IIM) and substantial contributor to hospitalisation, increased morbidity, mortality. In-vivo evidence ongoing tissue remodelling in IIM-ILD scarce. We aimed evaluate fibroblast activation lungs IIM-patients control individuals using ⁶⁸Ga-labelled inhibitor Fibroblast-Activation-Protein (FAPi) based positronic emission tomography computed imaging (PET/CT).
Background The question addressed is: how does the postoperative visual acuity in eyes treated with segmental buckling compare over time paired fellow eyes? Methods 107 detachments were followed prospectively. divided into: group I, macula attached (46); II, partially detached (10); III, completely (51). Mean preoperative was 20/30 20/100 and 20/400 III. operation consisted of without drainage. No eye had cerclage or vitrectomy. Results retina remained 99 during 15-year follow-up. mean all...
Objectives This study aimed to assess the duration of humoral responses after two doses SARS-CoV-2 mRNA vaccines in patients with inflammatory joint diseases and IBD booster vaccination compared healthy controls. It also analyze factors influencing quantity quality immune response. Methods We enrolled 41 rheumatoid arthritis (RA), 35 seronegative spondyloarthritis (SpA), suffering from bowel disease (IBD), excluding those receiving B-cell-depleting therapies. assessed total anti-SARS-CoV-2...