A5101575662- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Immune Cell Function and Interaction
- Cancer Cells and Metastasis
- Cancer Immunotherapy and Biomarkers
- RNA modifications and cancer
- Microbial Metabolism and Applications
- Enzyme-mediated dye degradation
- Cancer Genomics and Diagnostics
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- SARS-CoV-2 and COVID-19 Research
- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- Cytokine Signaling Pathways and Interactions
- Dye analysis and toxicity
- Osteoarthritis Treatment and Mechanisms
- Industrial Technology and Control Systems
- NF-κB Signaling Pathways
- RNA regulation and disease
- Inflammatory Biomarkers in Disease Prognosis
- Oral and Maxillofacial Pathology
- Trypanosoma species research and implications
- Phytochemical compounds biological activities
Anhui University of Science and Technology
2024
Anhui University of Technology
2024
Sun Yat-sen University
2007-2023
Sun Yat-sen University Cancer Center
2012-2023
Soochow University
2012-2023
Sichuan University
2021-2023
Nanfang Hospital
2021-2023
Shanghai First People's Hospital
2016-2023
First Affiliated Hospital of Soochow University
2017-2023
Air Force Medical University
2021-2023
Long non-coding RNA (LncRNA) MEG3 serves a regulatory role in the progression of several types cancer, but laryngeal cancer is still unknown. The aim this study was to explore and mechanism cancer. expression 50 tissue samples detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). effects overexpression on cells were investigated vitro vivo. competitive endogenous (ceRNA) validated through luciferase reporter assay, RT-qPCR Western blotting. down-regulated...
The presence of regulatory T cells in patients who received therapeutic cytokine-induced killer (CIK) may inhibit host immunity, leading to failed immunotherapy. In this study, we investigated the impact using interleukin-6 (IL-6) on phenotype alteration, proliferation, and cytotoxic activity CIK generated from peripheral blood mononuclear with hepatocellular carcinoma. We found that addition IL-6 CIK-cell culture medium decreased percentage Treg/CD4+, Treg/CD3+ resultant simultaneously...
Abstract Ten‐eleven translocation (TET) family enzymes convert 5‐methylcytosine to 5‐hydroxymethylcytosine in DNA. However, the role of TET human gastric cancer remains unknown. Here, we show that TET2 mRNA and protein levels are downregulated tissues when compared with normal mucosa. Low expression predicts poor overall disease‐free survival. Furthermore, demonstrate inhibits proliferation colony formation cells by using loss‐of‐function gain‐of‐function strategies. Overexpression induces...
Abstract Background Acute myeloid leukemia (AML), caused by the abnormal proliferation of immature cells in blood or bone marrow, is one most common hematologic malignancies. Currently, interactions between malignant and immune microenvironment, especially T B cells, remain poorly characterized. Methods In this study, we systematically analyzed cell receptor (TCR BCR) repertoires from RNA-seq data 145 pediatric 151 adult AML samples as well 73 non-tumor peripheral samples. Results We...
Cancer is a disease governed by the underlying gene regulatory networks. The hallmarks of cancer have been proposed to characterize cancerization, e.g., abnormal metabolism, epithelial mesenchymal transition (EMT), and metastasis. We constructed metabolism-EMT-metastasis network quantified its landscape. identified four attractors, characterizing epithelial, metabolic, mesenchymal, metastatic cell states, respectively. Importantly, we an metabolic state. Based on path theory, kinetic paths...
As a newly discovered cytokine, interleukin 9 was initially considered T-lymphocyte growth factor. Interleukin affects target cells by binding to member of the γc-family receptors and is involved in inflammation, autoimmune diseases, other ailments. In recent years, mounting evidence reveals that exerts antitumor effects, which has attracted considerable attention. Many previous studies were performed vivo establishing mouse model melanoma. Here, protein messenger RNA expression levels both...
Cisplatin-based chemotherapy is the first-line treatment for non-small cell lung cancer (NSCLC), but drug resistance occurs in most patients, leading to failure. Recent studies have shown that epithelial–mesenchymal transition (EMT) associated with resistance. However, underlying mechanism not entirely clear. In this study, first we showed significant positive correlation between expression of ERCCl and vimentin, negative E-cadherin neoadjuvant group simple surgery group. Second,...
MicroRNAs (miRNAs) are a class of non‑coding RNAs that play pivotal roles in human lung cancer development. The majority studies have focused on either non-small cell (NSCLC) or small (SCLC). In the present study, we investigated plausible mechanism action miR‑450 these types cancer. We found level was decreased lines, as well solid tumors. As exemplified H510A (SCLC) and H2291 cells, transfection with lentivirus carrying upregulated expression significantly attenuated proliferation...
Objective: To investigate the effect of long noncoding RNA GM16343 on interleukin 36β promotion CD8 + T cells in tumor microenvironment regulation. Methods: The differentially expressed 36β-stimulated mouse was screened by gene chip technology, and significant RNAs were verified real-time polymerase chain reaction. lentiviral vector that overexpresses or knockdown constructed, transfected into cells, stimulated with 36β, amount interferon γ secreted detected enzyme-linked immunosorbent...
Osteoarthritis (OA) is a degenerative disease characterized by cartilage destruction. Previous research has demonstrated that long non‑coding RNAs serve role in OA progression. The current study aimed to determine the function and mechanism of taurine upregulated gene (TUG) 1 OA. results reverse transcription quantitative PCR revealed TUG1 was elevated tissues interleukin (IL)‑1β‑induced chondrocytes. Cell Counting kit‑8 flow cytometry analysis knockdown promoted cell viability inhibited...
Objective: To explore the association of expression leptin and receptor (LR) with bone metastasis pulmonary adenocarcinoma. Methods: One hundred sixteen adenocarcinoma patients who had complete clinicopathological data definite pathological diagnosis in our hospital from January 2008 to 2010 were selected. They divided into (n= 58) non-metastasis (control, n=58) groups. The expressions LR identified by immunohistochemistry. differences between primary tissues metastasis, groups without...
Long non-coding RNAs (LncRNAs) play a critical role in development and progression of various cancers. More more researchers pay attention to the effect lncRNA on regulating cancer. However, function mechanism Duxap8 colorectal cancer have not been studied.Reverse transcription quantitative PCR (RT-qPCR), cell counting kit-8 (CCK-8), 5-ethynyl-20-deoxyuridine (EdU), colony formation assay, flow cytometry, TdT-mediated dUTP nick-end labeling (TUNEL), Western blot, hematoxylin-eosin staining...
To investigate the expression of interleukin-9 (IL-9) in colon cancer tissues and its clinical significance.Immunohistochenmistry qRT-PCR were used to detect expressions IL-9 protein mRNA 92 paired adjacent normal tissues. The correlation with clinicopathological features prognosis patients was analyzed.IL-9 significantly higher than (P < 0.001). In patients, correlated TNM stage (P=0.013), Ducks (P=0.025) lymph node metastasis (P=0.004) but not gender, age, tumor size, differentiation or...
Growing evidence indicates a link between retinoic acid (RA) metabolism and sarcoma progression or immunity in laboratory studies. However, comprehensive analysis of RA abnormality the population is still lacking. Herein, we systematically analyzed molecular features 19 metabolism-related enzymes patients' clinical information based on TCGA/TARGET/GSE datasets. We identified two expression subtypes, which were related to distinct survival outcomes exhibited different biological features....
Non-small-cell lung cancer (NSCLC) is one of the main causes death malignant tumors respiratory system. At present, clinical demand for biomarkers predicting and diagnosing disease increasing. Overexpression LncRNA Hotair (Homeobox transcriptional antisense intergenic RNA) has been previously reported to be associated with poor prognosis high mortality in different malignancies. qRT-PCR results showed that expression tumor tissue serum patients non-small-cell was significantly upregulated....