A5031060352- Chemical Synthesis and Analysis
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Click Chemistry and Applications
- Biochemical and Structural Characterization
- Monoclonal and Polyclonal Antibodies Research
- Cancer-related gene regulation
- Protein Degradation and Inhibitors
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Peptidase Inhibition and Analysis
- Antimicrobial Resistance in Staphylococcus
- Glycosylation and Glycoproteins Research
- Bacterial biofilms and quorum sensing
- RNA Research and Splicing
- History and Theory of Mathematics
- Protein Structure and Dynamics
- Mathematics and Applications
- Antimicrobial Peptides and Activities
- Enzyme Structure and Function
- Biotin and Related Studies
- Chromatin Remodeling and Cancer
- Microbial Natural Products and Biosynthesis
- Advanced biosensing and bioanalysis techniques
Princeton University
2016-2025
Methodist Hospital
2020-2022
The University of Texas Health Science Center at Houston
2022
Princeton Public Schools
2016-2021
OsloMet – Oslo Metropolitan University
2018
Rockefeller University
2003-2013
The University of Texas at Austin
2013
Tri-Institutional PhD Program in Chemical Biology
2008-2011
Cornell University
2003-2011
Active Motif (United States)
2010
A simple technique has been devised that allows the direct synthesis of native backbone proteins moderate size. Chemoselective reaction two unprotected peptide segments gives an initial thioester-linked species. Spontaneous rearrangement this transient intermediate yields a full-length product with bond at ligation site. The utility chemical was demonstrated by one-step preparation cytokine containing multiple disulfides. polypeptide folded and oxidized to form disulfide-containing protein...
Sequencing of pediatric gliomas has identified missense mutations Lys27Met (K27M) and Gly34Arg/Val (G34R/V) in genes encoding histone H3.3 (H3F3A) H3.1 (HIST3H1B). We report that human diffuse intrinsic pontine (DIPGs) containing the K27M mutation display significantly lower overall amounts H3 with trimethylated lysine 27 (H3K27me3) H3K27M transgenes are sufficient to reduce H3K27me3 vitro vivo. find inhibits enzymatic activity Polycomb repressive complex 2 through interaction EZH2 subunit....
A protein semisynthesis method—expressed ligation—is described that involves the chemoselective addition of a peptide to recombinant protein. This method was used ligate phosphotyrosine C terminus tyrosine kinase C-terminal Src (Csk). By intercepting thioester generated in with an N-terminal cysteine containing synthetic peptide, near quantitative chemical ligation achieved. The semisynthetic tail-phosphorylated Csk showed evidence intramolecular phosphotyrosine-Src homology 2 interaction...
The synthesis of virulence factors and other extracellular proteins responsible for pathogenicity in Staphylococcus aureus is under the control agr locus. A secreted agr-encoded peptide, AgrD, processed from AgrD gene product, known to be an effector self-strain activation cross-strain inhibition response. Biochemical analysis peptides isolated culture supernatants has suggested that they contain unusual thiol ester-linked cyclic structure. In present work, chemical used confirm mature a...
An oncohistone deranges inhibitory chromatin Missense mutations (that change one amino acid for another) in histone H3 can produce a so-called and are found number of pediatric cancers. For example, the lysine-36–to-methionine (K36M) mutation is seen almost all chondroblastomas. Lu et al. show that K36M mutant histones oncogenic, they inhibit normal methylation this same residue wild-type histones. The also interfere with development bone-related cells deposition marks. Science , issue p. 844
Inteins are auto-processing domains found in organisms from all of life. These proteins carry out a process known as protein splicing, which is multi-step biochemical reaction comprised both the cleavage and formation peptide bonds. While endogenous substrates splicing specific essential intein-containing host organisms, inteins also functional exogenous contexts can be used to chemically manipulate virtually any polypeptide backbone. Given this, chemists have exploited various facets intein...
Abstract Posterior fossa type A (PFA) ependymomas exhibit very low H3K27 methylation and express high levels of EZHIP (Enhancer Zeste Homologs Inhibitory Protein, also termed CXORF67 ). Here we find that a conserved sequence in is necessary sufficient to inhibit PRC2 catalytic activity vitro vivo. directly contacts the active site EZH2 subunit mechanism similar H3 K27M oncohistone. Furthermore, expression or cells promotes chromatin profiles: loss broad H3K27me3 domains, but retention at CpG...
Abstract Histone H3 monoaminylations at Gln5 represent an important family of epigenetic marks in brain that have critical roles permissive gene expression 1–3 . We previously demonstrated serotonylation 4–10 and dopaminylation 9,11–13 histone (H3Q5ser H3Q5dop, respectively) are catalysed by transglutaminase 2 (TG2), alter both local global chromatin states. Here we found TG2 additionally functions as eraser exchanger monoaminylations, including H3Q5 histaminylation (H3Q5his), which displays...