A5054138207- Algal biology and biofuel production
- Genomics and Phylogenetic Studies
- Microbial Metabolic Engineering and Bioproduction
- Bioinformatics and Genomic Networks
- RNA and protein synthesis mechanisms
- Microbial Community Ecology and Physiology
- RNA Research and Splicing
- Photosynthetic Processes and Mechanisms
- Marine and coastal ecosystems
- Protist diversity and phylogeny
- Saffron Plant Research Studies
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Biofuel production and bioconversion
- T-cell and Retrovirus Studies
- Marine and coastal plant biology
- Epigenetics and DNA Methylation
- Marine Biology and Ecology Research
- Molecular Biology Techniques and Applications
- Cellular transport and secretion
- Coastal wetland ecosystem dynamics
- Vector-Borne Animal Diseases
- Animal Disease Management and Epidemiology
- Flavonoids in Medical Research
- Biocrusts and Microbial Ecology
New York University
2014-2024
New York University Abu Dhabi
2014-2024
Harvard University
1997-2023
Dana-Farber Cancer Institute
2006-2020
Boston VA Research Institute
2020
United Arab Emirates University
2016
Universitat de Barcelona
2012
University of Lausanne
2012
Harvard University Press
2008
Howard Hughes Medical Institute
2001-2006
Three-prime untranslated regions (3'UTRs) of metazoan messenger RNAs (mRNAs) contain numerous regulatory elements, yet remain largely uncharacterized. Using polyA capture, 3' rapid amplification complementary DNA (cDNA) ends, full-length cDNAs, and RNA-seq, we defined approximately 26,000 distinct 3'UTRs in Caenorhabditis elegans for 85% the 18,328 experimentally supported protein-coding genes revised 40% gene models. Alternative 3'UTR isoforms are frequent, often differentially expressed...
The classic organization of a gene structure has followed the Jacob and Monod bacterial model proposed more than 50 years ago. Since then, empirical determinations complexity transcriptomes found in yeast to human blurred definition physical boundaries genes. Using multiple analysis approaches we have characterized individual mapping on chromosomes 21 22. Analyses locations 5′ 3′ transcriptional termini 492 protein coding genes revealed that for 85% these extend beyond current annotated...
Ribozymes are thought to have played a pivotal role in the early evolution of life, but relatively few been identified modern organisms. We performed an vitro selection aimed at isolating self-cleaving RNAs from human genome. The yielded several ribozymes, one which is conserved mammalian sequence that resides intron CPEB3 gene, belongs family genes regulating messenger RNA polyadenylation. ribozyme structurally and biochemically related hepatitis delta virus (HDV) ribozymes. occurrence this...
We are engaged in a long-term effort to synthesize chemical systems capable of Darwinian evolution, based on the encapsulation self-replicating nucleic acids membrane vesicles. Here, we address issue compatibility these two replicating systems. Fatty form vesicles that able grow and divide, but composed solely fatty incompatible with folding activity most ribozymes, because low concentrations divalent cations (e.g., Mg(2+)) cause precipitate. Furthermore, divide must be permeable substrates...
In eukaryotic cells the stability and function of many proteins are regulated by addition ubiquitin or ubiquitin-like peptides. This process is dependent upon sequential action an E1-activating enzyme, E2-conjugating E3 ligase. Different combinations these confer substrate specificity form protein modification. However, combinatorial preferences within ubiquitination networks remain unclear. this study, yeast two-hybrid (Y2H) screens were combined with true homology modeling methods to...
Increased risk for autism spectrum disorders (ASD) is attributed to hundreds of genetic loci. The convergence ASD variants have been investigated using various approaches, including protein interactions extracted from the published literature. However, these datasets are frequently incomplete, carry biases and limited a single splicing isoform, which may not be expressed in disease-relevant tissue. Here we introduce new interactome mapping approach by experimentally identifying between...
Since its start, the Mammalian Gene Collection (MGC) has sought to provide at least one full-protein-coding sequence cDNA clone for every human and mouse gene with a RefSeq transcript, 6200 rat genes. The MGC cloning effort initially relied on random expressed tag screening of libraries. Here, we summarize our recent progress using directed RT-PCR DNA synthesis. now contains clones entire protein-coding 92% 89% genes curated (NM-accession) transcripts, 97% 96% transcripts that have or more...
We performed whole-genome resequencing of 12 field isolates and eight commonly studied laboratory strains the model organism Chlamydomonas reinhardtii to characterize genomic diversity provide a resource for studies natural variation. Our data support previous observations that is among most diverse eukaryotic species. Nucleotide ∼3% geographically structured in North America with some evidence admixture sampling locales. Examination predicted loss-of-function mutations indicates...
Poor prognoses remain the most challenging aspect of hepatocellular carcinoma (HCC) therapy. Consequently, alternative therapeutics are essential to control HCC. This study investigated anticancer effects safranal against HCC using in vitro, silico, and network analyses. Cell cycle immunoblot analyses key regulators cell cycle, DNA damage repair apoptosis demonstrated unique safranal-mediated arrest at G2/M phase 6 12 h, S-phase 24 a pronounced effect on machinery. Safranal also showed...
The spice saffron (Crocus sativus) has anticancer activity in several human tissues, but the molecular mechanisms underlying its potential therapeutic effects are poorly understood. We investigated impact of safranal, a small molecule secondary metabolite from saffron, on HCC cell line HepG2 using untargeted metabolomics (HPLC–MS) and transcriptomics (RNAseq). Increases glutathione disulfide other biomarkers for oxidative damage contrasted with lower levels antioxidants biliverdin IX...
Macroalgae are multicellular, aquatic autotrophs that play vital roles in global climate maintenance and have diverse applications biotechnology eco-engineering, which directly linked to their multicellularity phenotypes. However, genomic diversity the evolutionary mechanisms underlying these organisms remain uncharacterized. In this study, we sequenced 110 macroalgal genomes from climates phyla, identified key features distinguish them microalgal relatives. Genes for cell adhesion,...
The angiogenesis inhibitor, sorafenib, remains the only available therapy of hepatocellular carcinoma (HCC). Only recently patents VEGF receptors-3 inhibitors are developed. Thus, a novel approach against HCC is essential for better therapeutic outcome.The aims this study were to examine chemopreventive action saffron's main biomolecule, crocin, chemically-induced liver cancer in rats, and explore mechanisms by which crocin employs its anti-tumor effects.We investigated anti-cancer effect on...