A5024749444- Histone Deacetylase Inhibitors Research
- Animal Nutrition and Physiology
- RNA Research and Splicing
- Ubiquitin and proteasome pathways
- Peptidase Inhibition and Analysis
- RNA and protein synthesis mechanisms
- Protein Degradation and Inhibitors
- ATP Synthase and ATPases Research
- Fungal and yeast genetics research
- T-cell and Retrovirus Studies
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Vector-Borne Animal Diseases
- Animal Disease Management and Epidemiology
- Microbial Natural Products and Biosynthesis
- Mitochondrial Function and Pathology
- Photonic Crystal and Fiber Optics
- Antifungal resistance and susceptibility
- Genomics and Chromatin Dynamics
- Chemical Synthesis and Analysis
- Cancer-related gene regulation
- Enzyme Structure and Function
- Meat and Animal Product Quality
- Click Chemistry and Applications
- Computational Drug Discovery Methods
RIKEN Center for Sustainable Resource Science
2016-2025
The University of Tokyo
2016-2025
RIKEN
2013-2024
Kindai University
2015-2024
Bunkyo University
1998-2024
Tokushima University
2015-2024
Japanese Red Cross Kumamoto Hospital
2009-2024
Takasaki University of Health and Welfare
2018-2024
Pioneer (Japan)
2024
Genomics (United Kingdom)
2012-2023
(R)-Trichostatin A (TSA) is a Streptomyces product which causes the induction of Friend cell differentiation and specific inhibition cycle normal rat fibroblasts in G1 G2 phases at very low concentrations. We found that TSA caused an accumulation acetylated histone species variety mammalian lines. Pulse-labeling experiments indicated markedly prolonged vivo half-life labile acetyl groups on histones mouse mammary gland tumor cells, FM3A. The partially purified deacetylase from wild-type FM3A...
The cellular target of leptomycin B (LMB), a nuclear export inhibitor, has been identified as CRM1 (exportin 1), an evolutionarily conserved receptor for the signal proteins. However, mechanism by which LMB inhibits still remains unclear. in Schizosaccharomyces pombe mutant showing extremely high resistance to had single amino acid replacement at Cys-529 with Ser. gene, named crm1-K1, conferred on wild-type S. , and Crm1-K1 no longer bound biotinylated LMB. 1 H NMR analysis showed that N...
The genome of human T-cell leukemia virus (HTLV) was surveyed in fresh tumor cells 163 patients with lymphoma and from the southwest part Japan where adult (ATL) is endemic. Leukemic all 88 cases ATL tested so far were found to contain provirus also be monoclonal respect integration site genome. In most ATL, leukemic contained one or two copies complete HTLV genome, it shown that single species a fully determined sequence typical ATL. Some malignancies, diagnosed as chronic lymphocytic...
High-pressure in situ x-ray-diffraction studies on GaN and InN have been carried out using an imaging-plate technique a diamond-anvil cell up to about 60 GPa. The two compounds crystallize the wurtzite-type structure at ambient conditions. axial ratio c/a of remains unchanged from value 1.626 whereas is considerably decreased 1.613 1.597 with increasing pressure 15 Equation-of-state data obtained for wurtzite phase yielded bulk modulus be 237(31) GPa that 125.5(4.6) Structural transition...
We developed a novel promoter system, designated SR alpha, which is composed of the simian virus 40 (SV40) early and R segment part U5 sequence (R-U5') long terminal repeat human T-cell leukemia type 1. The R-U5' stimulated chloramphenicol acetyltransferase (CAT) gene expression only when placed immediately downstream SV40 in sense orientation. alpha system was 1 or 2 orders magnitude more active than wide variety cell types, including fibroblasts lymphoid cells, capable promoting high level...
Anacardic acid attenuates mutant TDP-43–associated abnormalities in motor neurons derived from ALS patient–specific induced pluripotent stem cells.
Trapoxin (cyclo-(L-phenylalanyl-L-phenylalanyl-D-pipecolinyl-L-2-amino-8- oxo-9,10-epoxy-decanoyl)) is a fungal product that induces morphological reversion from transformed to normal in sis-transformed NIH3T3 fibroblasts. was found cause accumulation of highly acetylated core histones variety mammalian cell lines. In vitro experiments using partially purified mouse histone deacetylase showed low concentration trapoxin irreversibly inhibited deacetylation molecules. Chemical reduction an...
The molecular action of leptomycin B (LMB), an agent inducing arrest the eukaryotic cell cycle at G1 and G2 phases, was investigated by analyzing LMB resistance gene Schizosaccharomyces pombe. A genomic library LMB-resistant mutant screened for resistance, a DNA fragment containing open reading frame (ORF) 1078 amino acids cloned on multicopy vector. plasmid found to confer drug specifically LMB. Nucleotide sequencing revealed that ORF essential nuclear protein crm1, which had been reported...
Trichostatin A (TSA) and trapoxin (TPX) are potent inhibitors of histone deacetylases (HDACs). TSA is proposed to block the catalytic reaction by chelating a zinc ion in active-site pocket through its hydroxamic acid group. On other hand, epoxyketone suggested be functional group TPX capable alkylating enzyme. We synthesized novel analogue containing instead epoxyketone. The hybrid compound cyclic acid-containing peptide (CHAP) 1 inhibited HDAC1 at low nanomolar concentrations. inhibition...
Abstract We recently reported that circular RNA is efficiently translated by a rolling circle amplification (RCA) mechanism in cell-free Escherichia coli translation system. Recent studies have shown RNAs composed of exonic sequences are abundant human cells. However, whether these can be into proteins within cells remains unclear. In this study, we prepared with an infinite open reading frame and tested their eukaryotic systems. Circular were long rabbit reticulocyte lysate the absence any...