A5086107328- Virus-based gene therapy research
- Cancer-related Molecular Pathways
- Viral Infectious Diseases and Gene Expression in Insects
- RNA Interference and Gene Delivery
- DNA Repair Mechanisms
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Viral gastroenteritis research and epidemiology
- Cardiomyopathy and Myosin Studies
- Cancer Research and Treatments
- RNA Research and Splicing
- Cell death mechanisms and regulation
- Kruppel-like factors research
- Cytomegalovirus and herpesvirus research
- Cancer-related gene regulation
- interferon and immune responses
- CAR-T cell therapy research
- RNA modifications and cancer
- Meat and Animal Product Quality
- Viral-associated cancers and disorders
- Viral Infections and Immunology Research
- Herpesvirus Infections and Treatments
- Ubiquitin and proteasome pathways
- Ion channel regulation and function
- Biotin and Related Studies
University of Birmingham
2015-2025
Cancer Research UK
1987-2012
Cancer Research UK Clinical Trials Unit
1984-2010
Western University
2009
Princeton University
1995
University of Dundee
1993
University of Oxford
1982
Howard Hughes Medical Institute
1975-1976
University of Miami
1975-1976
Royal Holloway University of London
1975-1976
1. A series of defined peptides which span the complete sequence were produced from troponin I isolated white skeletal muscle rabbit. 2. Two peptides, CF1 (residues 64-133) and CN4 96-117) inhibited Mg2+-stimulated adenosine triphosphatase desensitized actomyosin. This inhibition was potentiated by tropomyosin inhibition, unlike that derived it, not tropomyosin. 4. The most active inhibitor, peptide CN4, 45-75% as effective when compared on a molar basis. inhibitory peptide, also whole shown...
1. An acidic protein with properties similar to those of troponin C from rabbit skeletal muscle has been shown be present in bovine and smooth muscles, chicken gizzard liver, kidney lung. 2. A simple new method involving the use organic solvents is described for purification C-like proteins various tissues. 3. The can distinguished skeletal-muscle toponin by their electrophoretic behaviour on polyacrylamide gels at pH 8.3 presence absence Ca2+. have form complexes I that migrate...
The amino acid sequence of the phosphorylase kinase δ subunit has been determined using cyanogen bromide peptides. These peptides were ordered by isolation tryptic containing carboxy[ 14 C]methylmethionine. protein is identical to bovine uterus calmodulin and differs from brain only in amide assignments. contains 148 acids, including one residue trimethyllysine, a molecular weight 16680. N terminus blocked, probably with an acetyl group, net overall charge at pH 7 –24. asparagine residues 60...
The calmodulin contents of rabbit brain, lung, kidney and liver, bovine aorta uterus, chicken gizzard have been determined. 2. in all these tissues has shown to be present the form very stable complexes with several other proteins. 3. A calmodulin-binding protein mol.wt. 22 000 purified high yield from brain. It interact skeletal-muscle troponin C a Ca2+-dependent manner. 4. 000-mol.wt. inhibits activation brain phosphodiesterase by calmodulin, but little affect on myosin light-chain kinase....
The complete amino acid sequence of rabbit skeletal muscle troponin I was determined by the isolation cyanogen bromide fragments and tryptic methionine-containing peptides. Troponin contains 179 residues has a molecular weight 20864. Its N-terminus is acetylated. Detailed evidence on which based been deposited as Supplementary Publication SUP 50055 (23 pages) at British Library (Lending Division), Boston Spa, Wetherby, West Yorkshire LS23 7QB, U.K., from whom copies may be obtained terms...
ABSTRACT Recent reports suggest that an early region 1B (E1B) 55,000-molecular-weight polypeptide (55K)-null adenovirus type 5 (Ad5) mutant ( dl 1520) can replicate to the same extent as wild-type (wt) Ad5 in cells either deficient or mutated p53, implicating p53 limiting viral replication vivo. In contrast, we show here replicative capacity of 1520 is wholly independent host cell status, comparable Ad12 E1B 54K-null adenoviruses (Ad12 620 and hr 703). Furthermore, there no requirement for...
The complete amino acid sequence of troponin I from rabbit cardiac muscle was determined by the isolation four unique CNBr fragments, together with overlapping tryptic peptides containing radioactive methionine residues. Overlap data for residues 35-36, 93-94 and 140-145 are incomplete, at these positions being based on homology fast-skeletal-muscle protein. Cardiac is a single polypeptide chain 206 mol.wt. 23550 an extinction coefficient, E 1%,1cm/280, 4.37. protein has net positive charge...
Activation of the cellular DNA damage response is detrimental to adenovirus (Ad) infection. Ad has therefore evolved a number strategies inhibit ATM- and ATR-dependent signaling pathways during Recent work suggests that Ad5 E4orf3 protein prevents ATR activation through its ability mislocalize MRN complex. Here we provide evidence indicate Ad12 different strategy from ATR. We show utilizes CUL2/RBX1/elongin C-containing ubiquitin ligase promote proteasomal degradation activator...
1. Calmodulin-like proteins were purified from the fruiting bodies of higher (basidiomycete) fungi and barley (Hordeum sp.) shoots. 2. These calmodulins have electrophoretic mobilities on 10% (w/v) polyacrylamide gels at pH 8.3 in presence 6 M-urea 0.1% sodium dodecyl sulphate similar to that bovine brain calmodulin. They interacted with rabbit skeletal-muscle troponin I Ca2+. 3. Barley fungal activated myosin light-chain kinase phosphodiesterase Ca2+, although amounts needed least an order...
1. A calmodulin-binding protein of apparent mol.wt. 19 000 has been purified from chicken gizzard. Similar proteins have isolated bovine uterus, rabbit skeletal muscle and liver. 2. These migrated as an equimolar complex with brain calmodulin on electroporesis polyacrylamide gels in the presence Ca2+ 6M-urea. The was dissociated EGTA. gizzard shown to be identical erythrocyte histone H2B basis partial amino acid sequence determination. 4. 22 previously [Grand & Perry (1979) Biochem. J....