Nir Hacohen
A5048859270- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Single-cell and spatial transcriptomics
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Cancer Genomics and Diagnostics
- interferon and immune responses
- Immune cells in cancer
- Systemic Lupus Erythematosus Research
- Monoclonal and Polyclonal Antibodies Research
- vaccines and immunoinformatics approaches
- RNA modifications and cancer
- Ferroptosis and cancer prognosis
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Atherosclerosis and Cardiovascular Diseases
- Cancer, Lipids, and Metabolism
- Immune Response and Inflammation
- CRISPR and Genetic Engineering
- Chronic Lymphocytic Leukemia Research
- Renal Diseases and Glomerulopathies
- RNA Interference and Gene Delivery
Massachusetts General Hospital
2016-2025
Broad Institute
2016-2025
Harvard University
2016-2025
Massachusetts Institute of Technology
2014-2025
Dana-Farber Cancer Institute
2013-2025
Center for Cancer Research
2016-2024
Harvard University Press
2011-2024
Boston University
2019-2024
MGH Institute of Health Professions
2022
Ragon Institute of MGH, MIT and Harvard
2021
How the genomic landscape of a tumor shapes and is shaped by anti-tumor immunity has not been systematically explored. Using large-scale data sets solid tissue biopsies, we quantified cytolytic activity local immune infiltrate identified associated properties across 18 types. The number predicted MHC Class I-associated neoantigens was correlated with lower than expected in colorectal other tumors, suggesting immune-mediated elimination. We recurrently mutated genes that showed positive...
What's in a drop of blood? Blood contains many types cells, including immune system components. Immune cells used to be characterized by marker-based assays, but now classification relies on the genes that express. Villani et al. deep sequencing at single-cell level and unbiased clustering define six dendritic cell four monocyte populations. This refined analysis has identified, among others, previously unknown population potently activates T cells. Further culture revealed possible...
The recent advent of methods for high-throughput single-cell molecular profiling has catalyzed a growing sense in the scientific community that time is ripe to complete 150-year-old effort identify all cell types human body. Human Cell Atlas Project an international collaborative aims define terms distinctive profiles (such as gene expression profiles) and connect this information with classical cellular descriptions location morphology). An open comprehensive reference map state cells...
N6-methyladenosine (m6A) is a common modification of mRNA with potential roles in fine-tuning the RNA life cycle. Here, we identify dense network proteins interacting METTL3, component methyltransferase complex, and show that three them (WTAP, METTL14, KIAA1429) are required for methylation. Monitoring m6A levels upon WTAP depletion allowed definition accurate near single-nucleotide resolution methylation maps their classification into WTAP-dependent -independent sites. sites located at...
The somatic genetic basis of chronic lymphocytic leukemia, a common and clinically heterogeneous leukemia occurring in adults, remains poorly understood.
Abstract X chromosome inactivation (XCI) silences transcription from one of the two chromosomes in female mammalian cells to balance expression dosage between XX females and XY males. XCI is, however, incomplete humans: up one-third X-chromosomal genes are expressed both active inactive (Xa Xi, respectively) cells, with degree ‘escape’ varying individuals 1,2 . The extent which is shared tissues remains poorly characterized 3,4 , as does manifests detectable sex differences gene 5 phenotypic...
Abstract Treatment with immune checkpoint blockade (CPB) therapies often leads to prolonged responses in patients metastatic melanoma, but the common mechanisms of primary and acquired resistance these agents remain incompletely characterized have yet be validated large cohorts. By analyzing longitudinal tumor biopsies from 17 melanoma treated CPB therapies, we observed point mutations, deletions or loss heterozygosity (LOH) beta-2-microglobulin ( B2M ), an essential component MHC class I...
Dendritic cells are involved in the initiation of both innate and adaptive immunity. To systematically explore how dendritic modulate immune system response to different pathogens, we used oligonucleotide microarrays measure gene expression profiles Escherichia coli , Candida albicans influenza virus as well their molecular components. Both a shared core pathogen-specific programs were observed upon exposure each these pathogens. These results reveal that sense diverse pathogens elicit...
Antagonists of several growth factor signaling pathways play important roles in developmental patterning by limiting the range cognate inducer. Here, we describe an antagonist FGF that patterns apical branching Drosophila airways. In wild-type embryos, Branchless induces secondary activating Breathless receptor near tips growing primary branches. sprouty mutants, pathway is overactive and ectopic branches are induced on stalks We show expression nearby tip cells, acts nonautonomously a...