A5022384420- RNA Research and Splicing
- Nuclear Structure and Function
- Prostate Cancer Treatment and Research
- Liver Disease Diagnosis and Treatment
- Genomics and Chromatin Dynamics
- Pancreatic function and diabetes
- Liver physiology and pathology
- Animal Genetics and Reproduction
- Xenotransplantation and immune response
- Drug-Induced Hepatotoxicity and Protection
- COVID-19 Clinical Research Studies
- Hepatitis C virus research
- Prostate Cancer Diagnosis and Treatment
- Circadian rhythm and melatonin
- Microfluidic and Capillary Electrophoresis Applications
- Metabolomics and Mass Spectrometry Studies
- RNA regulation and disease
- Metabolism, Diabetes, and Cancer
- Molecular Biology Techniques and Applications
- Genetics, Aging, and Longevity in Model Organisms
- Diet, Metabolism, and Disease
- DNA Repair Mechanisms
- 3D Printing in Biomedical Research
- Immune cells in cancer
- Advanced Proteomics Techniques and Applications
Hebrew University of Jerusalem
2012-2024
Institute of Life Sciences
2023
Technion – Israel Institute of Technology
2023
Soroka Medical Center
2022
University of Science and Technology of Benin
2018
Israel Aerospace Industries (Israel)
2018
Institute of Neurobiology
2017
Cornell University
2015
MRC Laboratory of Molecular Biology
2005-2009
Johns Hopkins University
2004
Summary The red colour of tomato ( Lycopersicon esculentum ) fruits is provided by the carotenoid pigment lycopene whose concentration increases dramatically during ripening process. A single dominant gene, Del , in mutant Delta changes fruit to orange as a result accumulation δ‐carotene at expense lycopene. cDNA for ε‐cyclase CrtL‐e ), which converts δ‐carotene, was cloned from tomato. primary structure CRTL‐E 71% identical homologous polypeptide Arabidopsis and 36% β‐cyclase, CRTL‐B. gene...
Microfluidic organ-on-a-chip technology aims to replace animal toxicity testing, but thus far has demonstrated few advantages over traditional methods. Mitochondrial dysfunction plays a critical role in the development of chemical and pharmaceutical toxicity, as well pluripotency disease processes. However, current methods evaluate mitochondrial activity still rely on end-point assays, resulting limited kinetic prognostic information. Here, we present liver-on-chip device capable maintaining...
The abundant flavonoid aglycone, naringenin, which is responsible for the bitter taste in grapefruits, has been shown to possess hypolipidemic and anti-inflammatory effects both vitro vivo. Recently, our group demonstrated that naringenin inhibits hepatitis C virus (HCV) production, while others its potential treatment of hyperlipidemia diabetes. However, suffers from low oral bioavailability critically limiting clinical potential. In this study, we demonstrate solubility enhanced by...
The kidney plays a critical role in fluid homeostasis, glucose control, and drug excretion. Loss of function due to drug-induced nephrotoxicity affects over 20% the adult population. proximal tubule is complex vascularized structure that particularly vulnerable nephrotoxicity. Here, we introduce model human spheroids with integrated tissue-embedded microsensors for oxygen, glucose, lactate, glutamine, providing real-time assessment cellular metabolism. Our shows both immunosuppressive...
Mutations in the Caenorhabditis elegans unc-84 gene cause defects nuclear migration and anchoring. We show that endogenous UNC-84 protein colocalizes with Ce-lamin at envelope localization of requires Ce-lamin. also during mitosis, remains periphery until late anaphase, similar to known inner membrane proteins. is first detected 26-cell stage thereafter present most cells development adults. properly expressed unc-83 andanc-1 lines, which have phenotypes tounc-84, suggesting neither...
The liver is the main organ responsible for modification, clearance, and transformational toxicity of most xenobiotics owing to its abundance in cytochrome P450 (CYP450) enzymes. However, scarcity variability primary hepatocytes currently limits their utility. Human pluripotent stem cells (hPSCs) represent an excellent source differentiated hepatocytes; however, current protocols still produce fetal‐like with limited mature function. Interestingly, fetal acquire CYP450 expression only...
Export Altered glucose reabsorption via the facilitative transporter 2 (GLUT2) during diabetes may lead to renal proximal tubule cell (RPTC) injury, inflammation, and interstitial fibrosis. These pathologies are also triggered by activating cannabinoid-1 receptor (CB1R), which contributes development of diabetic nephropathy (DN). However, link between CB1R GLUT2 remains be determined. Here, we show that chronic peripheral blockade or genetically inactivating CB1Rs in RPTCs ameliorated...
GLUT2 is a facilitative glucose transporter, expressed in polarized epithelial cells of the liver, intestine, kidney and pancreas, where it plays critical role homeostasis. Together with SGLT1/2, mediates absorption metabolic tissues, can be translocated apically upon high exposure. To track subcellular localization dynamics GLUT2, we created an mCherry-hGLUT2 fusion protein multicellular cysts, major site reabsorption. Live imaging enabled us to avoid artefactual fixed confirm apical model....
Caenorhabditis elegans mtf-1 encodes matefin, which has a predicted SUN domain, coiled-coil region, an anti-erbB-2 IgG and two hydrophobic regions. We show that matefin is nuclear membrane protein colocalizes in vivo with Ce-lamin, the single lamin C. , binds Ce-lamin vitro but does not require for its localization. Matefin detected all embryonic cells until midembryogenesis thereafter only germ-line cells. Embryonic maternally deposited, first known to have germ line-restricted expression....
Emerin belongs to the LEM-domain family of nuclear membrane proteins, which are conserved in metazoans from C. elegans humans. Loss emerin humans causes X-linked form Emery-Dreifuss muscular dystrophy(EDMD), but disease mechanism is not understood. We have begun address function elegans, a genetically tractable nematode. The gene (emr-1) elegans. detect Ce-emerin protein envelopes all cell types except sperm, and find that co-immunoprecipitates with Ce-lamin embryo lysates. show for first...
Abstract
Sensor-integrated liver chip unravels risks of drug-induced fatty disease associated with prescription drug use.
Gp210 is an evolutionarily conserved membrane protein of the nuclear pore complex (NPC). We studied phenotypes produced by RNAi-induced downregulation gp210 in both human (HeLa) cells and Caenorhabditis elegans embryos. HeLa cell viability requires activity. The dying accumulated clustered NPCs aberrant structures at envelope, suggesting that required directly or indirectly for formation dilation as well anchoring structural integrity mature NPCs. Essential roles were confirmed C. elegans,...
Background: Viral infection is associated with a significant rewire of the host metabolic pathways, presenting attractive targets for intervention. Methods: We chart response lung epithelial cells to SARS-CoV-2 in primary cultures and COVID-19 patient samples perform vitro metabolism-focused drug screen on infected different strains virus. observational analysis Israeli patients hospitalized due comparative epidemiological from cohorts Italy Veteran’s Health Administration United States. In...