A5012301662- Allergic Rhinitis and Sensitization
- Food Allergy and Anaphylaxis Research
- Contact Dermatitis and Allergies
- Asthma and respiratory diseases
- Dermatology and Skin Diseases
- Transgenic Plants and Applications
- Monoclonal and Polyclonal Antibodies Research
- Plant Reproductive Biology
- Exercise and Physiological Responses
- Sports Performance and Training
- Sports injuries and prevention
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Cardiovascular Effects of Exercise
- Advanced Biosensing Techniques and Applications
- Inhalation and Respiratory Drug Delivery
- Advancements in Transdermal Drug Delivery
- Cellular transport and secretion
- Insect and Arachnid Ecology and Behavior
- Toxin Mechanisms and Immunotoxins
- Escherichia coli research studies
- Mast cells and histamine
- Protein purification and stability
- T-cell and B-cell Immunology
- RNA regulation and disease
University of Salzburg
2015-2025
Christian Doppler Klinik
2014
Abstract Since the discovery of immunoglobulin E (IgE) as a mediator allergic diseases in 1967, our knowledge about immunological mechanisms IgE‐mediated allergies has remarkably increased. In addition to understanding immune response and clinical symptoms, allergy diagnosis management depend strongly on precise identification elicitors reaction. past four decades, innovations bioscience technology have facilitated production well‐defined, highly pure molecules for component‐resolved (CRD),...
Common ragweed has been spreading as a neophyte in Europe. Elevated CO2 levels, hallmark of global climate change, have shown to increase pollen production, but their effects on allergenicity remain be elucidated.Ragweed was grown climate-controlled chambers under normal (380 ppm, control) or elevated (700 based RCP4.5 scenario) levels. Aqueous extracts (RWE) from control- -pollen were administered vivo mouse model for allergic disease (daily 3-11 days, n = 5) and employed human vitro...
Over 100 million people worldwide suffer from birch pollen allergy. Bet v 1 has been identified as the major allergen. However, molecular mechanisms of allergic sensitization, including roles and other components extract, remain incompletely understood. Here, we examined how known pollen-derived molecules influence endolysosomal processing 1, thereby shaping its allergenicity.We analyzed biochemical immunological interaction ligands with 1. We then investigated proteolytic by endosomal...
Studies of human IgE and its targeted epitopes on allergens have been very limited. We an established method to immortalize encoding B cells from allergic individuals. To develop unbiased comprehensive panel peanut-specific mAbs characterize key immunodominant antigenic regions peanut map the molecular interactions responsible for inducing anaphylaxis. Using hybridoma technology peripheral blood subjects with severe allergy, we generated a naturally occurring in manner. Isolated were...
Summary Background Birch pollen allergy represents the main cause of winter and spring pollinosis in temperate climate zone northern hemisphere sensitization towards Bet v 1, major birch allergen, affects over 100 million allergic patients. The allergen 1 has been described as promiscuous acceptor for a wide variety hydrophobic ligands. Objective In search intrinsic properties which account responsible high allergenic potential protein, we thought to investigate effects ligand‐binding on...
Ragweed pollen represents a major allergy risk factor. extracts contain five different isoforms of the allergen Amb 1. However, immunological characteristics 1 are not fully investigated. Here, we compared physicochemical and properties three most important isoforms.After purification, were physicochemically characterized, tested for antibody binding induction human T-cell proliferative responses. Their further evaluated in vitro vivo mouse model.Amb exhibited distinct patterns IgE...
Evidence has accumulated that birch pollen immunotherapy reduces rhinoconjunctivitis to of homologous trees. Therapeutic efficacy been associated with IgE-blocking IgG antibodies. We have recently shown sera collected after 16 weeks sublingual recombinant Bet v 1 (rBet 1-SLIT) display strong bioactivity for 1. Here, we assessed whether rBet 1-SLIT-induced antibodies cross-blocking activity related allergens in Fagales pollen.IgE, IgG1 and IgG4 reactivity 1, Aln g Car b Ost c Cor a Fag s Cas...
Impact of SiO 2 NP-allergen interaction on dendritic cell function altering the immune response, eventually resulting in a harmless, beneficial outcome terms allergic reactivity.
Performing multiple high-intensity interval training (HIIT) sessions in a compressed period of time (approximately 7-14 days) is called HIIT shock microcycle (SM) and promises rapid increase endurance performance. However, the efficacy HIIT-SM, as well knowledge about optimal volumes during SM endurance-trained population have not been adequately investigated. This study aims to examine effects two different types HIIT-SM (with or without additional low-intensity (LIT)) compared control...
The clinical benefit of allergen-specific immunotherapy (AIT) involves induction blocking antibodies. It is not clear if these antibodies function via steric hindrance alone or a combination levels, avidities, and epitope specificities, outcome cannot be predicted. We aim to in-depth characterize serum antibody profiles during birch pollen AIT, investigate therapy-induced for their capacity block IgE binding Bet v 1 correlate data with outcomes.Immune responses five allergic patients were...
Transforming growth factor β1 (TGFβ1) is a cytokine that exerts immunosuppressive functions, as reflected by its ability to induce regulatory T (Treg) cell differentiation and inhibit Th1 Th2 responses. Hence, peptides mimic the active core domain of TGFβ1 may be promising candidates for modulation allergic response. This study aimed investigate synthetic mimetic peptide (TGFβ1-mim) modulate immune response during sensitization grass pollen allergens.
In birch-dominated areas, allergies to pollen from trees of the order Fagales are considered be initiated by major birch allergen Bet v 1. However, sensitizing activity 1-homologs in might underestimated. Allergen-specific T-cells crucial sensitization process. The T-cell response allergens alder, hazel, oak, hornbeam, chestnut, beech, and chestnut has not yet been analyzed. Here, we characterized cellular cross-reactivity with
Allergen-specific immunotherapy (AIT) represents a curative approach for treating allergies. In the tropical and subtropical regions of world, Blomia tropicalis (Blo t 5 Blo 21) is likely dominant source indoor allergens.To generate hypoallergenic 5/Blo 21 hybrid molecule that can treat allergies caused by B tropicalis.Using in silico design proteins, we chose two proteins heterologous expression. Wild-type version, termed BTH1 BTH2, respectively, were purified ion exchange size exclusion...
AHR Airway hyperresponsiveness AIT Allergen-specific immunotherapy Alum Aluminum hydroxide APC Antigen-presenting cell AR Allergic rhinitis BALF Bronchoalveolar lavage fluids BM Bone marrow-derived BP Birch pollen BPE extract CCL20 Chemokine (C-C motif) ligand 20 DAMP Damage-associated molecular patterns DC Dendritic cells β-glucan β-(1,3)-