A5010162332- T-cell and B-cell Immunology
- Alzheimer's disease research and treatments
- Advancements in Photolithography Techniques
- SARS-CoV-2 and COVID-19 Research
- Nanofabrication and Lithography Techniques
- Dyeing and Modifying Textile Fibers
- Immune Cell Function and Interaction
- Spaceflight effects on biology
- Virus-based gene therapy research
- Cytomegalovirus and herpesvirus research
- Parkinson's Disease Mechanisms and Treatments
- COVID-19 Clinical Research Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Crustacean biology and ecology
- Semiconductor materials and devices
- Tryptophan and brain disorders
- SARS-CoV-2 detection and testing
- Ion-surface interactions and analysis
- CRISPR and Genetic Engineering
- Silk-based biomaterials and applications
- Cell Image Analysis Techniques
- Marine Biology and Ecology Research
- Hair Growth and Disorders
- Radiation Therapy and Dosimetry
- Dementia and Cognitive Impairment Research
Ehime University
2015-2025
Kyoto University
2019-2024
Osaka University
2014-2023
Tokai University
2011-2020
Matsumoto Dental University
2019-2020
Tohoku University
2018-2020
Osaka Prefecture University
2013-2016
University of Tsukuba
2011-2012
Nara Women's University
2006-2011
NTT (Japan)
1982-1983
SpCas9 and AsCas12a are widely utilized as genome-editing tools in human cells. However, their relatively large size poses a limitation for delivery by cargo-size-limited adeno-associated virus (AAV) vectors. The type V-F Cas12f from Acidibacillus sulfuroxidans is exceptionally compact (422 amino acids) has been harnessed tool. Here, we developed an approach, combining deep mutational scanning structure-informed design, to successfully generate two AsCas12f activity-enhanced (enAsCas12f)...
The cerebrum is a major center for brain function, and its activity derived from the assembly of activated cells in neural networks. It currently difficult to study complex human cerebral neuronal network activity. Here, using organoids, we report self-organized activities that include synchronized non-synchronized patterns. Self-organized formation was observed following dissociation culture embryonic stem cell-derived organoids. spontaneous individual measured via calcium imaging,...
The Omicron (B.1.1.529) SARS-CoV-2 variant contains an unusually high number of mutations in the spike protein, raising concerns escape from vaccines, convalescent serum, and therapeutic drugs. Here, we analyzed degree to which pseudo-virus evades neutralization by serum or antibodies. Serum samples obtained 3 months after two doses BNT162b2 vaccination exhibited 18-fold lower titers against than parental virus. Convalescent individuals infected with Alpha Delta variants allowed similar...
How and why diverse sexual systems evolve are fascinating evolutionary questions, but few empirical studies have dealt with these questions in animals. Pedunculate (gooseneck) barnacles show such diversity, including simultaneous hermaphroditism, coexistence of dwarf males hermaphrodites (androdioecy), females (dioecy). Here, we report the first phylogenetically controlled test hypothesis that ultimate cause presence this group is limited mating opportunities for non-dwarf individuals, owing...
Cyclin G-associated kinase (GAK), a key player in clathrin-mediated membrane trafficking, is overexpressed various cancer cells. Here, we report that GAK expression positively correlated with the Gleason score surgical specimens from prostate patients. Embryonic fibroblasts knockout mice expressing kinase-dead (KD) form of showed constitutive hyper-phosphorylation epidermal growth factor receptor (EGFR). In addition to well-known EGFR inhibitors gefitinib and erlotinib, dietary flavonoid...
Withaferin A (WA), a major bioactive component of the Indian herb Withania somnifera, induces cell death (apoptosis/necrosis) in multiple types tumor cells, but molecular mechanism underlying this cytotoxicity remains elusive. We report here that 2 μM WA induced selectively androgen-insensitive PC-3 and DU-145 prostate adenocarcinoma whereas its toxicity was less severe androgen-sensitive LNCaP cells normal human fibroblasts (TIG-1 KD). also killed spheroid-forming medium. DNA microarray...
Intracerebral hemorrhage is reported to induce the generation of reactive oxygen species and oxidative DNA damage in brain.We aimed examine whether our designed redox polymer nanoparticle could reduce intracerebral induced by 1-MHz focused ultrasound sonication coupled with microbubble treatment.Contrast-enhanced imaging, frozen section, brain edema, neurologic deficit, number morphologically normal neurons, oxidization superoxide anion were used investigate neuroprotective effect...
Adaptive immunity is a fundamental component in controlling COVID-19. In this process, follicular helper T (Tfh) cells are subset of CD4+ that mediate the production protective antibodies; however, SARS-CoV-2 epitopes activating Tfh not well characterized. Here, we identified and crystallized TCRs public circulating (cTfh) clonotypes expanded patients who have recovered from mild symptoms. These recognized spike (S) conserved across emerging variants. The epitope most prevalent cTfh...
ABSTRACT Background Maternal psychiatric condition during the perinatal period is relevant to children's cognitive development and mental health. Psychotropic medications are necessary maintain health of pregnant women with disorders, but they often avoided due concerns about adverse effects, such as congenital malformations abnormal neurodevelopment. A retrospective study disorders using psychotropic was performed clarify maternal child demographic data investigate whether affected Apgar...
Despite the continuing debate about amyloid hypothesis in Alzheimer's disease (AD), precise pathogenesis is still unclear. Mixed pathology common and multiple different protein aggregates are seen human postmortem brains. Aggregates consisting of alpha-synuclein encoded by Synuclein Alpha gene (SCNA) both dementia with Lewy bodies AD. We examined SNCA mRNA expression methylation rates CpG island at intron 1 peripheral leukocytes 50 AD age- sex-matched control subjects to verify whether...
TREM2 and TYROBP are causal genes for Nasu–Hakola disease (NHD), a rare autosomal recessive characterized by bone lesions early-onset progressive dementia. forms receptor signaling complex with TYROBP, which triggers the activation of immune responses in macrophages dendritic cells, functional polymorphism is reported to be associated neurodegenerative disorders such as Alzheimer's (AD). The objective this study was reveal involvement pathophysiology AD schizophrenia. Methods: We...
Background: We explored the gene expression levels in brain of 3xTg-AD model mice to elucidate molecular pathological changes from early end stages Alzheimer’s disease (AD). Objective: re-analyzed our previously published microarray data obtained hippocampus at 12 and 52 weeks age. Methods: Functional annotation network analyses up- downregulated differentially expressed genes (DEGs) aged were performed. Validation tests for gamma-aminobutyric acid (GABA)-related also performed by...
Abstract The testing of pathological biomarkers Alzheimer’s disease (AD), such as amyloid beta and tau, is time-consuming, expensive, invasive. Here, we used 3xTg-AD mice to identify validate putative novel blood transcriptome AD that can potentially be identified in the patients. mRNA was extracted from hippocampus control at different ages for microarray analysis. Network functional analyses revealed differentially expressed genes between modulated immune neuroinflammation systems. Five...
Organoids, self-organized cell aggregates, contribute significantly to developing disease models and cell-based therapies. Organoid-to-organoid variations, however, are inevitable despite the use of latest differentiation protocols. Here, we focused on morphology organoids formed in a cerebral organoid culture assessed their cellular compositions by single-cell RNA sequencing analysis. The data revealed that primarily composed non-neuronal cells, such as those from neural crest choroid...
The sexuality of large (non-dwarf) individuals and the characteristics eggs, larvae dwarf males were investigated in pedunculate barnacle Scalpellum stearnsii . All 103 collected this study not hermaphrodites but females. major length axis egg was on average 0.50 mm, which larger than that most thoracican barnacles with planktotrophic larvae. hatched as nauplii metamorphosed into cyprids without feeding (i.e. lecithotrophic development). There a positive relationship between body weight mass...
A hypothesis for schizophrenia (SCZ) called the "microglia hypothesis" has been suggested. In SCZ, expression of triggering receptor expressed on myeloid cell 2 (TREM2) mRNA is higher in leukocytes than healthy individuals. Here, methylation rates four CpG sites TREM2 intron 1 that may bind important transcription factors and correlation between rate were determined. We compared SCZ patients age-matched controls (n = 50 each). had significantly lower (17.0 ± 6.7 vs. 20.2 5.0; p 0.02) 3 (23.8...
Abstract The novel SARS-CoV-2 variant, Omicron (B.1.1.529) contains an unusually high number of mutations (>30) in the spike protein, raising concerns escape from vaccines, convalescent sera and therapeutic drugs. Here we analyze alteration neutralizing titer with pseudovirus. Sera obtained 3 months after double BNT162b2 vaccination exhibit approximately 18-fold lower neutralization titers against than parental virus. Convalescent Alpha Delta patients allow similar levels breakthrough by...
By scanning transmission electron microscopy and energy dispersive X-ray spectroscopy (STEM–EDS), we investigated the elemental depth profiles of organic beam resist films after sequential infiltration synthesis (SIS) inorganic alumina. Although a 40-nm-thick poly(methyl methacrylate) (PMMA) film was entirely hybridized with alumina, an uneven distribution observed near interface between substrate as well surface. The around center 100-nm-thick PMMA film. thicknesses CSAR62 decreased almost...
The clinical features in cases that have mutations the microtubule‐associated protein tau gene but lack prominent behavioral changes remain unclear. Here, we describe detailed and pathological of a case carrying P301L mutation showed only apathy until middle stage course. mother this was suspected to mild cognitive decline at age 46. However, before she fully examined, had subarachnoid hemorrhage 49 died 53. An autopsy not done. proband pedigree, 60‐year‐old right‐handed Japanese man time...
Dry mouth, caused by decreased salivary gland function and/or weak stimulation, can severely affect oral health in older individuals. Therefore, the aim of this study is to evaluate whether a lip trainer device and sonic toothbrush improve flow wetness patients complaining dry mouth. Overall, 39 subjects aged ≥60 years who had at least 20 natural teeth were randomly assigned use (group P, n = 13) or S, 13). The did not experience dryness included as controls C; unstimulated stimulated rates...
Abstract It is very rare that cases of Pick's disease, a representative three‐repeat (3R) tauopathy, also have significant four‐repeat (4R) tau accumulation. Here, we report disease case clinically showed behavioral variant frontotemporal dementia without motor disturbance during the course, and pathologically had 3R tau‐positive Pick bodies as well numerous 4R neuronal cytoplasmic inclusions (NCIs). Abundant tau‐negative spherical or horseshoe‐shaped were found in cortex, limbic region,...