A5100723164- Cancer Immunotherapy and Biomarkers
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Monoclonal and Polyclonal Antibodies Research
- Single-cell and spatial transcriptomics
- Invertebrate Immune Response Mechanisms
- RNA modifications and cancer
- Acute Myeloid Leukemia Research
- RNA Interference and Gene Delivery
- Molecular Biology Techniques and Applications
- Iron Metabolism and Disorders
- Platelet Disorders and Treatments
- Cancer-related molecular mechanisms research
- Cancer Genomics and Diagnostics
- Blood Coagulation and Thrombosis Mechanisms
- RNA Research and Splicing
- Angiogenesis and VEGF in Cancer
- Chronic Myeloid Leukemia Treatments
- Acute Lymphoblastic Leukemia research
- Hematopoietic Stem Cell Transplantation
- Advanced biosensing and bioanalysis techniques
- Mosquito-borne diseases and control
- Ferroptosis and cancer prognosis
Vanderbilt University Medical Center
2024-2025
YangPu Geriatric Hospital
2025
Tianjin Medical University
2017-2024
Tianjin University
2014-2024
Fudan University Shanghai Cancer Center
2022-2024
First Affiliated Hospital of Henan University
2022-2024
Yunnan University
2019-2024
Ministry of Agriculture and Rural Affairs
2021-2024
Dalian Ocean University
2021-2024
Jilin University
2023
RNA sequencing has become a ubiquitous technology used throughout life sciences as an effective method of measuring abundance quantitatively in tissues and cells. The increase use RNA-seq led to the continuous development new tools for every step analysis from alignment downstream pathway analysis. However, effectively using these scalable reproducible way can be challenging, especially non-experts. Using workflow management system Snakemake we have developed user friendly, fast, efficient,...
Abstract BACKGROUND Metastases to bone are a frequent complication of human prostate cancer and result in the development osteoblastic lesions that include an underlying osteoclastic component. Previous studies rodent models breast have established receptor activator NF‐κB ligand (RANKL) inhibition decreases lesion tumor growth bone. RANK is essential for osteoclast differentiation, activation, survival via its expression on osteoclasts their precursors. has also been observed some cell...
Although immune checkpoint blockade have demonstrated promising results, their effects on gastric cancer (GC) are under investigation. Understanding the clinical significance of PD1 and its ligands' expression, together with T cell infiltration might provide clues for biomarkers screening in GC immunotherapy. Immunohistochemistry were performed a tissue microarray including 1,014 specimens using PD1, PDL1 PDL2 antibodies. markers CD3 CD8 also stained quantified by automated image analysis....
Cancer antigen-specific T cells are key components in antitumor immune response, yet their identification the tumor microenvironment remains challenging, as most cancer antigens unknown. Recent advance immunology suggests that similar T-cell receptor (TCR) sequences can be clustered to infer shared antigen specificity. This study aims identify TCRs from genomics sequencing data.We used TRUST (Tcr Repertoire Utilities for Solid Tissue) algorithm assemble TCR hypervariable CDR3 regions 9,700...
The combination of immune checkpoint blockade and chemotherapy has revolutionized the treatment advanced gastric cancer (GC). It is crucial to unravel chemotherapy-induced tumor microenvironment (TME) modulation identify which immunotherapy would improve antitumor effect.
Synovial fibroblast hyperplasia, T-cell hyperactivity, B-cell overactivation, and the self-perpetuating interactions among these cell types are major characteristics of rheumatoid arthritis (RA). The inflamed joints RA patients hypoxic, with upregulated expression hypoxia-inducible factor-1α (HIF-1α) in synovial fibroblasts (RASFs). It remains unknown whether HIF-1α regulates between RASFs T cells B cells. We report here that promotes inflammatory cytokines IL-6, IL-8, TNF-α, IL-1β,...
Targeting androgen receptor (AR) has shown great therapeutic potential in triple-negative breast cancer (TNBC), yet its efficacy remains unsatisfactory. Here, we aimed to identify promising targeted agents that synergize with enzalutamide, a second-generation AR inhibitor, TNBC. By using strategy for screening drug combinations based on the Sensitivity Index (SI), found MK-8776, selective checkpoint kinase1 (CHK1) showed favorable synergism enzalutamide AR-positive The combination of and...
Aging is associated not only with oxidant stress, but also increased interleukin-6 (IL-6) levels. To determine if oxidative stress could contribute to the age-associated increase IL-6 expression, we exposed LNCaP prostate carcinoma cells and HeLa cervical H2O2 as an challenge. We found that induced IL6 expression through activation of promoter. Furthermore, H2O2-induced promoter was mediated nuclear factor-κB (NFκB) secondary phosphorylation degradation IκBα. NFκB-inducing kinase (NIK)...
Neutrophils are consistently associated with arterial thrombotic morbidity in human clinical studies but the causal basis for this association is unclear. We tested hypothesis that neutrophils modulate platelet activation and thrombus formation vivo a cathepsin G-dependent manner. enhanced aggregation of platelets vitro dose-dependent fashion effect was diminished by pharmacologic inhibition G activity knockdown expression. Tail bleeding time mouse prolonged inhibitor knockout mice,...
// Qingmei He 1, * , Xianyue Ren Jiewei Chen Yingqin Li 1 Xinran Tang Xin Wen Xiaojing Yang Jian Zhang Yaqin Wang Jun Ma Na Liu Sun Yat-Sen University Cancer Center, State Key laboratory of Oncology in South China, Collaborative Innovation Center Medicine, Guangzhou, People’s Republic China These authors contributed equally to this work Correspondence to: Liu, e-mail: liun1@sysucc.org.cn Keywords: miR-16, fibroblast growth factor 2, nasopharyngeal carcinoma, tumor growth, metastasis...
Abstract Background Acute myeloid leukemia (AML), caused by the abnormal proliferation of immature cells in blood or bone marrow, is one most common hematologic malignancies. Currently, interactions between malignant and immune microenvironment, especially T B cells, remain poorly characterized. Methods In this study, we systematically analyzed cell receptor (TCR BCR) repertoires from RNA-seq data 145 pediatric 151 adult AML samples as well 73 non-tumor peripheral samples. Results We...
Solid tumour tissues are composed of and non-tumour cells, such as stromal cells immune cells. These constitute an essential part the microenvironment (TME), which decrease purity play important role in carcinogenesis, malignancy progression, treatment resistance prognostic assessment. However, implications various levels gastric cancer (GC) remain largely unknown. In present study, we used in-silico approach to infer 2,259 GC samples obtained from our hospital 12 public datasets based on...
Studies of human IgE and its targeted epitopes on allergens have been very limited. We an established method to immortalize encoding B cells from allergic individuals. To develop unbiased comprehensive panel peanut-specific mAbs characterize key immunodominant antigenic regions peanut map the molecular interactions responsible for inducing anaphylaxis. Using hybridoma technology peripheral blood subjects with severe allergy, we generated a naturally occurring in manner. Isolated were...
Abstract Background As the objective overall response rate to immune checkpoint inhibitors (ICIs) is less than 30% in late stage or metastatic bladder cancer (BLCA), elucidating intrinsic mechanisms of evasion great importance for discovery predictive and prognostic biomarkers exploration novel targets intervention. Recent studies have shown that OBSCN cytoskeletal protein it encodes, obscurin, play an important role tumour progression. However, no reported BLCA. Methods RNA sequencing...
Abstract Background Thioester-containing proteins (TEPs) serve as crucial effectors and regulatory components within the innate immune system of mosquitoes. Despite their significance, mechanisms by which TEPs exert negative regulation on response in mosquitoes remain inadequately understood. This study aims to elucidate role melanization, thereby advancing our comprehension function response. Methods We infected female Anopheles stephensi with Plasmodium yoelii allowing them feed...