A5061807667- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- RNA modifications and cancer
- Cancer Genomics and Diagnostics
- Systemic Lupus Erythematosus Research
- Cancer Cells and Metastasis
- Ferroptosis and cancer prognosis
- Immune Cell Function and Interaction
- Cancer-related molecular mechanisms research
- Renal and related cancers
- Estrogen and related hormone effects
- Salivary Gland Disorders and Functions
- Pancreatic and Hepatic Oncology Research
- Cancer, Hypoxia, and Metabolism
- Erythrocyte Function and Pathophysiology
- Iron Metabolism and Disorders
- Autophagy in Disease and Therapy
- Mitochondrial Function and Pathology
- Hemoglobinopathies and Related Disorders
- Glycosylation and Glycoproteins Research
- T-cell and B-cell Immunology
- Acute Myeloid Leukemia Research
- Metabolism and Genetic Disorders
- Liver Disease Diagnosis and Treatment
- Endoplasmic Reticulum Stress and Disease
Tohoku University
2015-2024
Tohoku Medical Megabank Organization
2014-2020
Hirose Electric (Japan)
2015-2018
Hiroshima University
2016
National Defense Medical College
1999-2014
Kitasato University
2002-2005
Tokyo National Hospital
1998
Japan Association for Development of Community Medicine
1992
Jikei University School of Medicine
1962
S-adenosylmethionine (SAM) is an important metabolite as a methyl-group donor in DNA and histone methylation, tuning regulation of gene expression. Appropriate intracellular SAM levels must be maintained, because methyltransferase reaction rates can limited by availability. In response to depletion, MAT2A, which encodes ubiquitous mammalian methionine adenosyltransferase isozyme, was upregulated through mRNA stabilization. SAM-depletion reduced N6-methyladenosine (m6A) the 3′ UTR MAT2A....
Ferroptosis is an iron-dependent programmed cell death event, whose regulation and physiological significance remain to be elucidated. Analyzing transcriptional responses of mouse embryonic fibroblasts exposed the ferroptosis inducer erastin, here we found that a set genes related oxidative stress protection induced upon ferroptosis. We considered up-regulation these attenuates induction transcription factor BTB domain CNC homolog 1 (BACH1), regulator in heme iron metabolism, promotes by...
We examined the competition of binding Lactobacillus reuteri and Helicobacter pylori to gangliotetraosylceramide (asialo-GM1) sulfatide which are putative glycolipid receptor molecules H. pylori, identified a possible sulfatide-binding protein L. strain. Among nine strains, two (JCM1081 TM105) were shown bind asialo-GM1 sulfatide, inhibit both glycolipids by thin layer chromatogram-overlay assay using biotin-labeled bacterial cells. The extract from cells strain TM105 with several...
Abstract Pancreatic ductal adenocarcinoma (PDAC) is among the cancers with poorest prognoses due to its highly malignant features. BTB and CNC homology 1 (BACH1) has been implicated in RAS-driven tumor formation. We focused on role of BACH1 PDAC, more than 90% which have KRAS mutation. Knockdown PDAC cell lines reduced migration invasion, part, by increasing E-cadherin expression, whereas overexpression showed opposite effects. directly repressed expression FOXA1 that known activate CDH1...
Abstract Ferroptosis is a regulated cell death due to the iron-dependent accumulation of lipid peroxide. known constitute pathology ischemic diseases, neurodegenerative and steatohepatitis also works as suppressing mechanism against cancer. However, how ferroptotic cells affect surrounding remains elusive. We herein report transfer phenomenon peroxidation from nearby that are not exposed inducers (FINs). While primary mouse embryonic fibroblasts (MEFs) NIH3T3 contained senescence-associated...
Parkinson's disease (PD) is a progressive neurodegenerative movement disorder characterized by the loss of nigrostriatal dopaminergic neurons. Mounting evidence suggests that Nrf2 promising target for neuroprotective interventions in PD. However, electrophilic chemical properties canonical Nrf2-based drugs cause irreversible alkylation cysteine residues on cellular proteins resulting side effects. Bach1 known transcriptional repressor pathway. We report levels are up-regulated PD postmortem...
Significance Primordial germ cells (PGCs) are the origin of and critically important for continuity multicellular organisms. Although unique characteristics mouse PGCs have been described in gene expression epigenome levels, metabolomic proteomic profiles their significance PGC properties unclear. Our findings this study demonstrate not only distinct energy metabolisms pluripotent stem (PSCs), but also essential contribution enhanced oxidative phosphorylation glycolysis specification from...
Hematopoietic stem cell and multipotent progenitor (MPP) commitment can be tuned in response to an infection so that their differentiation is biased toward myeloid cells. Here, we find Bach2, which inhibits common lymphoid progenitors, represses a cohort of genes activates those linked function. Bach2 repressed both Cebpb its target Csf1r, encoding C/EBPβ macrophage colony-stimulating factor receptor (M-CSFr), respectively, whereas activated Csf1r. further bound overlapping regulatory...
Ferroptosis is a regulated cell death induced by iron-dependent lipid peroxidation. The heme-responsive transcription factor BTB and CNC homology 1 (BACH1) promotes ferroptosis repressing the of genes involved in glutathione (GSH) synthesis intracellular labile iron metabolism, which are key regulatory pathways ferroptosis. We found that BACH1 re-expression Bach1-/- immortalized mouse embryonic fibroblasts (iMEFs) can induce upon 2-mercaptoethanol removal, without any inducers. In these...
Ferroptosis is a type of regulated cell death characterized by iron-dependent lipid peroxidation. A model system constructed to induce ferroptosis re-expressing the transcription factor BACH1, potent inducer, in immortalized mouse embryonic fibroblasts (iMEFs). The transfer culture supernatant from ferroptotic iMEFs activates proliferation hepatoma cells and other suppresses cellular senescence-like features. BACH1-dependent secretion longevity FGF21 increased iMEFs. anti-senescent effects...
Head and neck squamous cell carcinoma (HNSCC) remains difficult to treat due the lack of molecularly targeted drugs with high anti-tumor efficacy safety. To investigate involvement transcription factor BACH1, which is known promote metastasis various cancers, in malignant properties HNSCC cells, we examined effects BACH1 depletion short interfering RNAs human lines. We found that knockdown induced death intracellular labile iron not tightly bound protein. Mitochodrial electron transfer chain...
<title>Abstract</title> The nucleolus is the site of rDNA transcription and ribosome biogenesis. Alterations in nucleolar function architecture correlate with drastic heterochromatin rearrangement global changes gene expression. However, precise mechanism that connects to organization yet unknown. Here, we report RNA polymerase II (RNA pol II) transactivator chromatin condenser, Positive Coactivator 4 (PC4), a bona fide protein. PC4 showed dynamic accumulation, which critical for...
The transcription factor Bach2 regulates both acquired and innate immunity at multiple steps, including antibody class switching regulatory T cell development in activated B cells, respectively. However, little is known about the molecular mechanisms of regulation response to signaling cytokines antigen. We show here that mammalian target rapamycin (mTOR) controls along differentiation with two distinct pre-B cells. First, mTOR complex 1 (mTORC1) inhibited accumulation protein nuclei reduced...
BTB and CNC homology 1 (BACH1) represses the expression of genes involved in metabolism iron, heme reactive oxygen species. While BACH1 is rapidly degraded when it bound to heme, remains unclear how degradation regulated under other conditions. We found that FBXO22, a ubiquitin ligase previously reported promote degradation, polyubiquitinated only presence highly purified reconstitution assay. In parallel this regulatory mechanism, TANK binding kinase (TBK1), protein activates innate immune...
The transcription repressor BTB and CNC homology 1 ( BACH 1) represses genes involved in heme metabolism oxidative stress response. also suppresses the p53‐dependent cellar senescence primary mouse embryonic fibroblasts MEF s). To investigate role of other than its known functions, we carried out a genomewide mapping binding site for heterodimer partner MAFK immortalized s iMEF s) using chromatin immunoprecipitation next‐generation sequencing technology (Ch IP ‐sequence). comparative...
Abstract BTB and CNC homology 2 (Bach2) is a transcriptional repressor that required for the formation of germinal center (GC) reactions, including class switch recombination somatic hypermutation Ig genes in B cells, within GC. Although BCR-induced proliferation essential GC function Bach2 regulating cell has not been elucidated. In this study, we demonstrate to sustain high levels response BCR signaling. Following engagement vitro, cells from Bach2-deficient (Bach2−/−) mice showed lower...
The transcription repressor BACH1 performs mutually independent dual roles in regulation and chromosome alignment during mitosis by supporting polar ejection force of mitotic spindle. We now found that the spindles became oblique relative to adhesion surface following endogenous depletion HeLa cells. This spindle orientation rearrangement was rescued re-expression depending on its interactions with HMMR CRM1, both which are required for positioning spindle, but independently DNA-binding...