A5000488419- Fungal and yeast genetics research
- Radiation Effects and Dosimetry
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- Protein Degradation and Inhibitors
- Histone Deacetylase Inhibitors Research
- Radiation Therapy and Dosimetry
- Ubiquitin and proteasome pathways
- Bioinformatics and Genomic Networks
- Genomics and Chromatin Dynamics
- Microbial Metabolic Engineering and Bioproduction
- Genomics and Phylogenetic Studies
- Metal complexes synthesis and properties
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Radioactive element chemistry and processing
- Biofuel production and bioconversion
- Wastewater Treatment and Nitrogen Removal
- Advanced Proteomics Techniques and Applications
- Probiotics and Fermented Foods
- Dye analysis and toxicity
- Chemical Synthesis and Analysis
- Enzyme Structure and Function
- Nuclear Physics and Applications
- Listeria monocytogenes in Food Safety
RIKEN Center for Sustainable Resource Science
2014-2025
The University of Tokyo
1964-2025
RIKEN
1977-2024
Genomics (United Kingdom)
2016-2023
RIKEN Advanced Science Institute
2008-2013
Japan Science and Technology Agency
2002-2013
University at Buffalo, State University of New York
2006
Tokyo University of Science
2000
Tokyo University of Agriculture
1995
National Taiwan Normal University
1985
Elongator is required for the synthesis of mcm(5)s(2) modification found on tRNAs recognizing AA-ending codons. In order to obtain a global picture role in translation, we used reverse protein arrays screen fission yeast proteome translation defects. Unexpectedly, this revealed that inactivation mainly affected three specific functional groups including proteins implicated cell division. The absence results delay mitosis onset and cytokinesis We demonstrate kinase Cdr2, which central...
A novel series of plasmid vectors named pDUAL have been developed. These enable one to introduce not only multicopies genes with episomal maintenance but also a single copy chromosomal integration into the fission yeast, Schizosaccharomyces pombe. The multicopy plasmids can be easily converted fragments for by digestion certain restriction endonuclease before transformation yeast cells. resultant fragments, lacking autonomously replicating sequence, are designed targeting leu1 locus...
SDS-PAGE is a basic method that has long been used for separation of proteins according to their molecular sizes. Despite its simplicity, it provides information on characteristics beyond masses because gel mobility often reflects physicochemical properties and post-translational modifications. Here we report global analysis the proteome, which term "mobilitome," covering 93.4% fission yeast proteome. To our surprise, more than 40% did not migrate calculated positions. Statistical analyses...
Telomeres protect DNA ends of linear eukaryotic chromosomes from degradation and fusion, ensure complete replication the terminal through recruitment telomerase. The regulation telomerase is a critical area telomere research includes cis by shelterin complex in mammals fission yeast. We have identified key component this regulatory pathway as SUMOylation [the covalent attachment small ubiquitin-like modifier (SUMO) to target proteins] subunit known be involved negative extension telomerase;...
To establish a gene expression system that reflects physiological conditions, we developed series of vectors can be integrated into the chromosome. Compared with integration employing double-crossover recombination, single-crossover have advantage high transformation efficiency. However, because recombination generates repeat sequences upstream and downstream fragment, this strategy is often associated risk an fragment may pop out from chromosome during cultivation. Here, assessed frequency...
The fission yeast Schizosaccharomyces pombe has more metazoan-like features than the budding Saccharomyces cerevisiae, yet it similarly facile genetics. We present a large-scale verified binary protein-protein interactome network, "StressNet," based on high-throughput two-hybrid screens of interacting proteins classified as part stress response and signal transduction pathways in S. pombe. performed systematic, cross-species mapping using StressNet protein network orthologous cerevisiae....
Abstract Background Post-translational modifications (PTMs) have a key role in regulating cell functions. Consequently, identification of PTM sites has significant impact on understanding protein function and revealing cellular signal transductions. Especially, phosphorylation is ubiquitous process with large portion proteins undergoing this modification. Experimental methods to identify are labor-intensive high-cost. With the exponentially growing sequence data, development computational...
S ummary : Five strains of bacteria were irradiated, suspended in heart infusion broth or phosphate buffer, aerated anoxic conditions, at temperatures 10–13° ‐79°. Survivors under the different conditions enumerated by plate counts on agar. Exponential survivor‐dose curves obtained with a Pseudomonas strain and Escherichia coli B/r when irradiated room temperature aeration, whereas an Alcaligenes 2 Streptococcus faecium gave sigmoid curves. The decreased radiosensitivity frozen state was...
The efficient application of nitrogenous fertilizers is urgently required, as their excessive and inefficient use causing substantial economic loss environmental pollution. A significant amount applied nitrogen in agricultural soils lost nitrous oxide (N2O) the environment due to microbial denitrification process. widely distributed fungus Fusarium oxysporum a major denitrifier its activity could be targeted reduce form N2O from soils. Here, we report discovery first small molecule...
Abstract Microbes have evolved intricate communication systems that enable individual cells of a population to send and receive signals in response changes their immediate environment. In the fission yeast Schizosaccharomyces pombe , oxylipin nitrogen signaling factor (NSF) is part such system, which functions regulate usage different sources. Yet, pathways mechanisms by NSF acts are poorly understood. Here, we show physically interacts with mitochondrial sulfide:quinone oxidoreductase Hmt2...
Inducible/repressible promoters are useful for the maintenance of toxic genes or timely expression. For ectopic expression cloned in fission yeast Schizosaccharomyces pombe, thiamine-regulatable nmt1 promoter has been widely used, since transcriptional activity this can be controlled by thiamine. However, property sometimes limits a certain type research, inevitably requires cells to cultivated under conditions that induce activation. To allow constitutive heterologous genes, we three cam1+,...
Identification of the cellular target small molecules is a major challenge to developing biological tools and drug leads. Here we report novel microarray-based system for identification or pathway using set drug-hypersensitive fission yeast strains that collectively overexpress each gene in open reading frame-ome. The advantage this method it provides genome-wide interrogation but requires relatively amount test compound. Using system, identified 28 genes linked etoposide sensitivity, which...
Abstract The fission yeast ste7 mutant cannot mate and undergo meiosis, but shows no defect in vegetative growth. We cloned characterized the gene. deduced gene product (Ste7p) was a protein of 569 amino acids with significant similarity to other proteins. Transcription induced by nutrient starvation via function transcription factor Ste11p. Disruption blocked both conjugation showing that Ste7p plays positive role these two processes, probably activating pheromone signal pathway....