A5083173373- Allergic Rhinitis and Sensitization
- Food Allergy and Anaphylaxis Research
- Immunotherapy and Immune Responses
- Asthma and respiratory diseases
- Dermatology and Skin Diseases
- vaccines and immunoinformatics approaches
- Transgenic Plants and Applications
- RNA Interference and Gene Delivery
- Immune Response and Inflammation
- Contact Dermatitis and Allergies
- Mosquito-borne diseases and control
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Antimicrobial Peptides and Activities
- Vector-borne infectious diseases
- SARS-CoV-2 and COVID-19 Research
- Virus-based gene therapy research
- Monoclonal and Polyclonal Antibodies Research
- Toxin Mechanisms and Immunotoxins
- Advancements in Transdermal Drug Delivery
- Pharmaceutical studies and practices
- Malaria Research and Control
- Glycosylation and Glycoproteins Research
- Herpesvirus Infections and Treatments
- Vector-Borne Animal Diseases
University of Salzburg
2013-2022
Vienna Biocenter
2022
Christian Doppler Klinik
2006-2016
Tropomyosins represent clinically relevant seafood allergens but the role of mite tropomyosin, Der p 10, in house dust (HDM) allergy has not been studied detail.To express and purify a recombinant 10 with equivalent IgE reactivity as natural to evaluate its allergenic activity HDM-allergic patients.rDer was expressed Escherichia coli, purified characterized by mass spectrometry circular dichroism. It tested for 1322 patients. Detailed IgE-reactivity profiles six HDM (Der 1, 2, 5, 7, 21) were...
Driven by constantly increasing knowledge about skin immunology, vaccine delivery via the cutaneous route has recently gained renewed interest. Considering its richness in immunocompetent cells, targeting antigens to is considered be more effective than intramuscular or subcutaneous injections. However, circumvention of superficial layer skin, stratum corneum, represents major challenge for immunization. An optimal method and reliable, but also highly adaptable specific demands, should avoid...
BackgroundThe search for intrinsic factors, which account a protein's capability to act as an allergen, is ongoing. Fold stability has been identified molecular feature that affects processing and presentation, thereby influencing antigen's immunologic properties.ObjectiveWe assessed how changes in fold modulate the immunogenicity sensitization capacity of major birch pollen allergen Bet v 1.MethodsBy exploiting exhaustive virtual mutation screening, we generated mutants prototype 1 with...
Over 100 million people worldwide suffer from birch pollen allergy. Bet v 1 has been identified as the major allergen. However, molecular mechanisms of allergic sensitization, including roles and other components extract, remain incompletely understood. Here, we examined how known pollen-derived molecules influence endolysosomal processing 1, thereby shaping its allergenicity.We analyzed biochemical immunological interaction ligands with 1. We then investigated proteolytic by endosomal...
Allergens and rhinovirus infections are among the most common elicitors of respiratory diseases. We report construction a recombinant combination vaccine for allergy based on rhinovirus-derived VP1, surface protein which is critically involved in infection cells, nonallergenic peptide major grass pollen allergen Phl p 1. Recombinant hybrid molecules consisting VP1 1-derived 31 aa were expressed Escherichia coli. The did not react with IgE Abs from allergic patients lacked allergenic activity...
Abstract Milk is one of the first components introduced into human diet. It also represents allergen sources, which induces IgE-mediated allergies in childhood ranging from gastrointestinal, skin, and respiratory manifestations to severe life-threatening manifestations, such as anaphylaxis. Here we isolated a cDNA coding for major cow’s milk allergen, αS1-casein, bovine mammary gland library with allergic patients’ IgE Abs. Recombinant αS1-casein was expressed Escherichia coli, purified,...
Two main shortcomings of classical allergen-specific immunotherapy are long treatment duration and low patient compliance. Utilizing the unique immunological features skin by transcutaneous application antigen opens new approaches not only for painless vaccine delivery, but also immunotherapy. Under certain conditions, however, barrier disruption favors T helper 2-biased immune responses, which may lead to sensitizations.In a prophylactic approach, an infra-red laser device was employed,...
Cow's milk is one of the most common causes food allergy affecting approximately 2.5% infants in first years their life. However, only limited information regarding allergenic activity individual cow's allergens available.To analyse frequency IgE reactivity and to determine allergens.A nitrocellulose-based microarray, based on purified natural recombinant was used profiles using sera from 78 milk-sensitized individuals varying ages. The tested patients' for loading rat basophil leukaemia...
Abstract Gene gun immunization, i.e., bombardment of skin with DNA-coated particles, is an efficient method for the administration DNA vaccines. Direct transfection APC or cross-presentation exogenous Ag acquired from transfected nonimmune cells enables MHC-I-restricted activation CD8+ T cells. Additionally, MHC-II-restricted presentation activates CD4+ Th Being principal in epidermis, Langerhans (LC) seem ideal candidates to accomplish these functions. However, dependence on LC gene...
Abstract Wheat is an essential element in our nutrition but one of the most important food allergen sources. allergic patients often suffer from severe gastrointestinal and systemic reactions after wheat ingestion. In this study, we report molecular immunological characterization a new major allergen, Tri 36. The cDNA coding for C-terminal fragment 36 was isolated by screening seed expression library with serum IgE food-allergic patients. 369-aa protein hydrophobic 25-aa N-terminal leader...
Wheat (Triticum aestivum) is an important allergen source responsible for various clinical manifestations of allergy (i.e. food allergy, pollen respiratory to flour-Baker's asthma).The objective this study was the molecular and immunological characterization new recombinant wheat allergens evaluate their usefulness diagnosis wheat.A T. aestivum cDNA library constructed screened with serum IgE from patients suffering identify cDNAs coding allergens. The allergen-encoding were expressed in...
The incidence of allergic disorders and asthma continuously increased over the past decades, consuming a considerable proportion health care budget. Allergen-specific subcutaneous immunotherapy represents only intervention treating underlying causes type I allergies, but still suffers from unwanted side effects low compliance. There is an urgent need for novel approaches improving safety efficacy this therapy. In present study we investigated carbohydrate-mediated targeting allergens to...
Several hydrolyzed cow's milk (CM) formulas are available for avoidance of allergic reactions in CM-allergic children and prevention allergy development high-risk infants. Our aim was to compare CM regarding the presence immunoreactive components, IgE reactivity, allergenic activity, ability induce T-cell proliferation, cytokine secretion.A blinded analysis eight formulas, one nonhydrolyzed, two partially (PH), four extensively (EH), amino acid formula, using biochemical techniques specific...
Allergies and their characteristic TH2-polarized inflammatory reactions affect a substantial part of the population. Since there is increasing evidence that immune system modulates plasticity function central nervous (CNS), we investigated effects allergic lung inflammation on hippocampus – region cellular in adult brain. The focus present study was microglia, resident cells CNS, hippocampal neurogenesis, i.e. generation new neurons. C57BL/6 mice were sensitized with clinically relevant...