A5013616060- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Genomics and Chromatin Dynamics
- Heme Oxygenase-1 and Carbon Monoxide
- Epigenetics and DNA Methylation
- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- RNA Research and Splicing
- Cytokine Signaling Pathways and Interactions
- RNA modifications and cancer
- Erythrocyte Function and Pathophysiology
- Developmental Biology and Gene Regulation
- interferon and immune responses
- Cancer-related molecular mechanisms research
- Chronic Lymphocytic Leukemia Research
- Retinoids in leukemia and cellular processes
- Chronic Myeloid Leukemia Treatments
- Cancer-related gene regulation
- Zebrafish Biomedical Research Applications
- Lymphoma Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Single-cell and spatial transcriptomics
- RNA Interference and Gene Delivery
- Acute Myeloid Leukemia Research
- Genomics, phytochemicals, and oxidative stress
Tohoku University
2013-2024
Kanazawa University
2024
Hiroshima University
2001-2018
Japan Agency for Medical Research and Development
2016-2017
Japan Science and Technology Agency
2013-2016
University of California, Irvine
2009-2014
Respiratory Clinical Trials
2013
The University of Tokyo
1992-2009
Tokyo University of Science
1993-2009
University of Tsukuba
1998-2000
Chromatin reorganization plays an important role in DNA repair, apoptosis, and cell cycle checkpoints. Among proteins involved chromatin reorganization, TIP60 histone acetyltransferase has been shown to play a repair apoptosis. However, how regulates the response of human cells damage is largely unknown. Here, we show that ionizing irradiation induces acetylation H2AX, variant form H2A known be phosphorylated following damage. Furthermore, ubiquitination H2AX via ubiquitin-conjugating enzyme...
S-adenosylmethionine (SAM) is an important metabolite as a methyl-group donor in DNA and histone methylation, tuning regulation of gene expression. Appropriate intracellular SAM levels must be maintained, because methyltransferase reaction rates can limited by availability. In response to depletion, MAT2A, which encodes ubiquitous mammalian methionine adenosyltransferase isozyme, was upregulated through mRNA stabilization. SAM-depletion reduced N6-methyladenosine (m6A) the 3′ UTR MAT2A....
The human β-globin locus control region (LCR) is required to properly regulate chromatin domain opening, replication timing, and globin gene activation. LCR contains multiple NF-E2 sites (<i>Ma</i>f <i>r</i>ecognition <i>e</i>lements, MAREs) that allow the binding of various basic leucine zipper (bZip) proteins like p45 NF-E2, Nrf1, Nrf2, Bach1, Bach2, in some cases as obligate heterodimers with a small Maf protein. In addition bZip domain, Bach bear BTB/POZ which has been implicated...
During immune reactions, functionally distinct B-cell subsets are generated by stochastic processes, including class-switch recombination (CSR) and plasma cell differentiation (PCD). In this study, we show a strong association between individual fates mitochondrial functions. CSR occurs specifically in activated B cells with increased mass membrane potential, which augment reactive oxygen species (mROS), whereas PCD decreased potential. These events consequences of initial slight changes...
The transcriptional repressor BTB and CNC homology 2 (Bach2) is thought to be mainly expressed in B cells with specific functions such as class switch recombination somatic hypermutation, but its function T not known. We found equal Bach2 expression analyzed using Bach2-deficient (-/-) mice. Although T-cell development was normal, numbers of peripheral naive were decreased, which rapidly produced Th2 cytokines after TCR stimulation. Bach2(-/-) highly genes related effector-memory CCR4, ST-2...
Pulmonary alveolar proteinosis (PAP) results from a dysfunction of macrophages (AMs), chiefly due to disruptions in the signaling granulocyte macrophage colony–stimulating factor (GM-CSF). We found that mice deficient for B lymphoid transcription repressor BTB and CNC homology 2 (Bach2) developed PAP-like accumulation surfactant proteins lungs. Bach2 was expressed AMs, Bach2-deficient AMs showed alterations lipid handling comparison with wild-type (WT) cells. Although normal expression genes...
Abstract Ferroptosis is a regulated cell death due to the iron-dependent accumulation of lipid peroxide. known constitute pathology ischemic diseases, neurodegenerative and steatohepatitis also works as suppressing mechanism against cancer. However, how ferroptotic cells affect surrounding remains elusive. We herein report transfer phenomenon peroxidation from nearby that are not exposed inducers (FINs). While primary mouse embryonic fibroblasts (MEFs) NIH3T3 contained senescence-associated...
Bach2 is a B cell-specific transcription repressor whose deficiency in mice causes reduced class switch recombination and somatic hypermutation of immunoglobulin genes. Little known about the direct target genes cells. By analyzing various cell plasma lines, we showed that expression patterns Blimp-1 (B lymphocyte-induced maturation protein 1), master regulator differentiation, are mutually exclusive. The reporter gene (Prdm1) was repressed by overexpression lines. heterodimer Bach2/MafK...
B lymphocyte-induced maturation protein 1 (Blimp-1) is a key regulator for plasma cell differentiation. Prior to the terminal differentiation into cells, Blimp-1 expression suppressed in cells by transcription repressors BTB and CNC homology 2 (Bach2) lymphoma 6 (Bcl6). Bach2 binds Maf recognition element (MARE) of promoter upstream region gene (Prdm1) forming heterodimer with MafK. Bcl6 were found interact each other cells. While both possess domain which mediates protein-protein...
nipbl-deficient zebrafish provide evidence that heart and gut defects in Cornelia de Lange Syndrome are caused by combined effects of multiple gene expression changes occur during early embryonic development.
Haploinsufficiency for Nipbl, a cohesin loading protein, causes Cornelia de Lange Syndrome (CdLS), the most common “cohesinopathy”. It has been proposed that effects of Nipbl-haploinsufficiency result from disruption long-range communication between DNA elements. Here we use zebrafish and mouse models CdLS to examine how transcriptional changes caused by Nipbl deficiency give rise limb defects, condition in individuals with CdLS. In pectoral fin (forelimb), knockdown expression led size...
Bach2 is a B-cell- and neuron-specific transcription repressor that forms heterodimers with the Maf-related oncoproteins. We show here activates by interacting its novel partner MAZR. MAZR was isolated yeast two-hybrid screen using BTB/POZ domain of as bait. Besides domain, possesses Zn finger motifs are closely related to those Myc-associated (MAZ) protein. mRNA coexpressed in B cells among hematopoietic developing mouse limb buds, suggesting cooperative role for these cells. homo-...
BTB and CNC homolog 1 (Bach1) is a transcriptional repressor of heme oxygenase-1 (HO-1), which plays an important role in the protection cells tissues against acute chronic inflammation. However, Bach1 gastrointestinal mucosal defense system remains little understood. HO-1 supports suppression experimental colitis localizes mainly macrophages colonic mucosa. This study was undertaken to elucidate Bach1/HO-1 system's effects on pathogenesis colitis. used C57BL/6 (wild-type) homozygous...