A5046504628- Muscle Physiology and Disorders
- Genetic and phenotypic traits in livestock
- Mycobacterium research and diagnosis
- Advanced Fluorescence Microscopy Techniques
- Cancer-related Molecular Pathways
- Ocular Oncology and Treatments
- Global Cancer Incidence and Screening
- Cardiomyopathy and Myosin Studies
- Photoacoustic and Ultrasonic Imaging
- Genomic variations and chromosomal abnormalities
- RNA Research and Splicing
- Optical Coherence Tomography Applications
- Microbial Metabolism and Applications
- Genetic Mapping and Diversity in Plants and Animals
- Neurogenetic and Muscular Disorders Research
- Muscle metabolism and nutrition
- Ubiquitin and proteasome pathways
- Cancer-related molecular mechanisms research
- Mitochondrial Function and Pathology
- Cancer Genomics and Diagnostics
- Genomics and Chromatin Dynamics
- Cervical Cancer and HPV Research
- Adipose Tissue and Metabolism
- Molecular Biology Techniques and Applications
- Congenital heart defects research
Université de Limoges
2016-2025
Inserm
1992-2025
Institut de Recherche pour le Développement
2022-2025
Institut d’Epidémiologie et de Neurologie Tropicale
2023-2024
Institut d’Epidémiologie Neurologique et de Neurologie Tropicale
2023-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2024
Université d'Abomey-Calavi
2023
University of Edinburgh
2023
Centre National Hospitalier et Universitaire Hubert Koutoukou MAGA
2023
Centre National de la Recherche Scientifique
1988-2022
We describe a new mouse frameshift mutation (Pax2(1Neu)) with 1-bp insertion in the Pax2 gene. This is identical to previously described human family renal-coloboma syndrome [Sanyanusin, P., McNoe, L. A., Sullivan, M. J., Weaver, R. G. & Eccles, (1995) Hum. Mol. Genet. 4, 2183-2184]. Heterozygous mutant mice exhibit defects kidney, optic nerve, and retinal layer of eye, homozygous embryos, development metanephric ventral regions inner ear severely affected. In addition, we observe deletion...
Proximal–distal outgrowth of the vertebrate limb bud is regulated by apical ectodermal ridge (AER), which forms at an invariant position along dorsal–ventral (D/V) axis embryo. We have studied genetic and cellular events that regulate AER formation in mouse. In contrast to implications from previous studies chick, we identified two distinct lineage boundaries mouse ectoderm prior using a Cre/loxP-based fate-mapping approach novel retroviral cell-labeling technique. One border transient limit...
Primary hepatocellular carcinoma (PHC), epidemiologically associated with chronic hepatitis B virus (HBV) infection, has historically been felt to be caused by the activation or introduction of an oncogene. However, transforming sequences from human PHC have not reproducibly isolated. In this paper, evidence is presented that suggests may result instead loss anti-oncogene. Seven 12 primary liver tumors tested against a panel restriction fragment length polymorphisms (RFLPs) demonstrated...
We have investigated the distribution of DNA methylatlon In chromosomes and nuclei normal individuals ICF (Immunodeficiency, Centromeric Instability Facial abnormalities) syndrome patients, using 5-methyl-cytosine monoclonal antibody. this syndrome, digestion with methyl-sensitive enzymes has previously shown a specific hypomethylation classical satellites located constitutive heterochromatin. The chromosome pattern confirms showing addition clear undermethylatlon facultative heterochromatin...
Germline mutations in the RB1 gene confer hereditary predisposition to retinoblastoma. We have performed a mutation survey of 232 patients with or non systematically explored all 27 exons and flanking sequences as well promotor. All types point are represented found unequally distributed along sequence. In population we studied, 3, 8, 18 19 preferentially altered. The range frequency detection germline is about 20%, indicating that other mechanisms inactivation should be involved. spectrum...
Using N-ethyl-N-nitrosourea-induced mutagenesis, we established a mouse model with novel form of neutropenia resulting from point mutation in the transcriptional repressor Growth Factor Independence 1 (Gfi1). These mice, called Genista, had normal viability and no weight loss, contrast to mice expressing null alleles Gfi1 gene. Furthermore, Genista very limited impact on lymphopoiesis or T- B-cell function. Within bone marrow (BM), resulted slight increase monopoiesis block terminal...
Northwest-African sheep represent an ideal case-study for assessing the potential impact of genetic homogenization as a threat to future traditional breeds that are adapted local conditions. We studied ten Algerian and Moroccan sheep, including three transboundary breeds, distributed over large part Maghreb region, which represents geographically historically coherent unit. Our analysis dataset involved carrying out Genome-wide SNP genotyping, revealed high level (ADMIXTURE, NetView,...
Abstract Background In Benin, a country in West Africa, breast cancer is the leading women, both terms of incidence and mortality. However, evidence on mortality its associated factors lacking this country. Our aim was to describe analyze clinical, histopathological, prognostic aspects Benin. Methods A descriptive analytical study carried out at CNHU-HKM CHU-MEL, two major tertiary referral hospitals for management located Cotonou, capital city All medical records with histological...
Copy number variations (CNV) are known to play a major role in genetic variability and disease pathogenesis several species including cattle. In this study, we report the identification characterization of CNV eight French beef dairy breeds using whole-genome sequence data from 200 animals. Bioinformatics analyses search for were carried out four different but complementary tools validated subset by both silico experimental approaches.We localization 4178 putative deletion-only,...
Multicolor multiphoton microscopy is experimentally demonstrated for the first time on a spectral bandwidth of excitation 300 nm (full width half maximum) thanks to implementation nanosecond supercontinuum (SC) source compact and simple with low repetition rate. The interest such wide bandwidth, never until now, highlighted in vivo: images glioma tumor cells stably expressing eGFP grafted brain mouse its blood vessels network labelled Texas Red(®) are obtained. These two fluorophores have...
Myostatin, a member of the TGFβ superfamily, is well known as potent and specific negative regulator muscle growth. Targeting myostatin signalling pathway may offer promising therapeutic strategies for treatment muscle-wasting disorders. In last decade, various myostatin-binding proteins have been identified to be able inhibit activity. One these GASP1 (Growth Differentiation Factor-Associated Serum Protein-1), protein containing follistatin domain multiple domains associated with protease...
Even though abundance of Hsp27 is the highest in skeletal muscle, relationships between expression HspB1 (encoding Hsp27) and muscle characteristics are not fully understood. In this study, we have analysed effect inactivation on mouse development phenotype. We generated a strain devoid protein by homologous recombination gene. The HspB1-/- was viable fertile, showing neither apparent morphological nor anatomical alterations. detected gender dimorphism with marked effects males, lower body...
Knowledge of population structure is essential to improve the management and conservation farm animal genetic resources. Microsatellites, which have long been popular for this type analysis, are more neglected in favor whole-genome single nucleotide polymorphism (SNP) chips that now available main farmed species. In study, we compared patterns derived from microsatellites inferred by SNPs, considering three pairs datasets sheep cattle. Population differentiation analyses (Fixation index,...
Abstract We present a new instrumental and computational pipeline named FAMOUS: f ast lgorithm for three-dimensional (3D) m ultiphoton microscopy of bi o medical str u cture s . This rests on MPM strategy combined with an original 3D post-processing approach. In the work, FAMOUS approach is devoted to imaging myosin assembly ultrastructure whole striated skeletal muscle unsliced. Raw recordings second harmonic generation (SHG) from point-spread functions (PSF) are led simultaneously all...
In recent years, several bovine genome sequencing projects were carried out with the aim of developing genomic tools to improve dairy and beef production efficiency sustainability. this study, we describe first French cattle variation dataset obtained by 274 whole genomes representing major breeds. This contains over 28 million single nucleotide polymorphisms (SNPs) small insertions deletions. Comparisons between results SNP array genotypes revealed a very high genotype concordance rate,...
Abstract Background Linkage disequilibrium (LD) is a key parameter to study the history of populations and identify fine map quantitative trait loci (QTL) it has been studied for many years in animal populations. The advent new genotyping technologies allowed whole-genome LD studies most cattle However, date, long-range (LRLD) between distant variants on genome not investigated detail cattle. Here, we present first comprehensive LRLD French beef by analysing data 672 Charolais (CHA), 462...
North Africa represents a rich and early reservoir of goat genetic diversity, from which the main African breeds have been derived. In this study, diversity four indigenous Algerian (i.e., Arabia, Makatia, M'Zabite Kabyle, with n = 12 for each breed) has investigated first time by genome-wide SNP genotyping; moreover in broader context, structuration Moroccan goats was explored (via FST, MDS, STRUCTURE, FineSTRUCTURE, BAPS, sPCA DAPC analyses). At national level, study revealed high level...
GASP-2 is a secreted multi-domain glycoprotein known as specific inhibitor of myostatin and GDF-11. Here we investigate the role on myogenesis effect its glycosylation activity.GASP-2 overexpression or knockdown by shRNAs were carried out C2C12 myoblasts cells. In silico analysis protein was performed to identify sites. We produced mouse recombinant in prokaryotic system obtain fully deglycosylated allowing us study importance this post-translational modification activity.Both mature...