A5060241561- Monoclonal and Polyclonal Antibodies Research
- Advanced Proteomics Techniques and Applications
- Advanced Biosensing Techniques and Applications
- Glycosylation and Glycoproteins Research
- RNA and protein synthesis mechanisms
- Protein purification and stability
- Bioinformatics and Genomic Networks
- Bacteriophages and microbial interactions
- Molecular Biology Techniques and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Liver Disease Diagnosis and Treatment
- RNA modifications and cancer
- Metabolomics and Mass Spectrometry Studies
- Biochemical and Structural Characterization
- Single-cell and spatial transcriptomics
- Advanced biosensing and bioanalysis techniques
- Gene expression and cancer classification
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Gut microbiota and health
- Diet and metabolism studies
- Microbial Metabolic Engineering and Bioproduction
- Cancer Genomics and Diagnostics
- Machine Learning in Bioinformatics
- Pancreatic function and diabetes
KTH Royal Institute of Technology
2016-2025
Science for Life Laboratory
2016-2025
Karolinska Institutet
1999-2024
Uppsala University
1981-2024
Stanford University
2024
Novo Nordisk Foundation
2012-2022
Technical University of Denmark
2012-2022
University of Maryland, Baltimore
2022
Yale University
2009-2022
Wallenberg Wood Science Center
2020-2021
Resolving the molecular details of proteome variation in different tissues and organs human body will greatly increase our knowledge biology disease. Here, we present a map tissue based on an integrated omics approach that involves quantitative transcriptomics at organ level, combined with microarray–based immunohistochemistry, to achieve spatial localization proteins down single-cell level. Our tissue-based analysis detected more than 90% putative protein-coding genes. We used this explore...
Global classification of the human proteins with regards to spatial expression patterns across organs and tissues is important for studies biology disease. Here, we used a quantitative transcriptomics analysis (RNA-Seq) classify tissue-specific genes representative set all major combined this antibody-based profiling same tissues. To present data, launch new version Human Protein Atlas that integrates RNA protein data corresponding ∼80% protein-coding access primary both on an individual...
Modeling the cancer transcriptome Recent initiatives such as The Cancer Genome Atlas have mapped genome-wide effect of individual genes on tumor growth. By unraveling genomic alterations in tumors, molecular subtypes cancers been identified, which is improving patient diagnostics and treatment. Uhlen et al. developed a computer-based modeling approach to examine different types nearly 8000 patients. They provide an open-access resource for exploring how expression specific influences...
Mapping the proteome Proteins function in context of their environment, so an understanding cellular processes requires a knowledge protein localization. Thul et al. used immunofluorescence microscopy to map 12,003 human proteins at single-cell level into 30 compartments and substructures (see Perspective by Horwitz Johnson). They validated results mass spectroscopy them model refine protein-protein interaction networks. The is highly spatiotemporally regulated. Many localize multiple...
The recent advent of methods for high-throughput single-cell molecular profiling has catalyzed a growing sense in the scientific community that time is ripe to complete 150-year-old effort identify all cell types human body. Human Cell Atlas Project an international collaborative aims define terms distinctive profiles (such as gene expression profiles) and connect this information with classical cellular descriptions location morphology). An open comprehensive reference map state cells...
Antibody-based proteomics provides a powerful approach for the functional study of human proteome involving systematic generation protein-specific affinity reagents. We used this strategy to construct comprehensive, antibody-based protein atlas expression and localization profiles in 48 normal tissues 20 different cancers. Here we report new publicly available database containing, first version, ∼400,000 high resolution images corresponding more than 700 antibodies toward proteins. Each...
Single-cell RNA analysis has been integrated with spatial protein profiling to create a single–cell type map of human tissues.
Tissue-based diagnostics and research is incessantly evolving with the development of new molecular tools. It has long been realized that immunohistochemistry can add an important level information on top morphology protein expression patterns in a cancer may yield crucial diagnostic prognostic information. We have generated immunohistochemistry-based map profiles normal tissues, cell lines. For each antibody, altogether 708 spots tissues cells are analysed resulting images data presented as...
Spatially resolved transcriptomic technologies are promising tools to study complex biological processes such as mammalian embryogenesis. However, the imbalance between resolution, gene capture, and field of view current methodologies precludes their systematic application analyze relatively large three-dimensional mid- late-gestation embryos. Here, we combined DNA nanoball (DNB)-patterned arrays in situ RNA capture create spatial enhanced resolution omics-sequencing (Stereo-seq). We applied...
Mapping the mammalian brain The diverse physiology of is reflected in its complex organization at regional, cellular, and subcellular levels. Sjöstedt et al. combined data—both newly acquired from other large-scale mapping projects—from transcriptomics, single-cell genomics, situ hybridization, antibody-based protein profiling to map molecular profiles human, pig, mouse brain. analysis consistent with a conserved basic architecture during evolution, but it does show differences regional gene...
Approaches to direct solid phase sequencing of genomic and plasmid DNA have been developed using magnetic beads, coated with streptavidin, as support. The is immobUi2ed through selective incorporation biotin into one the strands. A single stranded template, suitable for sequencing, obtained strand-specific elution. Using this concept, in vitro amplified chromosomal were sequenced directly from colonies. approach ensures that amplification reactions can be performed under optimal conditions....
A synthetic IgG-binding domain based on staphylococcal protein was designed with the aid of sequence comparisons and computer graphic analysis. strategy, utilizing non-palindromic restriction sites, used to overcome difficulties introducing site-specific changes into repetitive gene. single mutagenized gene fragment polymerized different multiplicities, products were expressed in Escherichia coli. Using this scheme, A-like proteins composed numbers domains produced. These changed lack...
Article18 February 2019Open Access Transparent process A deep proteome and transcriptome abundance atlas of 29 healthy human tissues Dongxue Wang orcid.org/0000-0002-4402-0690 Chair Proteomics Bioanalytics, Technische Universität München, Freising, Germany Search for more papers by this author Basak Eraslan Computational Biology, Department Informatics, Technical University Munich, Garching bei Biochemistry, Quantitative Biosciences Gene Center, Ludwig Maximilian Universität, Thomas Wieland...
The gene coding for protein A from Staphylococcus aureus has been isolated by molecular cloning, and a subclone containing an 1.8-kilobase insert was found to give functional in Escherichia coli. complete nucleotide sequence of the insert, including structural 5' 3' flanking sequences, determined. Starting TTG initiator codon, open reading frame comprising 1527 nucleotides gives preprotein 509 amino acids predicted Mr = 58,703. is flanked on both sides palindromic structures followed stretch...
The large vascular meristem of poplar trees with its highly organized secondary xylem enables the boundaries between different developmental zones to be easily distinguished. This property wood-forming tissues allowed us determine a unique tissue-specific transcript profile for well defined gradient. RNA was prepared from stages xylogenesis DNA microarray analysis by using hybrid aspen unigene set consisting 2,995 expressed sequence tags. revealed that genes encoding lignin and cellulose...