A5034050775- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Cholinesterase and Neurodegenerative Diseases
- Chromatin Remodeling and Cancer
- DNA Repair Mechanisms
- Computational Drug Discovery Methods
- Genetics and Neurodevelopmental Disorders
- Single-cell and spatial transcriptomics
- Nuclear Structure and Function
- Cancer-related gene regulation
- Histone Deacetylase Inhibitors Research
- RNA Interference and Gene Delivery
- Renal and related cancers
- RNA regulation and disease
- Forensic and Genetic Research
- RNA and protein synthesis mechanisms
- Language and cultural evolution
- Gene Regulatory Network Analysis
- Animal Vocal Communication and Behavior
- Neuroscience and Neuropharmacology Research
- Protein Degradation and Inhibitors
Hebrew University of Jerusalem
2016-2025
Edmond and Lily Safra Children's Hospital
2016-2025
Institute of Life Sciences
2011-2025
Jerusalem University College
2020
Bar-Ilan University
2013
Nathan Kline Institute for Psychiatric Research
2008
Madison Group (United States)
2008
Singer (United States)
2008
RELX Group (United Kingdom)
2008
Advisory Board Company (United States)
2008
Methylating the Family Tree DNA sequences show a high level of similarities between humans and ancient hominids but degree to which there are differences methylated regions in their genomes that may explain phenotypic is unclear. Gokhman et al. (p. 523 , published online 17 April) demonstrate naturally degraded cytosines converted thymines can be used reconstruct methylomes. The results suggest methylation bone tissues modern set genes important for limb development.
The global impact of DNA methylation on alternative splicing is largely unknown. Using a genome-wide approach in wild-type and methylation-deficient embryonic stem cells, we found that can either enhance or silence exon recognition affects the more than 20% exons. These exons are characterized by distinct genetic epigenetic signatures. Alternative regulation subset these be explained heterochromatin protein 1 (HP1), which silences enhances position-dependent manner. We constructed an...
Tumors comprise functionally diverse subpopulations of cells with distinct proliferative potential. Here, we show that dynamic epigenetic states defined by the linker histone H1.0 determine which within a tumor can sustain long-term cancer growth. Numerous types exhibit high inter- and intratumor heterogeneity H1.0, levels correlating differentiation status, patient survival, and, at single-cell level, stem cell markers. Silencing promotes maintenance self-renewing inducing derepression...
To explore neuronal mechanisms underlying long-term consequences of stress, we studied stress-induced changes in the neuritic translocation acetylcholinesterase (AChE) splice variants. Under normal conditions, found synaptic AChE-S mRNA and protein neurites. Corticosterone, anticholinesterases, forced swim, each facilitated a rapid (minutes), yet long-lasting (weeks), shift from to normally rare AChE-R mRNA, promoted into neurites, induced enzyme secretion. Weeks after electrophysiological...
The fusion of the gametes upon fertilization results in formation a totipotent cell. Embryonic chromatin is expected to be able support large degree plasticity. However, whether this plasticity relies on particular conformation embryonic unknown. Moreover, functionally linked cellular potency has not been addressed. Here, we adapted fluorescence recovery after photobleaching (FRAP) developing mouse embryo and show that mobility core histones H2A, H3.1, H3.2 unusually high two-cell stage...
Embryonic stem cells (ESCs) exhibit unique chromatin features, including a permissive transcriptional program and an open, decondensed state. Induced pluripotent (iPSCs), which are very similar to ESCs, hold great promise for therapy basic research. However, the mechanisms by reprogramming occurs organization that underlies process largely unknown. Here we characterize compare epigenetic landscapes of partially fully reprogrammed iPSCs mouse embryonic fibroblasts (MEFs) serves as standard...
Stress induces long-lasting changes in neuronal gene expression and cholinergic neurotransmission, but the underlying mechanism(s) are incompletely understood. Here, we report that chromatin structure histone modifications causally involved this transcriptional memory. Specifically, AChE encoding acetylcholine-hydrolyzing enzyme acetylcholinesterase is known to undergo alternative splicing after stress. In mice subjected stress, identified two 5′ exons were down-regulated stress hippocampus,...
Resource26 July 2020Open Access Transparent process A Parkinson's disease CircRNAs Resource reveals a link between circSLC8A1 and oxidative stress Mor Hanan Department of Biological Chemistry, The Institute Life Sciences, Hebrew University Jerusalem, Israel Edmond Lily Safra Center for Brain Search more papers by this author Alon Simchovitz Nadav Yayon Shani Vaknine Roni Cohen-Fultheim Mina Everard Goodman Faculty Bar-Ilan University, Ramat Gan, Miriam Karmon Nimrod Madrer Talia Rohrlich...
Much remains unknown concerning the mechanism by which splicing machinery pinpoints short exons within intronic sequences and how factors are directed to their pre-mRNA targets. One probable explanation lies in differences chromatin organization between introns. Proteomic, co-immunoprecipitation, sedimentation analyses described here indicate that SF3B1, an essential component of U2 snRNP complex, is strongly associated with nucleosomes. ChIP-seq RNA-seq reveal SF3B1 specifically binds...
Abstract Changes in potential regulatory elements are thought to be key drivers of phenotypic divergence. However, identifying changes that underlie human-specific traits has proven very challenging. Here, we use 63 reconstructed and experimentally measured DNA methylation maps ancient present-day humans, as well six chimpanzees, detect differentially methylated regions likely emerged modern humans after the split from Neanderthals Denisovans. We show genes associated with face vocal tract...
Nucleosomes form heterogeneous groups in vivo, named clutches. Clutches are smaller and less dense mouse embryonic stem cells (ESCs) compared to neural progenitor (NPCs). Using coarse-grained modeling of the pluripotency Pou5f1 gene, we show that genome-wide clutch differences between ESCs NPCs can be reproduced at a single gene locus. Larger formation is associated with changes compaction internucleosome contact probability fiber. single-molecule tracking (SMT), further core histone protein...