A5050723116- Microtubule and mitosis dynamics
- Ubiquitin and proteasome pathways
- Fungal and yeast genetics research
- Photosynthetic Processes and Mechanisms
- Cancer-related Molecular Pathways
- DNA Repair Mechanisms
- Genetics, Bioinformatics, and Biomedical Research
- Genomics and Chromatin Dynamics
- 14-3-3 protein interactions
- Epigenetics and DNA Methylation
- Mitochondrial Function and Pathology
- Bioinformatics and Genomic Networks
- Cellular transport and secretion
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Glycosylation and Glycoproteins Research
- ATP Synthase and ATPases Research
- RNA modifications and cancer
- Genetics and Neurodevelopmental Disorders
- Chromosomal and Genetic Variations
- Sperm and Testicular Function
- Pancreatic function and diabetes
- RNA and protein synthesis mechanisms
- PARP inhibition in cancer therapy
- CRISPR and Genetic Engineering
University College London
2015-2024
CRUK Lung Cancer Centre of Excellence
2015-2024
Osaka University
2023
Cancer Institute (WIA)
2019
Service Hydrographique et Océanographique de la Marine
2017
London Cancer
2012-2016
The University of Tokyo
2016
Kyushu University
2013-2014
Marie Curie
2004-2011
Kyoto University
1991-2007
SpCas9 and AsCas12a are widely utilized as genome-editing tools in human cells. However, their relatively large size poses a limitation for delivery by cargo-size-limited adeno-associated virus (AAV) vectors. The type V-F Cas12f from Acidibacillus sulfuroxidans is exceptionally compact (422 amino acids) has been harnessed tool. Here, we developed an approach, combining deep mutational scanning structure-informed design, to successfully generate two AsCas12f activity-enhanced (enAsCas12f)...
Phosphorylation cues exit from mitosis The entry and the cell cycle are controlled by waves of protein phosphorylation degradation events. Fujimitsu et al. describe precise mechanism which machinery controls mitosis. critical event is activation a ubiquitin ligase, anaphase-promoting complex or cyclosome (APC/C). authors used purified components Xenopus egg extract system to show that two subunits APC/C were directly phosphorylated cyclin-dependent kinase 1 (CDK1). one subunit helped recruit...
<ns4:p>The separation of sister chromatids at anaphase, which is regulated by an E3 ubiquitin ligase called the anaphase-promoting complex/cyclosome (APC/C), arguably most important irrevocable event during cell cycle. The APC/C and cyclin-dependent kinase 1 (Cdk1) are just two many significant cycle regulators exert control through ubiquitylation phosphorylation, respectively. temporal spatial regulation achieved multiple mechanisms, including interaction with structurally related...
To understand the role of type 2A-like protein phosphatase in cell division cycle, we investigated mutant phenotypes obtained when fission yeast ppa1+ and ppa2+ genes (which encode polypeptides with approximately 80% identity to mammalian 2A phosphatases) were either deleted or overexpressed. We also vivo effect okadaic acid, an inhibitor serine/threonine phosphatases, on division. show that interacts genetically regulators cdc25+ wee1+, as a ppa2 deletion is lethal combined wee1-50 but...
Cell-cycle progression and cell division in eukaryotes are governed part by the cyclin family their regulation of cyclin-dependent kinases (CDKs). Cyclins very well characterised model systems such as yeast human cells, but surprisingly little is known about number role Plasmodium, unicellular protozoan parasite that causes malaria. Malaria proliferation differs from many eukaryotes. During its life cycle it undergoes two types mitosis: endomitosis asexual stages an extremely rapid mitotic...
The fission yeast pap1+ gene encodes an AP-1-like transcription factor that contains a leucine zipper motif. We identified target of pap1, the p25 gene. 5' upstream region AP-1 site, and by DNase I footprint analysis, we showed pap1 protein binds to site as well 14-bp palindrome sequence. is overproduced when overexpressed, whereas not produced at all in deletion mutant. was previously found be strains carrying cold-sensitive crm1 mutations whose product essential for viability thought play...
The Anaphase-promoting complex/cyclosome (APC/C) cofactor Cdh1 modulates cell proliferation by targeting multiple cell-cycle regulators for ubiquitin-dependent degradation. Lack of results in structural and numerical chromosome aberrations, a hallmark genomic instability. By using proteomic approach Cdh1-null cells mouse tissues, we have identified kinesin Eg5 topoisomerase 2α as targets involved the maintenance stability. These proteins are ubiquitinated degraded through specific KEN D...
The anaphase promoting complex/cyclosome (APC/C) is a highly conserved multi-subunit E3 ubiquitin ligase that controls mitotic division in eukaryotic cells by tagging cell cycle regulators for proteolysis. APC3 key component contributes to APC/C function. Plasmodium, the causative agent of malaria, undergoes atypical during its life cycle. Only small subset components has been identified Plasmodium and their involvement not well understood. Here, using reverse genetics we examined...
Abstract A multi-subunit ubiquitin ligase, the anaphase-promoting complex/cyclosome (APC/C), regulates critical cellular processes including cell cycle. To accomplish its diverse functions, APC/C activity must be precisely regulated in time and space. The interphase activator Fizzy-related (Fzr or Cdh1) is localized at centrosomes animal cells. However, neither mechanism of localization nor importance clear. Here we identify centrosome component Spd2 as a major partner Fzr Drosophila. to...