A5012485520- Plant tissue culture and regeneration
- Transgenic Plants and Applications
- Plant Genetic and Mutation Studies
- Plant Molecular Biology Research
- Berberine and alkaloids research
- Plant Gene Expression Analysis
- Plant-Microbe Interactions and Immunity
- Seed Germination and Physiology
- Plant Parasitism and Resistance
- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- GABA and Rice Research
- Lipid metabolism and biosynthesis
- Algal biology and biofuel production
- Photosynthetic Processes and Mechanisms
- Ginseng Biological Effects and Applications
- RNA and protein synthesis mechanisms
- Experimental Learning in Engineering
- Liver Disease Diagnosis and Treatment
- Light effects on plants
- Engineering Education and Curriculum Development
- Molecular Biology Techniques and Applications
- Drug-Induced Hepatotoxicity and Protection
- Hepatitis C virus research
- Natural Antidiabetic Agents Studies
Northeastern University
2012-2025
Boston University
2024-2025
Universidad del Noreste
2005-2024
Pennsylvania State University
2010
Cornell University
2002
The abundant flavonoid aglycone, naringenin, which is responsible for the bitter taste in grapefruits, has been shown to possess hypolipidemic and anti-inflammatory effects both vitro vivo. Recently, our group demonstrated that naringenin inhibits hepatitis C virus (HCV) production, while others its potential treatment of hyperlipidemia diabetes. However, suffers from low oral bioavailability critically limiting clinical potential. In this study, we demonstrate solubility enhanced by...
The recent discovery of the mode action CRISPR/Cas9 system has provided biologists with a useful tool for generating site-specific mutations in genes interest. In plants, site-targeted are usually obtained by stable transformation Cas9 expression construct into plant genome. efficiency introducing interest can vary considerably depending on specific features constructs, including source and nature promoters terminators used gene guide RNA, sequence nuclease itself. To optimize target...
The Catharanthus roseus plant is the source of many valuable terpenoid indole alkaloids (TIAs), including anticancer compounds vinblastine and vincristine. Transcription factors (TFs) are promising metabolic engineering targets due to their ability regulate multiple biosynthetic pathway genes. To increase TIA biosynthesis, we elicited transcriptional activators (ORCAs other unidentified TFs) with hormone, methyl jasmonate (MJ), while simultaneously silencing expression repressor ZCT1....
Abstract Key message A GLK homologue was identified and functionally characterized in Catharanthus roseus . Silencing CrGLK with VIGS or the chloroplast retrograde signaling inducer lincomycin increased terpenoid indole alkaloid biosynthesis. is sole source of chemotherapeutic alkaloids (TIAs) vinblastine vincristine. TIA pathway genes, particularly genes vindoline pathway, are expressed at higher levels immature versus mature leaves, but molecular mechanisms responsible for this...
Abstract The production of pharmaceutically important terpenoid indole alkaloids (TIAs) from Catharanthus roseus is partly regulated at the transcriptional level. In this study, limitations in TIA biosynthesis C. hairy root cultures were assessed through gene expression profiling and precursor feeding. transcript levels key pathway genes (G10h, Tdc, Str, Sgd) metabolite associated with (tryptamine, loganin, secologanin, strictosidine, ajmalicine, serpentine, tabersonine) monitored using...
The effects of methyl jasmonate (MJ) dosage on terpenoid indole alkaloid (TIA) biosynthesis in Catharanthus roseus are correlated with the relative levels specific MJ-responsive transcription factors. In this study, expression factors (Orca, Zct, Gbf, Myc2, At-hook, and Wrky1), TIA pathway genes (G10h, Tdc, Str, Sgd), metabolites (secologanin, strictosidine, tabersonine) were investigated C. hairy root cultures elicited a range MJ dosages (0-1,000 µM) during mid-exponential growth. highest...
The Catharanthus roseus plant is the exclusive source of valuable anticancer terpenoid indole alkaloids, vinblastine (VB) and vincristine (VC). recent availability transcriptome genome resources for C. necessitates a fast reliable method studying gene function. In this study, we developed an Agrobacterium-mediated transient expression to enable functional study genes rapidly in planta, conserving compartmentalization observed VB VC pathway. We focused on 1) improving transformation (syringe...
Plant cell and tissue cultures are a scalable controllable alternative to whole plants for obtaining natural products of medical relevance. Cultures can be optimized high yields desired metabolites using rapid profiling assays such as HPLC. We describe an approach establishing assay culture expression systems novel microscale LC-UV-MS-NMR platform, designed acquire both MS NMR each at their optimal sensitivity, by nanosplitter from 4 mm analytical HPLC columns, offline microdroplet NMR. The...
The California poppy, Eschscholzia californica, produces benzophenanthridine alkaloids (BPAs), an important class of biologically active compounds. Cell cultures E. californica were investigated as alternative and scalable method for producing these valuable compounds; treatment with yeast extract increased production from low levels to 23 mg/g dry weight (DW) BPAs. A shotgun proteomic analysis cell was undertaken explore changes in metabolism associated enhanced BPA production. We...
Abstract Cys 2 /His ‐type (C H ) zinc finger proteins, such as ZCT1, are an important class of transcription factors involved in growth, development, and stress responses plants. In the medicinal plant Catharanthus roseus, factor (ZCT) family represses monoterpenoid indole alkaloid (MIA) biosynthetic gene expression . Here, we report analysis ZCT1 promoter, which contains several hormone‐responsive elements. is responsive to not only jasmonate, was previously known, but also induced by...
Abstract The potential for the feedback inhibition of indole alkaloid synthesis was investigated by spiking suspension cultures Catharanthus roseus with 0, 9, or 18 mg/L ajmalicine on day 0. production ajmalicine, catharanthine, and serpentine were inhibited in a dose‐dependent manner. transient as exogenous ultimately either metabolized medium within cell. addition neutral resin has previously been shown to enhance production. To minimize product metabolism, Amberlite XAD‐7 added...
The abundant flavonoid aglycone, naringenin, which is responsible for the bitter taste in grapefruits, has been shown to possess hypolipidemic and anti-inflammatory effects both vitro vivo.Recently, our group demonstrated that naringenin inhibits hepatitis C virus (HCV) production, while others its potential treatment of hyperlipidemia diabetes.However, suffers from low oral bioavailability critically limiting clinical potential.In this study, we demonstrate solubility enhanced by...