A5044520462- HIV Research and Treatment
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- HIV/AIDS drug development and treatment
- HIV/AIDS Research and Interventions
- Immune Cell Function and Interaction
- Peptidase Inhibition and Analysis
- DNA and Nucleic Acid Chemistry
- MicroRNA in disease regulation
- RNA Research and Splicing
- Cytomegalovirus and herpesvirus research
- RNA Interference and Gene Delivery
- HIV, Drug Use, Sexual Risk
- Enzyme Production and Characterization
- CRISPR and Genetic Engineering
- Enzyme Catalysis and Immobilization
- Cancer-related gene regulation
- Extracellular vesicles in disease
- Protease and Inhibitor Mechanisms
- Protein Hydrolysis and Bioactive Peptides
- Signaling Pathways in Disease
- RNA modifications and cancer
- Pneumocystis jirovecii pneumonia detection and treatment
- PARP inhibition in cancer therapy
- Gold and Silver Nanoparticles Synthesis and Applications
Meharry Medical College
2016-2025
Walter de Gruyter (Germany)
2023
University of Nebraska Medical Center
2023
Google (Israel)
2023
Baidu (China)
2022-2023
Naver (South Korea)
2023
Nashville Oncology Associates
2023
Advisory Board Company (United States)
2023
Lesbian, Gay, Bisexual and Transgender Community Center
2022
Vanderbilt University
2011-2021
Pepsin−colloidal gold conjugates were prepared by a simple protein-friendly process and the enzymatic activity of bioconjugates is reported. The pepsin−gold are obtained mixing colloidal protein solutions at pH = 3 and, thereafter, centrifugation, washing, redispersion conjugate material in water. solution characterized UV−vis spectroscopy, fluorescence biocatalytic measurements while films bioconjugate solvent evaporation on suitable substrates further analyzed scanning electron microscopy...
The assembly of aqueous gold nanoparticles on the surface polyurethane (PU) spheres leading to [gold nanoparticle shell]−[polyurethane core] structures is demonstrated. polymer microspheres occurs through interaction nitrogens in with nanoparticles. Such direct obviates need perform additional modification microspheres, which an important step other polymer-based core−shell structure protocols. nanogold−PU material then conjugated enzyme pepsin, formation a new class biocatalyst. In relation...
We present herein details pertaining to the preparation of bioconjugates colloidal gold with aspartic protease from fungus Aspergillus saitoi (F-prot) and their characterization enzymatic activity. Simple mixing protein solutions under protein-friendly conditions (pH = 3) followed by centrifugation (to remove uncomplexed nanoparticles molecules) results in formation fungal protease−gold nanoparticle conjugates. The protein−gold bioconjugate was redispersed buffer solution indicated efficient...
Abstract Exosomes are important vehicles of intercellular communication that shape host responses to physiologic, tumorigenic, and pathogenic conditions. The composition function exosomes dynamic depends on the state condition cellular source. In prior work, we found semen (SE) from healthy donors who do not use illicit drugs potently inhibit HIV-1. Following donation, specimens either used immediately or frozen for at a later time. It has been shown short-term freezing no effect SE-mediated...
The main objective of this study was to examine effects cocaine on HIV-1 replication in primary CD4+ T cells. Cocaine a commonly used drug among positive individuals serves as cofactor for infection and progression acquired immunodeficiency syndrome (AIDS). Accumulating evidence suggest that increases cell cultures, peripheral blood mononuclear cells (PBMCs) animal models. Intriguingly, there are no studies cocaine-induced alterations serve the targets vivo. In report, we demonstrate...
The HIV-1 capsid protein (CA) facilitates reverse transcription and nuclear entry of the virus. However, CA's role in post-nuclear steps remains speculative. We describe a direct link between CA integration by employing inhibitor PF74 as probe coupled with biochemical analysis preintegration complexes (PICs) isolated from acutely infected cells. At low micromolar concentration, potently inhibited infection without affecting transcription. Surprisingly, markedly reduced proviral owing to...
MiR-124 is a highly expressed miRNA in the brain and regulates genes involved neuronal function. We report that miR-124 post-transcriptionally PARP-1. have identified conserved binding site of 3'-untranslated region (3'UTR) Parp-1 mRNA. demonstrate directly binds to 3'UTR mutations seed sequences abrogate between two RNA molecules. Luciferase reporter assay revealed activity dopaminergic cell model. Interestingly, overlapped with target sequence miR-125b, another post-transcriptional...
In the 21st century, we have witnessed three coronavirus outbreaks: SARS in 2003, MERS 2012, and ongoing pandemic disease 2019 (COVID-19). The search for efficient vaccines development repurposing of therapeutic drugs are major approaches COVID-19 research area. There concerns about evolution mutant strains (e.g., VUI – 202012/01, a United Kingdom), which can potentially reduce impact current vaccine drug trials. One promising approach to counter is “development effective broad-spectrum...
ABSTRACT Cleavage and polyadenylation specificity factor 6 (CPSF6) is part of the cellular cleavage I mammalian (CFIm) complex that regulates mRNA processing polyadenylation. CPSF6 also functions as a HIV-1 capsid (CA) binding host promotes viral DNA integration targeting into gene dense regions genome. However, effects on activity preintegration (PIC) - machinery carries out to establish infection unknown. To study CPSF6’s role in PIC function, we extracted PICs from cells depleted or...
ABSTRACT Cleavage and polyadenylation specificity factor 6 (CPSF6) is part of the cellular cleavage I mammalian (CFIm) complex that regulates mRNA processing polyadenylation. CPSF6 also functions as an HIV-1 capsid (CA) binding host to promote viral DNA integration targeting into gene-dense regions genome. However, effects on activity preintegration (PIC)—the sub-viral machinery carries out integration—are unknown. To study CPSF6’s role in PIC function, we extracted PICs from cells are...
Recent structural analyses indicate that localized regions of abnormal base pairing exist within RNA/DNA hybrids containing the HIV‐1 polypurine tract (PPT) and these distortions may play a role in PPT function. To examine this directly, we have introduced pyrrolo‐deoxycytosine (pdC), fluorescent, environmentally sensitive analog deoxycytosine (dC), into DNA strand PPT‐containing hybrids. Steady‐state fluorescence analysis reveals 11 nt from PPT–U3 junction is unpaired even absence reverse...
Substance abuse is a major barrier in eradication of the HIV epidemic because it serves as powerful cofactor for viral transmission, disease progression, and AIDS-related mortality. Cocaine, one commonly abused drugs among HIV-1 patients, has been suggested to accelerate progression. However, underlying mechanism remains largely unknown. Therefore, we tested whether cocaine augments HIV-1-associated CD4(+) T-cell decline, predictor We examined apoptosis resting T cells from HIV-1-negative...
The
Blood and semen are important body-fluids that carry exosomes for bioinformation transmission. Therefore, characterization of their proteomes is necessary understanding body-fluid-specific physiologic pathophysiologic functions. Using systematic multifactorial proteomic profiling, we characterized the exosome-free fractions from autologous blood three HIV-uninfected HIV-infected participants (total 24 samples). We identified exosome-based protein signatures specific to along with HIV-induced...
Suppressive mechanisms operating within T cells are linked to immune dysfunction in the tumor microenvironment. We have previously reported using adoptive cell immunotherapy models that tumor–bearing mice treated with a regimen of proteasome inhibitor, bortezomib - dipeptidyl boronate, show increased antitumor lymphocyte effector function and survival. Here, we identify mechanism for improved CD8 + following treatment. Intravenous administration at low dose (1 mg/kg body weight) wild-type or...