A5014704347- Bioinformatics and Genomic Networks
- Gene expression and cancer classification
- Cancer Genomics and Diagnostics
- Genomics and Chromatin Dynamics
- Genomic variations and chromosomal abnormalities
- Genetic Mapping and Diversity in Plants and Animals
- Molecular Biology Techniques and Applications
- RNA modifications and cancer
- Genetic Associations and Epidemiology
- Epigenetics and DNA Methylation
- Immunotherapy and Immune Responses
- Genomics and Phylogenetic Studies
- Genetics, Bioinformatics, and Biomedical Research
- Cleft Lip and Palate Research
- Craniofacial Disorders and Treatments
- Biomedical and Engineering Education
- RNA Research and Splicing
- Genetic factors in colorectal cancer
- Genetics and Neurodevelopmental Disorders
- Glutathione Transferases and Polymorphisms
- Biomedical Text Mining and Ontologies
- Liver Disease Diagnosis and Treatment
- Hormonal Regulation and Hypertension
- Cancer-related Molecular Pathways
- Chromosomal and Genetic Variations
Arizona State University
2014-2025
Mayo Clinic in Arizona
1999-2021
Tel Aviv University
2018
Bar-Ilan University
2018
TU Dortmund University
2018
National Institutes of Health
2004-2014
National Cancer Institute
2005-2014
Laboratory of Molecular Genetics
2012
GlaxoSmithKline (United States)
2011
Sage Bionetworks
2011
The National Institutes of Health Mammalian Gene Collection (MGC) Program is a multiinstitutional effort to identify and sequence cDNA clone containing complete ORF for each human mouse gene. ESTs were generated from libraries enriched full-length cDNAs analyzed candidate full-ORF clones, which then sequenced high accuracy. MGC has currently verified the full nonredundant set >9,000 >6,000 genes. Candidate clones an additional 7,800 3,500 genes also have been identified. All sequences...
The Pathway Interaction Database (PID, http://pid.nci.nih.gov) is a freely available collection of curated and peer-reviewed pathways composed human molecular signaling regulatory events key cellular processes. Created in collaboration between the US National Cancer Institute Nature Publishing Group, database serves as research tool for cancer community others interested pathways, such neuroscientists, developmental biologists immunologists. PID offers range search features to facilitate...
The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing complete open reading frame (ORF) for every human mouse gene. initially used random strategy select clones from large number libraries diverse tissues. Candidate were chosen based on 5′-EST sequences, then fully sequenced high accuracy analyzed by algorithms developed this project. Currently, more than 11,000 10,000 genes are...
In the last few years there have been rapid advances in developing genetic maps for humans, greatly enhancing our ability to localize and identify genes inherited disorders. Through collaborative efforts of three large groups generating microsatellite markers 110 CEPH collaborators, a comprehensive human linkage map is presented here. It consists 5840 loci, which 970 are uniquely ordered, covering 4000 centimorgans on sex-averaged map. Of these 3617 polymerase chain reaction-formatted short...
We describe here a system for the rapid identification, assay development, and characterization of gene-based single nucleotide polymorphisms (SNPs). This couples informatics tools that mine candidate SNPs from public expressed sequence tag resources automatically designs reagents with detection by chip-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry platform. As proof concept this system, genomewide collection 9,115 SNP genetic markers was rapidly...
Variations in gene sequence and expression underlie much of human variability. Despite the known biological roles differential allelic resulting from X-chromosome inactivation genomic imprinting, a large-scale analysis is lacking. We examined allele-specific 1063 transcribed single-nucleotide polymorphisms (SNPs) by using Affymetrix HuSNP oligo arrays. Among 602 genes that were heterozygous expressed kidney or liver tissues seven individuals, 326 (54%) showed preferential one allele at least...
Finding better therapies for the treatment of brain tumors is hampered by lack consistently obtained molecular data in a large sample set and ability to integrate biomedical from disparate sources enabling translation bench bedside. Hence, critical factor advancement research clinical ease with which can be integrated, redistributed, analyzed both within across functional domains. Novel informatics infrastructure tools are essential developing individualized patient based on specific genomic...
A gene's expression pattern provides clues to its role in normal physiology and disease. To provide quantitative levels on a genome-wide scale, the Cancer Genome Anatomy Project (CGAP) uses serial analysis of gene (SAGE). Over 5 million transcript tags from more than 100 human cell types have been assembled. enhance utility this data, CGAP SAGE project created Genie, web site for presentation data (http://cgap.nci.nih.gov/SAGE). Genie an automatic link between names levels, accounting...
Metastasis is one of the most important and complex processes in human neoplastic disease. A large number both positive negative events must occur to permit a tumor cell colonize distant site successfully. To identify mouse strains that harbor dominant genetic modifiers this process, strain survey was initiated utilizing transgenic mammary model exhibits high incidence pulmonary metastases. The animal bred 27 different inbred mice scored for metastatic organ tropism density. Thirteen were...
Asthma is a complex heritable inflammatory disorder of the airways associated with clinical signs atopy and bronchial hyperresponsiveness. Recent studies localized major gene for asthma to chromosome 5q31-q33 in humans. Thus, this segment genome represents candidate region genes that determine susceptibility hyperresponsiveness animal models. Homologs on human are found four regions mouse genome, two 18, one each chromosomes 11 13. We assessed responsiveness as quantitative trait mice it...
We immunohistochemically examined 12 core proteins involved in the chromatin remodeling machinery using a tissue microarray composed of 150 lung adenocarcinoma (AD) and squamous cell carcinoma (SCC) cases. Most showed nuclear staining, whereas some also cytoplasmic or membranous staining. When expression patterns all tested antigens were considered, with staining clustered into two major groups. Nuclear signals BRM, Ini-1, retinoblastoma, mSin3A, HDAC1, HAT1 together, BRG1, BAF155, HDAC2,...
Primary hepatocellular carcinoma (PHC), epidemiologically associated with chronic hepatitis B virus (HBV) infection, has historically been felt to be caused by the activation or introduction of an oncogene. However, transforming sequences from human PHC have not reproducibly isolated. In this paper, evidence is presented that suggests may result instead loss anti-oncogene. Seven 12 primary liver tumors tested against a panel restriction fragment length polymorphisms (RFLPs) demonstrated...
Journal Article A collection of tri- and tetranucleotide repeat markers used to generate high quality, resolution human genome-wide linkage maps Get access Val. C. Sheffield, Sheffield * 1Cooperative Human Linkage Center (CHLC) Departments Pediatrics *To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar James L. Weber, Weber 3Cooperative Marshfield Medical Research FoundationMarshfield, WI 54449 Kenneth H. Buetow, Buetow...
Abstract A key step in bringing gene expression data into clinical practice is the conduct of large studies to confirm preliminary models. The performance such confirmatory and transition requires that microarray from different laboratories are comparable reproducible. We designed a study assess comparability four will larger profiling confirmation project lung adenocarcinomas. To test feasibility combining across laboratories, frozen tumor tissues, cell line pellets, purified RNA samples...