A5083675154- RNA Research and Splicing
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Bioinformatics and Genomic Networks
- Fungal and yeast genetics research
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Cell Adhesion Molecules Research
- RNA Interference and Gene Delivery
- Advanced Proteomics Techniques and Applications
- NF-κB Signaling Pathways
- Protein Structure and Dynamics
- Peptidase Inhibition and Analysis
- Genomics and Phylogenetic Studies
- Chromosomal and Genetic Variations
Michigan State University
2022-2025
Michigan United
2025
Cornell University
2013-2020
National Cancer Institute
2019
National Institutes of Health
2019
Pediatrics and Genetics
2015
Given the inherent difficulties in investigating mechanisms of tumor progression vivo, cell-based assays such as soft agar colony formation assay (hereafter called assay), which measures ability cells to proliferate semi-solid matrices, remain a hallmark cancer research. A key advantage this technique over conventional 2D monolayer or 3D spheroid cell culture is close mimicry cellular environment that seen vivo. Importantly, also provides an ideal tool rigorously test effects novel compounds...
Aging is a complex phenomenon that characterized by the altered regulation of various biological processes over time. One these, epigenetics, play crucial role throughout different stages eukaryotic life and its alteration considered key molecular hallmark aging. However, epigenetic factors which are important for lifespan control remain elusive. Here, we used S. pombe as model organism to study basis Our reveals loss epe1 + gene, encoding JmjC domain protein Epe1 , extends chronological...
The fission yeast Schizosaccharomyces pombe has more metazoan-like features than the budding Saccharomyces cerevisiae, yet it similarly facile genetics. We present a large-scale verified binary protein-protein interactome network, "StressNet," based on high-throughput two-hybrid screens of interacting proteins classified as part stress response and signal transduction pathways in S. pombe. performed systematic, cross-species mapping using StressNet protein network orthologous cerevisiae....
In eukaryotic genomes, heterochromatin is targeted by RNAi machinery and/or pathways requiring RNA elimination and transcription termination factors. However, a direct connection between polymerase II (RNAPII) transcriptional activity at heterochromatic loci has remained elusive. Here, we show that, in fission yeast, the conserved cleavage polyadenylation factor (CPF) key component involved RNAi-independent assembly of constitutive facultative domains that CPF broadly required to silence...
Gene regulatory mechanisms rely on a complex network of RNA processing factors to prevent untimely gene expression. In fission yeast, the highly conserved ortholog human ERH, called Erh1, interacts with YTH family binding protein Mmi1 form Erh1-Mmi1 (EMC) implicated in gametogenic silencing. However, structural basis EMC assembly and its functions are poorly understood. Here, we present co-crystal structure that consists Erh1 homodimers interacting 2:2 stoichiometry via molecular interface....
Significance Although recent advances in next-generation sequencing have facilitated the construction of whole genomes from hundreds organisms, considerable barriers still restrict functional understanding genes that they contain. An initial prerequisite for such an is availability high-quality gene libraries (ORFeomes) amenable to high-throughput experiments. Here we develop a massively parallel method, PLATE-seq, and leverage method construct ORFeome rice, providing toolkit systematic...
Given the inherent difficulties in investigating mechanisms of tumor progression vivo, cell-based assays such as soft agar colony formation assay (hereafter called assay), which measures ability cells to proliferate semi-solid matrices, remain a hallmark cancer research. A key advantage this technique over conventional 2D monolayer or 3D spheroid cell culture is close mimicry cellular environment that seen vivo. Importantly, also provides an ideal tool rigorously test effects novel compounds...
Abstract N 6 ‐methyladenosine (m6A) modifications of RNAs are associated with diverse biological processes in normal and cancer cells. In melanoma cancers, mutations genes that function the writing or erasure RNA m6A have been shown to affect progression metastasis. More recently, Shi et al. find a protein which binds modification, called YTHDF3, is widely overexpressed cell lines patient samples. Through multi‐omics targeted analyses, they uncover novel YTHDF3‐LOXL3 axis contribute vitro...