A5068855164- Bioinformatics and Genomic Networks
- Respiratory Support and Mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Congenital heart defects research
- Fungal and yeast genetics research
- Protein Structure and Dynamics
- Sepsis Diagnosis and Treatment
- Genetics, Aging, and Longevity in Model Organisms
- Cardiomyopathy and Myosin Studies
- Mitochondrial Function and Pathology
- Intensive Care Unit Cognitive Disorders
- COVID-19 Clinical Research Studies
- Renal and related cancers
- Metabolomics and Mass Spectrometry Studies
- Tracheal and airway disorders
- Epigenetics and DNA Methylation
- Long-Term Effects of COVID-19
- Cancer-related molecular mechanisms research
- Zebrafish Biomedical Research Applications
- ATP Synthase and ATPases Research
- Gene expression and cancer classification
- Cardiovascular Effects of Exercise
- MicroRNA in disease regulation
- Congenital Heart Disease Studies
- Tuberous Sclerosis Complex Research
Centro Científico Tecnólogico - Rosario
2022-2024
Brigham and Women's Hospital
2012-2022
Harvard University
2013-2022
Harvard Stem Cell Institute
2013-2020
University of Toronto
2005-2010
Canada Research Chairs
2007-2008
Hospital for Sick Children
2005
SickKids Foundation
2005
University of Bologna
2001
One-third of the 4,225 protein-coding genes Escherichia coli K-12 remain functionally unannotated (orphans). Many map to distant clades such as Archaea, suggesting involvement in basic prokaryotic traits, whereas others appear restricted E. coli, including pathogenic strains. To elucidate orphans' biological roles, we performed an extensive proteomic survey using affinity-tagged strains and generated comprehensive genomic context inferences derive a high-confidence compendium for virtually...
Abstract Parasitic nematodes infect one quarter of the world’s population and impact all humans through widespread infection crops livestock. Resistance to current anthelmintics has prompted search for new drugs. Traditional screens that rely on parasitic worms are costly labour intensive target-based approaches have failed yield novel anthelmintics. Here, we present our screen 67,012 compounds identify those kill non-parasitic nematode Caenorhabditis elegans . We then rescreen hits in two...
The vertebrate heart muscle (myocardium) develops from the first field (FHF) and expands by adding second (SHF) cells. While both lineages exist already in teleosts, primordial contributions of FHF SHF to structure function remain incompletely understood. Here we delineate functional contribution zebrafish using cis-regulatory elements draculin (drl) gene. drl reporters initially lateral plate mesoderm, including progenitors. Subsequent myocardial reporter expression restricts descendants....
Since complete redundancy between extant duplicates (paralogs) is evolutionarily unfavorable, some degree of functional congruency eventually lost. However, in budding yeast, experimental evidence collected for duplicated metabolic enzymes and global physical interaction surveys had suggested widespread overlap paralogs. While maintained thought to confer robustness against genetic mutation facilitate environmental adaptability, it has yet be determined what properties define paralogs that...
Highlights•Rewiring of mitochondrial (mt) protein interaction network in distinct cell states•Dramatic changes site-specific phosphorylation during neuronal differentiation•C20orf24 is a respirasome assembly factor depleted patients deficient CIV•NENF binding with DJ-1/PINK1 promotes neurotrophic activity and survivalSummaryMitochondrial (MP) assemblies undergo alterations neurogenesis, complex process vital brain homeostasis disease. Yet which MP remodel differentiation remains unclear....
As antibiotic resistance is increasingly becoming a public health concern, an improved understanding of the bacterial DNA damage response (DDR), which commonly targeted by antibiotics, could be tremendous therapeutic value. Although genetic components DDR have been studied extensively in isolation, how underlying biological pathways interact functionally remains unclear. Here, we address this performing systematic, unbiased, quantitative synthetic interaction (GI) screens and uncover...
Pancreatic cancer consists of a heterogenous bulk tumor cells and stroma which contribute to progression by releasing angiogenic factors. Those factors can be detected as circulating serum We performed compartment-specific analysis tumor-derived stroma-derived identify biomarkers molecular targets for the treatment pancreatic cancer. Kryo-frozen tissue from primary ductal adenocarcinomas (n = 51) was laser-microdissected isolate tissue. Expression 17 (angiopoietin-2, follistatin, GCSF, HGF,...
Cardiovascular disease (CVD) is the leading cause of death in developed world. Human genetic studies, including genome-wide sequencing and SNP-array approaches, promise to reveal genes mechanisms representing new therapeutic targets. In practice, however, identification actual contributing pathogenesis has lagged behind associated loci, thus limiting clinical benefits.
Emerging technologies now allow for mass spectrometry-based profiling of thousands small molecule metabolites ('metabolomics') in an increasing number biosamples. While offering great promise insight into the pathogenesis human disease, standard approaches have not yet been established statistically analyzing increasingly complex, high-dimensional metabolomics data relation to clinical phenotypes, including disease outcomes. To determine optimal analysis, we formally compare traditional and...
Although recent advances have been made in identifying and analyzing instances of microRNA-mediated gene regulation, it remains unclear by what mechanisms attenuation transcript expression through microRNAs becomes an integral part post-transcriptional modification, is even less clear to extent this process occurs for mammalian duplicates (paralogs). Specifically, while paralogs are known overcome their initial complete functional redundancy variation regulation expression, the potential...
Comprehensive functional annotation of vertebrate genomes is fundamental to biological discovery. Reverse genetic screening has been highly useful for determination gene function, but untenable as a systematic approach in model organisms given the number surveyable genes and observable phenotypes. Unbiased prediction gene-phenotype relationships offers strategy direct finite experimental resources towards likely phenotypes, thus maximizing de novo discovery functions. Here we prioritized...
Although detailed, focused, and mechanistic analyses of associations among mitochondrial proteins (MPs) have identified their importance in varied biological processes, a systematic understanding how MPs function concert both with one another extra-mitochondrial remains incomplete. Consequently, many questions regarding the role dysfunction development human disease remain unanswered. To address this, we compiled all existing physical interaction data for over 1200 experimentally defined...
Acute Respiratory Distress Syndrome (ARDS) is associated with high morbidity and mortality. Identification of ARDS enables lung protective strategies, quality improvement interventions, clinical trial enrolment, but remains challenging particularly in the first 24 hours mechanical ventilation. To address this we built an algorithm capable discriminating from other similarly presenting disorders immediately following Specifically, a team examined medical records 1263 ICU-admitted,...