A5101881010- Zebrafish Biomedical Research Applications
- Epigenetics and DNA Methylation
- Hemoglobinopathies and Related Disorders
- RNA modifications and cancer
- Congenital heart defects research
- Acute Myeloid Leukemia Research
- Genomics and Chromatin Dynamics
- Erythrocyte Function and Pathophysiology
- MicroRNA in disease regulation
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Aquaculture disease management and microbiota
- Muscle Physiology and Disorders
- Neurobiology and Insect Physiology Research
- Developmental Biology and Gene Regulation
- DNA Repair Mechanisms
- Angiogenesis and VEGF in Cancer
- Cancer-related gene regulation
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Cellular transport and secretion
- Single-cell and spatial transcriptomics
- interferon and immune responses
- Hematopoietic Stem Cell Transplantation
- Microtubule and mitosis dynamics
Jinhua Academy of Agricultural Sciences
2025
Boston Children's Hospital
2014-2024
Harvard University
2014-2024
Howard Hughes Medical Institute
2013-2024
Harvard Stem Cell Institute
2012-2024
Dana-Farber Cancer Institute
2014-2024
Shaanxi University of Science and Technology
2024
Henan Agricultural University
2024
Hubei University of Arts and Science
2022-2024
Xiangyang Central Hospital
2022-2024
A high-quality sequence assembly of the zebrafish genome reveals largest gene set any vertebrate and provides information on key genomic features, comparison to human reference shows that approximately 70% protein-coding genes have at least one clear orthologue. The — a model organism for study development disease has now been sequenced published as well-annotated genome. Zebrafish turns out so far sequenced, few pseudogenes. Importantly studies, between sequences obvious second paper...
Xiaowen Sun and colleagues report the whole-genome sequencing of common carp, Cyprinus carpio. They also resequenced 33 representative accessions from a worldwide collection provide insights into population structure evolution. The carpio, is one most important cyprinid species globally accounts for 10% freshwater aquaculture production. Here we present draft genome domesticated C. carpio (strain Songpu), whose current assembly contains 52,610 protein-coding genes approximately 92.3%...
Mutations in the gene parkin humans (PARK2) are responsible for a large number of familial cases autosomal-recessive Parkinson disease. We have isolated Drosophila homolog human PARK2 and characterized its expression null phenotype. flies 30% lower mass than wild-type controls which is part accounted by reduced cell size number. In addition, these infertile, show significantly longevity, unable to jump or fly. Rearing mutants on paraquat, generates toxic free radicals vivo, causes further...
African genomics and skin color Skin varies among human populations is thought to be under selection, with light maximizing vitamin D production at higher latitudes dark providing UV protection in equatorial zones. To identify the genes that give rise palette of tones, Crawford et al. applied genome-wide analyses across diverse (see Perspective by Tang Barsh). Genetic variants were identified likely function phenotypes. Comparison model organisms verified a conserved MFSD12 pigmentation. A...
5' strand resection at DNA double breaks (DSBs) is critical for homologous recombination (HR) and genomic stability. Here we develop a novel method to quantitatively measure single-stranded intermediates in human cells find that the endonuclease-generated break sites resected up 3.5 kb cell cycle-dependent manner. Depletion of CtIP, Mre11, Exo1 or SOSS1 blocks resection, while depletion 53BP1, Ku DNA-dependent protein kinase catalytic subunit leads increased as measured by this method. While...
Gfi1 was first identified as causing interleukin 2-independent growth in T cells and lymphomagenesis mice. Much work has shown thatGfi1 Gfi1b, a second mouse homolog, play pivotal roles blood cell lineage differentiation. However, neither norGfi1b been implicated nervous system development, even though their invertebrate homologues, senseless Drosophila andpag-3 C. elegans are expressed required the system. We show that mRNA is many areas give rise to neuronal during embryonic development...
The van gogh (vgo) mutant in zebrafish is characterized by defects the ear, pharyngeal arches and associated structures such as thymus. We show that vgo caused a mutation tbx1, member of large family T-box genes. tbx1 has been recently suggested to be major contributor cardiovascular DiGeorge deletion syndrome (DGS) humans, which several neural crest derivatives are affected arches. Using cell transplantation studies, we demonstrate vgo/tbx1 acts autonomously mesendoderm influences...
Recent studies have implicated a poorly defined alternative pathway of nonhomologous end joining (alt-NHEJ) in the generation large deletions and chromosomal translocations that are frequently observed cancer cells. Here, we describe an interaction between two factors, hMre11/hRad50/Nbs1 (MRN) DNA ligase IIIα/XRCC1, been linked with alt-NHEJ. Expression IIIα association MRN IIIα/XRCC1 altered cell lines defective major NHEJ pathway. Most notably, damage induced these factors IV-deficient...
The vertebrate heart muscle (myocardium) develops from the first field (FHF) and expands by adding second (SHF) cells. While both lineages exist already in teleosts, primordial contributions of FHF SHF to structure function remain incompletely understood. Here we delineate functional contribution zebrafish using cis-regulatory elements draculin (drl) gene. drl reporters initially lateral plate mesoderm, including progenitors. Subsequent myocardial reporter expression restricts descendants....
Congenital muscular dystrophy (CMD) is a clinically and genetically heterogeneous group of inherited muscle disorders. In patients, weakness usually present at or shortly after birth progressive in nature. Merosin deficient congenital (MDC1A) form CMD caused by defect the laminin-α2 gene (LAMA2). Laminin-α2 an extracellular matrix protein that interacts with dystrophin-dystroglycan (DGC) complex membranes providing stability to fibers. N-ethyl-N-nitrosourea mutagenesis screen develop...
Independent functions in the response to DNA damage and oxidative stress broaden biological roles of kinase ATM.
Abstract MicroRNA-146a is upregulated in the brains of patients with Alzheimer’s disease (AD). Here, we show that rho-associated, coiled-coil containing protein kinase 1 (ROCK1) a target microRNA-146a neural cells. Knockdown ROCK1 mimicked effects overexpression and induced abnormal tau phosphorylation, which was associated inhibition phosphorylation phosphatase tensin homolog (PTEN). The ROCK1/PTEN pathway has been implicated neuronal hyperphosphorylation occurs AD. To determine function...
Significance Exonuclease 1 (Exo1) is a conserved eukaryotic nuclease that participates in DNA repair and telomere maintenance. Here we use high-throughput single-molecule imaging to examine Exo1 activity on the presence of single-stranded binding proteins. We report both human yeast are processive nucleases but rapidly turned over by replication protein A (RPA). In RPA, retains limited DNA-processing activity, albeit via distributive mechanism. This rapid turnover RPA can appear stimulatory...
A major goal of cancer research has been to identify genes that contribute formation. The similar pathology between zebrafish and human tumors, as well the past success large-scale genetic screens in uncovering disease genes, makes an ideal system which find such new genes. Here, we show a forward screen uncovered multiple cell proliferation mutants including one mutant, crash&burn ( crb ), represents loss-of-function mutation bmyb , transcriptional regulator member putative...