A5029331756- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- RNA modifications and cancer
- RNA Research and Splicing
- Glioma Diagnosis and Treatment
- Protein Degradation and Inhibitors
- Ubiquitin and proteasome pathways
- Histone Deacetylase Inhibitors Research
- RNA Interference and Gene Delivery
- Chromatin Remodeling and Cancer
- RNA and protein synthesis mechanisms
- Acute Myeloid Leukemia Research
- Plant Molecular Biology Research
- Cancer Genomics and Diagnostics
- Fungal and yeast genetics research
- Cancer, Hypoxia, and Metabolism
- Genetics and Neurodevelopmental Disorders
- Chromosomal and Genetic Variations
- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- Glycosylation and Glycoproteins Research
- Immune Cell Function and Interaction
- Cancer-related molecular mechanisms research
- ATP Synthase and ATPases Research
Northwestern University
2016-2025
Shandong University
2022
Institute for Medical Research
2022
Northwestern University
2022
Northwestern Memorial Hospital
2020
University of Kansas
2019
National Institutes of Health
2002-2019
Leidos (United States)
2019
Leidos Biomedical Research Inc. (United States)
2019
Tufts University
2019
A recent meeting (December 2008) regarding chromatin-based epigenetics was hosted by the Banbury Conference Center and Cold Spring Harbor Laboratory. The intent to discuss aspects of epigenetic control genomic function, arrive at a consensus definition "epigenetics" be considered broader community. It evident that multiple mechanistic steps lead stable heritance phenotype. Below we provide our view interpretation proceedings meeting.
The contributions of diverse cell populations in the human lung to pulmonary fibrosis pathogenesis are poorly understood. Single-cell RNA sequencing can reveal changes within individual during that important for disease pathogenesis.
Little is known about the relative importance of monocyte and tissue-resident macrophages in development lung fibrosis. We show that specific genetic deletion monocyte-derived alveolar after their recruitment to ameliorated fibrosis, whereas did not contribute Using transcriptomic profiling flow-sorted cells, we found macrophage differentiation unfolds continuously over course fibrosis its resolution. During fibrotic phase, differ significantly from expression profibrotic genes. A population...
Gene activation and repression regulated by acetylation deacetylation represent a paradigm for the function of histone modifications. We provide evidence that, in contrast, H2B monoubiquitylation its deubiquitylation are both involved gene activation. Substitution ubiquitylation site at Lys 123 (K123) lowered transcription certain genes complex SAGA. Gene-associated was transient, increasing early during activation, then decreasing coincident with significant RNA accumulation. show that...
Set2 methylates Lys36 of histone H3. We show here that yeast copurifies with RNA polymerase II (RNAPII). Chromatin immunoprecipitation analyses demonstrated and H3 methylation are associated the coding regions several genes were tested correlate active transcription. Both depend, as well, on Paf1 elongation factor complex. The C terminus Set2, which contains a WW domain, is also required for effective methylation. Deletion CTK1, encoding an RNAPII CTD kinase, prevents recruitment, suggesting...
The trithorax genes encode an evolutionarily conserved family of proteins that function to maintain specific patterns gene expression throughout cellular development. Members this protein contain a highly 130- 140-amino acid motif termed the SET domain. We report purification and molecular identification subunits complex in yeast Saccharomyces cerevisiae includes trithorax-related Set1. This complex, which we have named COMPASS (Complex Proteins Associated with Set1), consists seven...
The DNA of eukaryotes is wrapped around nucleosomes and packaged into chromatin. Covalent modifications the histone proteins that comprise nucleosome alter chromatin structure have major effects on gene expression. Methylation lysine 4 H3 by COMPASS required for silencing genes located near chromosome telomeres within rDNA (Krogan, N. J, Dover, J., Khorrami, S., Greenblatt, J. F., Schneider, Johnston, M., Shilatifard, A. (2002) Biol. Chem. 277, 10753-10755; Briggs, S. D., Bryk, Strahl, B....
To physically characterize the web of interactions connecting Saccharomyces cerevisiae proteins suspected to be RNA polymerase II (RNAPII) elongation factors, subunits Spt4/Spt5 and Spt16/Pob3 (corresponding human DSIF FACT), Spt6, TFIIF (Tfg1, -2, -3), TFIIS, Rtf1, Elongator (Elp1, -3, -4, -5, -6) were affinity purified under conditions designed minimize loss associated polypeptides then identified by mass spectrometry. was discovered associate with three distinct complexes: histones;...